The influence of cotinine on the non-small-cell lung cancer line A549
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2009, Vol 63, Issue
Abstract
The aim of this investigation was to determine the influence of cotinine on the non-small-cell lung cancer line A549. The material for the study was the A549 cell line. The cells were subjected to 24-h incubation with cotinine at doses of 18 and 36 ng/ml. Control cells were incubated under analogous conditions without the addition of cotinine. Cell viability was determined and the cells were Mayer hematoxylin stained, embedded in epon, and observed under an electron microscope. A fluorescent method was used to evaluate F-actin. Cotinine’s action resulted in alteration of the cytoskeleton, with changes in the organization of F-actin. Also observed was A549 cell death, presumably due to the activation of apoptotic and mitotic catastrophe pathways. Moreover, the results at the ultrastructural level suggest that cotinine influences not only the nucleus and cell shape by acting on the actin cytoskeleton, but also plays a crucial role in changes which include the remaining cellular organelles. Until now, research has been focused on determining the action of constituents of cigarette smoke, mainly nicotine. From this point of view, studies on cotinine seem justified. Results of the study allow us to suppose that cotinine, through F-actin, can influence the size and shape of non-small-cell lung cancer cells. There are some suggestions that cells of the A549 cell line can undergo death through apoptotic and mitotic catastrophe pathways as a result of cotinine’s action.
Authors and Affiliations
Jakub Nowak, Alina Grzanka, Maciej Gagat, Agnieszka Żuryń
Keywords
Related Articles
- EP ID EP66286
- DOI -
- Views 220
- Downloads 0
Stężenie karnityny w surowicy jest obniżone u pacjentów chorych na boreliozę z Lyme
Limfocyty Th17 w zakażeniach bakteryjnych
Komórki Th17 są niedawno odkrytą subpopulacją pomocniczych limfocytów T CD4+. Wykazano, że komórki te przyczyniają się do uszkodzeń w przebiegu licznych chorób tła immunologicznego oraz odgrywają istotną rolę w odpornośc...
Conjugated linolenic acids (CLnA, super CLA) – natural sources and biological activity
Polyunsaturated fatty acids (PUFA) have a wide range of biological activity. Among them conjugated fatty acids are of great interest. Conjugated linoleic acids (CLA), which exert a multidirectional health-benefiting infl...
Genetic methods in diagnosis of hematooncological disorders
The development of various analitic techniques like classical cytogenetics, molecular cytogenetics and molecular methods has greatly improved our ability to understand the basis of cancer development, that can be helpful...
Nitroksydy jako antyoksydanty – możliwości ich zastosowania w celach chemioprewencyjnych oraz radioprotekcyjnych
Nitroksydy jako trwałe rodniki organiczne początkowo wykorzystywane były jako znaczniki spinowe w spektroskopii elektronowego rezonansu paramagnetycznego (electron paramagnetic resonance – EPR) w badaniach takich param...