Techniques of liver decellularization

Journal Title: Moldovan Medical Journal - Year 2018, Vol 61, Issue 4

Abstract

Background: The growth of the number of people who need the liver transplant and the insufficiency of organ donors, have urged the advancement in bioengineering through the development of new therapeutic strategies which involve generation of functional artificial organs, obtained by the decellularization technology and creation of extracellular matrix and their subsequent recellularization. Material and methods: Rat livers (n = 9) served as the object of this study which were subjected to decellularization with sodium dodecyl sulfate (SDS) solution 0.1 and 0.5% and the combination of SDS 0.1 to 0.5% and anticoagulant. Subsequently, the extraction of nucleic acids was performed according to the protocol QIAamp Blood Mini Kit (2003). The histological analysis was performed with haematoxylin-eosin (H-E) and the quantification of hydroxyproline via spectrophotometric method. Results: After the liver tissue decellularization we obtained the matrix of decellularized liver. The genetical, biochemical and histological analysis revealed a better decellularization by the combined method versus the method with SDS solution only. Conclusions: The quantification of nucleic acids content, hydroxyproline and the histological analysis of decellularized matrix with anticoagulant and detergent SDS method, suggested a more efficient decellularization of liver tissue segments and we achieved a decellularized bioconstruction for recellularization.

Authors and Affiliations

Mariana Jian, Vitalie Cobzac, Ivan Moghildea, Victor Popescu, Viorel Nacu

Keywords

Related Articles

Overview of evolution of premature mortality from major cardiovascular diseases in the Republic of Moldova, 2003-2015

Background: Cardiovascular disease is the leading cause of mortality at the global and national level. Ischeamic heart diseases, cerebrovascular diseases and hypertensive heart disease together identify a highest proport...

Disturbance of bioelectric transmission in carcinogenesis

Background: Despite significant financial resources invested in the field of cancer research, there has been a steady increase in the registration of new cases of malignant neoplasms. Modern technical capabilities for an...

Usage of cardiotonic drugs at the intensive care units

Background: Circulation insufficiency is one of the most common dysfunctions in the patients admitted to the intensive care units (ICU) [1]. These patients need an intravenous (IV) vasoactive drug administration to optim...

Monoinvasion with toxocara canis in children

Background: Toxocariasis is a clinical notion that reflects human involvement with the Toxocara larvae in the second stage of development. Its typical syndromes are Larvae Migrans Visceralis, ocular toxocarosis , neurolo...

Acupuncture, Moxibustion and Chinese herbs in prevention of nosocomial infection in patients with acute cerebrovascular accident

Background: Nosocomial infection is a current medical issue, particularly in patients with acute cerebrovascular accident. The present study purpose isto evaluate the effectiveness of acupuncture, Chinese herbs and moxib...

Download PDF file
  • EP ID EP479434
  • DOI 10.5281/zenodo.2222303
  • Views 321
  • Downloads 0

How To Cite

Mariana Jian, Vitalie Cobzac, Ivan Moghildea, Victor Popescu, Viorel Nacu (2018). Techniques of liver decellularization. Moldovan Medical Journal, 61(4), 21-24. https://europub.co.uk/articles/-A-479434