Techniques of liver decellularization
Journal Title: Moldovan Medical Journal - Year 2018, Vol 61, Issue 4
Abstract
Background: The growth of the number of people who need the liver transplant and the insufficiency of organ donors, have urged the advancement in bioengineering through the development of new therapeutic strategies which involve generation of functional artificial organs, obtained by the decellularization technology and creation of extracellular matrix and their subsequent recellularization. Material and methods: Rat livers (n = 9) served as the object of this study which were subjected to decellularization with sodium dodecyl sulfate (SDS) solution 0.1 and 0.5% and the combination of SDS 0.1 to 0.5% and anticoagulant. Subsequently, the extraction of nucleic acids was performed according to the protocol QIAamp Blood Mini Kit (2003). The histological analysis was performed with haematoxylin-eosin (H-E) and the quantification of hydroxyproline via spectrophotometric method. Results: After the liver tissue decellularization we obtained the matrix of decellularized liver. The genetical, biochemical and histological analysis revealed a better decellularization by the combined method versus the method with SDS solution only. Conclusions: The quantification of nucleic acids content, hydroxyproline and the histological analysis of decellularized matrix with anticoagulant and detergent SDS method, suggested a more efficient decellularization of liver tissue segments and we achieved a decellularized bioconstruction for recellularization.
Authors and Affiliations
Mariana Jian, Vitalie Cobzac, Ivan Moghildea, Victor Popescu, Viorel Nacu
Informing national salt reduction strategy
Background: Excessive salt intake is associated with noncommunicable diseases, especially with hypertension, cardiovascular disease and stroke. Implementation of comprehensive and effective strategies can reduce salt int...
Methodology of computed tomography of maxillofacial and craniocerebral regions by means of vertical fixation of the examined objects
Background: The research aims at identifying new methodological approaches to the study of morphological patterns of prenatal development of the human upper jaw. Material and methods: A working model has been developed a...
B-cell lymphoma-2 receptor in human primary breast and its lymph node metastases: more than a surrogate marker
Background: Due to its anti-apoptotic and anti-proliferative contradictory functions, BCL2 role in breast carcinoma progression is not clearly understood. The purpose of this study was to highlight BCL2 expression during...
Fundamental aspects of cardiovascular regulation in predisposition to atrial fibrillation
Background: Atrial fibrillation is the most common sustained arrhythmia in cardiology. The structural factors leading to atrial fibrillation are well known, but there should be also regarded the functional factors. In 20...
From body mass index to body composition analysis in diagnostic of childhood obesity
Background: The prevalence of obesity increased worldwide in children and adolescents from 1975 to 2016. The recent increase in childhood obesity has led to an interest in the question of which definitions should be used...