TITLE: Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji

Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2016, Vol 8, Issue 2

Abstract

A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars.The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1). The highest bud rooting of buds occurred on MS medium added with 1 mg/l NAA. The percentage of rooting varied from 16.66 to 75%. The highest percentage of survival plants after acclimatization were 41.73 and 57.63 for Beji and Maazoun respectively.

Authors and Affiliations

Rhimi Awatef, Boussaid Mohamed

Keywords

Related Articles

Assessment of the Biomedical Wastewater Treatment Station of the National University Hospital of Cotonou Benin (West Africa)

Poor management of biomedical wastewater is a major threat for the environment and the public health. This study aims to assess the activated sludge wastewater station of CNHU-HKM of Cotonou, the biggest hospital in th...

A Modified Genomic DNA Extraction Method from Leaves of Sunflower for PCR Based Analyzes

Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysacch...

Investigation on potential of Suaeda fruticosa as a source of edible oil

Given the extent of saline lands in Iran, cultivation and utilization of halophytes and salt tolerant species under the condition that both water and soil are saline could be a viable option in production and extraction...

Biology of the deepwater rose shrimp Parapenaeus longirostris (Lucas, 1846) from the Oranian coast of Western Mediterranean)

The Crustaceans have an important place thanks to their value bargains (market).Owing to the strong demand on the world market of the Crustacean D├ęcapodes, the fishing of this resource doesn't stop developing, with a p...

The study and analysis of models and methods of academic achievement assessment in a Corporate University

Assessment plays an integral role in education process, since without conducting a correct and reliable assessment, it is safe to say that the education process is left unfinished and does not come to an end .One of the...

Download PDF file
  • EP ID EP38739
  • DOI -
  • Views 58
  • Downloads 0

How To Cite

Rhimi Awatef, Boussaid Mohamed (2016). TITLE: Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji. Journal of Biodiversity and Environmental Sciences (JBES), 8(2), -. https://europub.co.uk/articles/-A-38739