The Effect of Glucose 6-Phosphate Dehydrogenase Deficiency (G6PDd) and Haemoglobin Variants on Malaria in Port Harcourt, Rivers State, Nigeria
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 12
Abstract
The burden of the disease due to malaria is believed to share the same geographical distribution that correlates with G6PD deficiency and sickle cell haemoglobin (HbSS) due to protective advantage against malaria parasites. This study was aimed at studying the interactions between G6PD and haemoglobin variants in sub-clinical malaria infected pregnant women. Five millilitres (5ml) of venous blood was collected aseptically into 1% diamine tetraacetic acid bottle and carefully mixed for the analysis. G6PD activity was measured quantitatively with 3000 BSA spectrophotometer using the Randox G6PD kit. Malaria parasite density was determined microscopically using thick and thin Giemsa stained blood smears. Determination of haemoglobin variants was done using cellulose acetate membrane electrophoresis with Tris-EDTA-borate buffer (pH 8.9). All results were analysed using measure of significance taken as p values, that is (p>0.05) was regarded as insignificance while p<0.05 was regarded as significant. A total of eight hundred and twenty-eight (828) participated in the study. Of these, five hundred and seven 507 (61.2%) were infected with malaria parasite and three hundred and twenty-one (321) (38.8%) served as controls (uninfected subjects). HbAA genotype took dominance in malaria parasite infestation, 379 (74.8%), then HbAS 126 (24.9%) and HbSS 2 (0.4%). The mean age of the study participant was 29.5+5.31 years. Also, five hundred and seventeen 517 (62.4%) were G6PD deficient out of the eight hundred and twenty eight participants. The mean value of G6PD levels for HbSS was significantly elevated at 12.20+0.30u/gHb compared to HbAS, 6.28+0.16u/gHb and HbAA, 6.60+0.07u/gHb. It is therefore concluded that coinheritance of G6PD and HbS has no advantage over single inheritance of HbS variant.
Authors and Affiliations
Susanna O. Akwuebu
Keywords
Related Articles
- EP ID EP490119
- DOI -
- Views 51
- Downloads 0
Assessment of Cardiovascular Autonomic Functions in Normal Healthy Young Adults
The portion of the nervous system that controls most visceral functions of the body is called the Autonomic Nervous System (ANS). The effects of the ANS on heart rate are called “chronotropic effects”. Briefly, sympathet...
A Comparative Study of Laparoscopic Appendicectomy and Open Appendicectomy
Aims and Objectives: Comparative study between laparoscopic appendicectomy and open appendicectomy in terms of safety and benefits with respect to duration of operation, post-operative pain, resumption of oral diet, dura...
A Comparative Study on Maternal and Fetal Outcome in Cases of Placenta Previa with Previous Cesarean Section and Without Previous Cesarean Section
Placenta previa is the major cause of antepartum haemorrhage that causes serious morbidity and mortality to both fetus and mother. Objective[s]: To compare the antepartum, intrapartum, postpartum complications in placent...
Evaluation of Awareness of Common Eye Diseases among General Population of Bihar
Objectives: The main objective of this study is to assess the awareness level of common eye related problems in population of Bihar. Material & Methods: a cross-sectional type of study was conducted on 422 general popula...
Treatment Outcomes in Concomitant Esotropias
Purpose: To assess the outcome of various treatment modalities of concomitant esotropias. Design: Prospective randomized population based study. Materials & Methodology: 100 patients who attended squint clinic of Regiona...