Biotecnología Vegetal

Biotecnología Vegetal

Basic info

  • Publisher: Universidad Central Marta Abreu de Las Villas
  • Country of publisher: cuba
  • Date added to EuroPub: 2017/Sep/28

Subject and more

  • LCC Subject Category: Agricultural Science, Plant Sciences
  • Publisher's keywords: Embryogenesis, Organogenesis, Plant Breeding, Mass Propagation, Plant Biotechnology, Plant in Vitro Culture
  • Language of fulltext: english, spanish, catalan
  • Time from submission to publication: 12 weeks

Publication charges

  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? No

Open access & licensing

  • Type of License: CC BY-NC
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2001
  • Does the author retain unrestricted copyright? False
  • Does the author retain publishing rights? False

Best practice polices

  • Permanent article identifier: None
  • Content digitally archived in: LOCKSS,
  • Deposit policy registered in: None

This journal has '417' articles

In silico identification of DELLA proteins in plants of Coffea arabica L. and Ananas comosus L. Merr

In silico identification of DELLA proteins in plants of Coffea arabica L. and Ananas comosus L. Merr

Authors: Maita Ávila Espinosa , Andre Almeida Lima , Solange Aparecida Ságio , Carla Priscila Coelho
Year: 2012, Volume: 12, Number: 3
(1 downloads)
Abstract

Gibberellins control various aspects of plants growing and development, including seed germination, stem elongation, leaves expansion, flower and seed development. They are also involved in phase changes in some species, as from vegetative transition to reproductive. Most of these aspects seem to be regulated by DELLA proteins, which are negative regulators of the induced growth by GAs. The asynchronism in flowering processes of coffee and pineapple plants is recognized. This is also directly associated with the low quality of productions of both species. Studies that allow understanding this process are very important. This article shows a characterization in silico of supposed DELLA proteins in the database of CAFEST genome, gene bank of pineapple and GenBank to understand the role of DELLA proteins in the coffee and pineapple flowering processes. Structural similarity and expression profile in silico showed by probable DELLA genes in coffee corroborated the classification of DELLA proteins and suggest the presence of two of them in this specie. In pineapple, after an exhausted search, none of the bank genes proteins from this specie and the GenBank showed DELLA motifs in the four domains with structural functions related to the control of GAs. Nevertheless, in the future these analyses should be repeated due to the possibility that the corresponding sequences of these genes may not be present in the analyzed database.

Keywords: bioinformatics, CAFEST, flowering process, gibberellins
Effect of substrate type on the acclimatization of in vitro plants of Bambusa vulgaris

Effect of substrate type on the acclimatization of in vitro plants of Bambusa vulgaris

Authors: Ortelio Hurtado, Marisol Freire-Seijo, Michel Leiva-Mora, Yudith García-Ramírez
Year: 2012, Volume: 12, Number: 2
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Abstract

Bambusa vulgaris var. vulgaris has several uses. The biotechnological propagation can be an alternative to reach the demand of planting material. This investigation was aimed to determine the influence of substrate type on the acclimatization of in vitro plants. Two main components (worm humus (HL) and zeolite (Z)) were used to produce the substrate. These were combined (HL 70% and 30% Z, HL 60% and 40% Z, HL 50% and Z 50%, HL 80% and 20% Z). Worm compost and zeolite substrate was used as control. In vitro plants from rooting phase were acclimatized for 90 days. The number of roots per plant was assessed every 10 days (root length, number of shoots per plant, plant height and leave number). Survival was determined after 30 days of planting. Plant fresh and dry mass (g) were determined, subsequently, after 90 days of culture. Results demonstrated that the type of substrate influenced on the acclimatization of plants of B. vulgaris in greenhouse. The highest survival (%) ,number and length of roots as well as number of shoots per plant, fresh and dry mass of roots and cauline area, were achieved when the zeolite and worm humus were combined and by increasing the proportion of this last product.

Keywords: bamboo, worm humus, zeolite
Foliar anatomy and morphological characterization of Bambusa vulgaris in the acclimatization phase

Foliar anatomy and morphological characterization of Bambusa vulgaris in the acclimatization phase

Authors: Ortelio Hurtado, Marisol Freire-Seijo, Michel Leiva-Mora, Yudith García-Ramírez
Year: 2012, Volume: 12, Number: 3
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Abstract

This study was aimed to achieve the morphological and anatomical characterization of in vitro plants of Bambusa vulgaris Schrad. ex Wendl during acclimatization. The morphological development of the leaf and root in plants acclimatized with 0 and 90 days after planting, as well as field plants with more than 15 years of planting were considered. Results showed important differences between the structures of the leaf in the evaluated periods. Both presented adaxial and abaxial monostratified epidermis, with presence of trichomes, stomata, micro-hairs in the intercostal area. The abaxial surface showed silica and papilla cells. In the analysis of the cross section of leaf blade B. vulgaris were observed bulliform and fusoid cells, confirming the presence of the xylem, phloem, epidermis, cuticle, vascular bundles, the chlorenchyma showing differences among the development stages. The longitudinal cut to the root-stem junction demonstrated a further development in plants acclimatized for 90 days.

Keywords: bamboo, trichomes, leaf blade, stomata, epidermis
Selection of Azotobacter chroococcum strains for its application on in vitro pineapple cv. ‘Cayena lisa’ plants acclimatization

Selection of Azotobacter chroococcum strains for its application on in vitro pineapple cv. ‘Cayena lisa’ plants acclimatization

Authors: Rayza M. González Rodríguez , Alitza Iglesias , José Calos Lorenzo , Bernardo Dibut
Year: 2012, Volume: 12, Number: 3
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Abstract

Azotobacter chroococcum is a rhizosphere bacterium that has been used for decades as a plant growth stimulator in a wide range of crops. It is able to fix nitrogen and produce growth promoting substances. In the present work we show the results to determine the effectiveness of the application of different strains of A. chroococccum on acclimatization of in vitro pineapple plants. Six strains were evaluated from the strain collection of INIFAT. For its multiplication and application the Cuban standard for the production of A. chroococcum was used. We evaluated the following indicators: survival, plant height, leaf number, root length, fresh weight and dry weight. Strain INIFAT 5 proved to be the greatest stimulation occurred in most of the variables analyzed and it was selected for further study as the most effective strain for acclimatization of in vitro pineapple plants.

Keywords: Ananas comosus L., biofertilizer, plant growth promotion bacteria
Effect of 2,4-D concentration in somatic embryos formation of cuban soybeans varieties

Effect of 2,4-D concentration in somatic embryos formation of cuban soybeans varieties

Authors: Idalmis Bermúdez-Caraballoso , Teresa Del Socorro Blanco , Jorge Pérez-Pérez , Lourdes R. García , Novisel Veitía , Raúl Collado , Damaris Torre...
Year: 2012, Volume: 12, Number: 2
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Abstract

Developing new breeding programs of soybean crops needs an efficient systems for plants regeneration. Eventhough due this is impossible taking into account its genotype dependent condition. This study aimed to: determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the formation of somatic embryos in soybean Cuban varieties (Incasoy-1, Incasoy-24, Incasoy-27 and Incasoy-35). Immature cotyledons were used as plant material. Three treatments were established according to the concentrations of 2,4-D (30, 40 and 50 mg l -1 ). The number of somatic embryos per immature cotyledon were evaluated after 30 days. The necessary concentration of 2,4-D to obtain the mayor formation of embryos depended on the genotypes (30 mg l -1 for Incasoy-24; 40 mg l -1 for Incasoy-27 and Incasoy-35; 40 and 50 mg l -1 for Incasoy-1). Depending on the results obtained the concentration 40 mg l -1 for the cuban varieties was selected The Incasoy-24 was the exception since it responded better with a concentration of 30 mg l -1 . These results favor the development of new programs for genetic breedig of soybeans in Cuba.

Keywords: Glycine max, plants regeneration, somatic embryogenesis
Detection and quantification of quitinasa in banana (Musa spp.) leaves inoculated with Mycosphaerella fijiensis

Detection and quantification of quitinasa in banana (Musa spp.) leaves inoculated with Mycosphaerella fijiensis

Authors: Cynthia Sánchez-García,* Mileidy Cruz-Martín, Yelenys Alvarado-Capó, Luis Rojas, Michel Leiva- Mora, Mayra Acosta-Suárez, Berkis Roque
Year: 2012, Volume: 12, Number: 2
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Abstract

The knowledge of the role of chitinase enzyme in Musa spp- Mycosphaerella fijiensis interaction is still very limited. Therefore, their study is of great interest, mainly in breeding programs. The aim of this study was to detect and quantify chitinase enzyme in leaves of ‘Grande naine’ and ‘Calcutta 4’ inoculated with M. fijiensis in greenhouse conditions. This enzyme accumulation was detected in leaves of both genotypes and at different times after inoculation using agarose gel with glycol chitin as a substrate and it was revealed by using Calcoflour brightener. It was noted that in ‘Calcutta 4’ plants there was an increase in chitinase activity in inoculated compared to non-inoculated plants at all times studied. In ‘Grande naine’ inoculated plants this difference was only observed at 2 h and at 3 days after inoculation respect to non-inoculated plants. At each time, the highest values of enzyme concentration corresponded to samples of the resistant genotype. It was shown that the accumulation of this enzyme was related to plant response to pathogen inoculation and was higher in inoculated plants of resistant genotype.

Keywords: Black Sigatoka, hydrolytic enzymes, plant defence response, PR proteins
Basic aspects of in vitro organogenesis

Basic aspects of in vitro organogenesis

Authors: Maité Chávez, Manuel de Feria
Year: 2012, Volume: 12, Number: 3
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Abstract

Plant regeneration in vitro of Pinus genus by somatic embryogenesis has been long studied. However, the in v itro establishment of plants from apical shoots and in vitro multiplication of these shoots have been an alternative less studied. Therefore the production of plants, regenerated by organogenesis, able to survive in the acclimatization phase, has been limited. This work aims to present a review of the literature about the in vitro propagation by organogenesis of the genus Pinus, including an analysis of the main factors that could, somehow, affect the success of plant regeneration by this method with emphasis on in vitro and ex vitro root formati on.

Keywords: apical shoots, ex vitro rooting, in vitro rooting, plants regeneration, pine.
Risk assessment of Horizontal Gene Transfer in bacterial contaminants of sugarcane in vitro cultures

Risk assessment of Horizontal Gene Transfer in bacterial contaminants of sugarcane in vitro cultures

Authors: Leonardo J . Moreno-Bermúdez, José Machado-Rodríguez, Yelenys Alvarado-Capó, Mileidy Cruz- Martín
Year: 2012, Volume: 12, Number: 2
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Abstract

The horizontal gene transfer is a phenomenon that must be taken into account when performing risk evaluation of genetically modified plants, because these ones could transmit by this method the artificially incorporated transgenes to other organisms with those they are in strait contact in the environment and affect them somehow. The aim of this study was to evaluate the risks of horizontal gene transfer in bacterial contaminant species of in v it r o culture of non-transgenic sugarcane (Saccharum spp. hybrid), which in some cases coincide with endophytic species and/or residents of the rhizosphere of transgenic plants. It was determined the capacity of natural transformation of fourteen bacterial strains with a plasmid containing genes that confer resistance to antibiotics Ampicillin, Streptomycin and Spectinomycin, used as a vector for plant genetic transformation, and from the calculation of transformation frequencies of each species the risks were assessed. Transformed and non transformed bacteria were obtained. The lower frequency was obtained for Escherichia coli (0.09 x 10 -9 ) and the highest for Pseudomonas stutzeri (266.6 x 10 -9 ). The fact that some of the studied bacteria were naturally transformable is a risk factor for them, and it can be used as a model to study horizontal gene transfer, in case to be found as endophytic or associated with the rhizosphere of plants that have been genetically modified when conducting risks assessments to such plants.

Keywords: Horizontal Gene Transfer, risks evaluation, Saccharum spp. híbrido
Plant  growth  regulators  in  horticulture:  practices  and perspectives

Plant growth regulators in horticulture: practices and perspectives

Authors: João Paulo Tadeu Dias
Year: 2019, Volume: 19, Number: 1
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Abstract

The plant growth regulators (PGRs) modulate plant growth and development and mediate responses to both biotic and abiotic stresses. This paper aims to expose, reflect and discuss about the practical use of PGRs in horticulture and their perspectives. The PGRs are used commercially in agriculture. Besides, in plant tissue culture is traditional practice and have relevant importance. The approach of the application of PGRs in horticulture shows good results. However, it needs more studies, discussions on the subject, which leads to conclude that it use in horticultural plants can be a challenge for all that act in the area, presenting practical advantages and favorable perspectives for future use.

Keywords: development, hormones, plant physiology, vegetables
Potato purple top, Lethal wilt of oil palm, and Papaya twisted neck syndrome: Phytoplasma-associated diseases in Ecuador

Potato purple top, Lethal wilt of oil palm, and Papaya twisted neck syndrome: Phytoplasma-associated diseases in Ecuador

Authors: Carlos Bolanos , Patricio Gallegos, Jose B Ochoa, Maria Insuasti , Veronica Bonilla, Jorge Rivadeneira, Paul Comina, Xavier Cuesta
Year: 2019, Volume: 19, Number: 1
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Abstract

Phytoplasma are wall-less bacteria limited to the phloem vessels in higher plants. Diseases associated with phytoplasma, in the past, have not been a serious problem in Ecuador. Nevertheless, and for climate change effects, their importance has been increasing suddenly. This research was focused on the detection of phytoplasma using nested Polymerase Chain Reaction (PCR), in potato (Solanum tuberosum L.), oil palm (Elaeis guineensis Jacq.), and papaya (Carica papaya L.) plants. Phytoplasma-specific PCR amplifications were generated by nested PCR in 50% samples of twisted neck syndrome in papaya, 38% potato samples showing purple top symptoms, and in all the samples showing symptoms of lethal wilt of oil palm. A continual increase in the incidence of potato purple top was observed, and there is a high risk of contamination in the southern production zones of the country. In potato, results of this study are more closely related to Candidatus Phytoplasma subgroup 16SrI.Lethal wilt continues to be a major threat to oil palm production. In papaya, members of the group 16SrXIII-E produce a disease similar as reported in this study.

Keywords: Candidatus phytoplasma, crop production, oil palm, nested PCR
Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse

Effect of selenite and inulin on the Capsicum annuum L. - Phytophthora capsici interaction in greenhouse

Authors: Julio César López-Velázquez, Diego Eloyr Navarro-López, Joaquín Alejandro Qui-Zapata, Janet María León-Morales, David Ismael Saavedra-Loera, Soledad G...
Year: 2019, Volume: 19, Number: 1
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Abstract

Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on the incidence of Phytophthora capsici and the growth of serrano pepper plants (Capsicum annuum L.) in greenhouse. Seedlings 30 days after sowing were treated twice with selenite (100 μM) (Se + PHC) or inulin (200 μM) (I + PHC) per application to the base of the plants: 10 days before and at the time of inoculation with P. capsici (PHC). A PHC control and an absolute control without treatment or inoculation were included. After two weeks of treatment, the control seedlings remained without symptoms, while those inoculated with PHC, and those treated with selenite and inulin and inoculated showed a survival rate of 0, 80 and 40%, respectively. The height was higher in the control seedlings. In plants treated with selenite and inoculated a seedling fresh mass was higher than in the PHC and I + PHC, treatments. The viability of the root was similar in the treatments with selenite and inulin and decreased in the seedlings inoculated with PHC. Selenite was more effective than inulin in reducing the incidence of the disease, increasing the fresh mass and maintaining the viability of the plant tissue. The results suggest the potential use of selenite and inulin in the integral management of P. capsici.

Keywords: incidence, fructans, plant protection, selenium, symptoms of wilt
Influence of maltose and sucrose on somatic embryos maturation of Glycine max cv. 'Incasoy-27'

Influence of maltose and sucrose on somatic embryos maturation of Glycine max cv. 'Incasoy-27'

Authors: Jorge Pérez Pérez, Lourdes R García, Novisel Veitía, Idalmis Bermúdez-Caraballoso, Raúl Collado, Berkis Roque
Year: 2019, Volume: 19, Number: 1
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Abstract

The somatic embryogenesis in soybean crop has as limitation the low percentage of conversion to plant that is obtained, attributed to the presence of somatic embryos with abnormal morphologies during the maturation phase. This work aim was to determine the influence of maltose and sucrose on somatic embryos maturation of soybean cultivar 'Incasoy-27'. A culture medium containing 6.0% maltose or sucrose and activated carbon as carbon sources was used. The embryos were placed in sunlight growth chamber for eight weeks. The higher percentage (29.75%) of mature somatic embryos with dicotyledonal morphology was achieved when maltose was used. The use of sucrose increased the number of somatic embryos that showed abnormal morphologies as trumpet shape, fasciated, fused, rudimentary cotyledon and long hypocotyl type. These results indicated that carbon source influences the maturation of soybean somatic embryos cultivar 'Incasoy-27'. The somatic embryos cultivated in maltose, increase the morphologies dicotyledonal shape, monocotyledonal and polycotyledonal type, with development of apical and radical meristems.

Keywords: in vitro, osmotic, regeneration, soy, tissue culture
In vitro conservation of Ipomoea batatas (L.) Lam cultivars by minimum growth with the use of mannitol

In vitro conservation of Ipomoea batatas (L.) Lam cultivars by minimum growth with the use of mannitol

Authors: Aymé Rayas Cabrera, Jorge López Torres, Víctor R Medero Vega, Milagros Basail Pérez, Arletys Santos Pino, Yenisey Gutiérrez Sánchez
Year: 2019, Volume: 19, Number: 1
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Abstract

In vitro conservation of Ipomoea batatas (L.) Lam allows the exchange of germplasm and its availability for breeding programs. The objective of this work was to determine the effect of mannitol and abscisic acid in the conservation through minimum growth of I. batatas cultivars from INIVIT Germplasm Bank. Treatments included abscisic acid (ABA) (5 and 10 mg l-1) and mannitol (1.0, 1.5 and 2.0%) in a basal MS culture medium, the combination of ABA (10 mg l-1) and mannitol (1.0 , 1.5 and 2.0%). As controls were used the MS basal culture medium and MS with sorbitol (1.0%) and glucose (1.0%). In the cultivars 'Cautillo' and 'INIVIT BS 16-2006' the best results of survival, growth decrease and green but small leaves were obtained in the treatments that contained the basal culture medium with mannitol (10, 15 and 20%) . Growth of the explants was not achieved in culture media with abscisic acid. The MS culture medium with 2 mg l-1 of thiamine, 100 mg l-1 of myo-inositol and 1.0% of mannitol, allows the in vitro conservation of sweet potato cultivars between six and eight months.

Keywords: osmotic agent, sweet potato, germplasm, culture medium
Micropropagation of Stevia rebaudiana Bertoni plants from ex vitro explants

Micropropagation of Stevia rebaudiana Bertoni plants from ex vitro explants

Authors: Dionys González-Hernández, Elizabeth Kairuz Hernández-Díaz, Alina Capote, Anabel Pérez, Leonardo Rivero, Borys Chong-Pérez, Naivy Pérez-Alonso
Year: 2019, Volume: 19, Number: 1
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Abstract

Stevia rebaudiana Bertoni is a species recognized worldwide for its medicinal properties. The objective of this work was to micropropagate Stevia plants from ex vitro explants. Shoots of plants of this specie growing at greenhouse were selected. In the in vitro establishment, the effect of disinfection time and the concentration of sodium hypochlorite on the survival of the explant were evaluated. The established explants were transferred to the multiplication culture medium, where the effect of the growth regulators (6-benzylaminopurine and indoleacetic acid) and the number of subcultures on the multiplication coefficient were determined. Then, the response of the plants in the acclimatization phase was evaluated, in comparison with plants propagated by cutting. Sodium hypochlorite at 1% allowed the disinfection of the explants with a survival of 94.5%, regardless of the time. The growth regulators did not modify the multiplication coefficient with subcultures every 15 days. It is possible to use different combinations of substrate based on zeolite and compost during the acclimatization of micropropagated plants. These developed a greater number of leaves and height than those propagated by cutting.

Keywords: acclimatization, growth regulators, multiplication procedure, rebaudiosides, steviosides
In vitro phosphate solubilization by Aspergillus and Penicillium strains and coffee plants growth promotion

In vitro phosphate solubilization by Aspergillus and Penicillium strains and coffee plants growth promotion

Authors: Mayra Acosta-Suárez, Mileidy Cruz-Martín, Yelenys Alvarado-Capó, Raul Barbón, Tatiana Pichardo, Eloísa Rodríguez, Alina Capote, Anabel Pérez
Year: 2019, Volume: 19, Number: 1
(1 downloads)
Abstract

The availability of phosphorus in the soil for the growth of plants can be increased by the action of phosphate solubilizing microorganisms. The objective of this work was to determine the potential of Aspergillus and Penicillium strains, solubilizers of phosphates in vitro, to promote the growth of coffee plants obtained by somatic embryogenesis. In vitro tests were carried out with strains of Aspergillus and Penicillium grown in Pikosvkaya culture medium (PVK) to determine their solubilization capacity of phosphates. In addition, PVK and NBRIP liquid where the pH was measured were inoculated in culture medium. Two strains were selected and cultured for 72 h in PVK culture medium. Subsequently, suspensions of conidia in the culture filtrates were applied to the substrate (soil: compost) of Coffea canephora cv. 'Red Caturra' obtained by somatic embryogenesis. After 90 days of culture the height of the plants was measured (cm), the number of leaf pairs was quantified and the dry mass (g) of the biomass was determined. All strains tested showed translucent halos around the colonies in PVK culture medium indicative of phosphate solubilization and reduced pH in liquid culture media. The inoculation of Aspegillus sp. CCIBP-Asp 32 significantly increased the height of the plants and the biomass. The potential of these strains was demonstrated for the formulation of bioproducts that can be applied in green house or nursery to promote the growth of coffee plants in vitro obtained.

Keywords: Coffea arabiga, Pikosvkaya culture medium, phosphorus

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