Medical Laboratory Journal

Medical Laboratory Journal

Basic info

  • Publisher: Deputy of Research and Technology, Golestan University of Medical Science
  • Country of publisher: iran, islamic republic of
  • Date added to EuroPub: 2019/Sep/29

Subject and more

  • LCC Subject Category: Medicine, Clinical Trials, Pathology
  • Publisher's keywords: Medicine, Anatomic Pathology, Clinical Pathology, Hematology, Urinalysis
  • Language of fulltext: english
  • Time from submission to publication: weeks

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  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? No

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  • License terms
  • Open Access Statement: No
  • Year open access content began: 2007
  • Does the author retain unrestricted copyright? False
  • Does the author retain publishing rights? False

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This journal has '48' articles

Evaluation of Relationship between Serum Levels of Inflammatory Factors and Clinical Symptoms in Females with Knee Osteoarthritis

Evaluation of Relationship between Serum Levels of Inflammatory Factors and Clinical Symptoms in Females with Knee Osteoarthritis

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Osteoarthritis (OS) is the most common type of arthritis and joint disease, especially in women. Proinflammatory cytokines, biochemical factors, specially matrix metalloproteinases, and reactive oxygen species play important roles in joint destruction in this disease. Therefore, the present study aimed to evaluate level of inflammatory factors and its relationship with clinical symptoms of OS in female patients. Methods: The study was performed on female patients with knee OS, referring to healthcare centers of Tabriz University of Medical Sciences. After measuring the weight and height of patients, clinical symptoms such as severity of pain and physical performance were evaluated using the Knee Injury and Osteopaedic Outcome Score questionnaire. Serum levels of IL-1β, TNF-α, and hs-CRP in fasting blood samples were measured using ELISA kits and immunoturbidimetric assays. Results: There was a significant association between level of IL-1β and score of pain. There was no significant relationship between the clinical symptoms and level of other inflammatory factors. Conclusion: The results of the present study showed that the increase in inflammatory factors is correlated with severity of pain in OS patients.

Keywords: Osteoarthritis Knee, Female, Inflammatory Markers
Investigation of Frequency of Herpes Simplex Virus in Patients with Type 2 Diabetes and Healthy Individuals by PCR and ELISA

Investigation of Frequency of Herpes Simplex Virus in Patients with Type 2 Diabetes and Healthy Individuals by PCR and ELISA

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Previous studies have demonstrated the relationship between viral infections and risk of developing type 1 diabetes. The aim of this study was to investigate the frequency of Herpes simplex virus (HSV) in patients with type 2 diabetes and healthy control individuals using PCR and ELISA. Methods: Blood samples were taken from 180 diabetic patients and 187 healthy controls referred to the Pasteur medical laboratory in Tonekabon, in 2016. Human beta-globin gene was used as internal control to ensure extraction accuracy. Specific primers were used for amplification of the UL30 gene. In addition, level of anti-HSV IgG antibody was measured using a commercial ELISA kit (Euroimmun, Germany). Results: DNA of HSV was found in the samples of 11 patients (6.1%) and five healthy controls (2.7%). In addition, anti-HSV IgG was found in the samples of 117 patients (65%) and 108 healthy controls (57.75%). There was a statistically significant relationship between frequency of anti-HSV IgG and diabetes. Conclusion: Similar to previous studies, the present study demonstrated a relationship between frequency of HSV infection and type 2 diabetes. However, further studies should be performed to eliminate the effect of other risk factors to help clarify the exact role of viral infections in increasing the risk of diabetes. Keywords: Diabetes, Herpes Simplex Virus, ELISA, PCR.

Keywords:
Effect of Vigorous Aerobic Exercise on Serum Levels of SIRT1, FGF21 and Fetuin A in Women with Type II Diabetes

Effect of Vigorous Aerobic Exercise on Serum Levels of SIRT1, FGF21 and Fetuin A in Women with Type II Diabetes

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and Objectives: Sirtuin (SIRT), Fibroblast Growth Factor21 (FGF21) and Fetuin A are proteins that cause a wide range of metabolic disorders such as type 2 diabetes mellitus (T2DM). On the other hand, regular physical activity is known to play a key role in prevention and management of T2DM. Thus, this study investigated the effect of vigorous aerobic exercise on serum levels of metabolic parameters including SIRT1, FGF21 and Fetuin A in women with T2DM. Methods: The study was performed on 28 randomly selected women with T2DM who were divided into an exercise group and a control group. The training intervention consisted eight weeks of vigorous aerobic exercise (three times a week at 70-80% of maximum heart rate). The serum levels of SIRT1, FGF21 and Fetuin A were evaluated before the first session and 48 hours after the last session. Paired sample t-test and independent t-test were used to analyze within and between group differences, respectively. All statistical analyses were performed in SPSS (version 19) at significance of 0.05. Results: The eight-week aerobic training caused a significant reduction in body weight, body mass index, insulin resistance, low-density lipoprotein, fasting blood sugar, triglycerides and Fetuin A of women with T2DM. In addition, it caused a significant increase in SIRT1 and FGF21 levels. There was no significant difference in the level of high-density lipoprotein and cholesterol between the two groups. Conclusion: As a non-pharmacological therapy, regular aerobic exercise might improve the metabolic parameters, SIRT1, FGF21, and Fetuin A in women with T2DM. Keywords: Diabetes Mellitus Type 2, SIRT1, FGF21, Fetuin A, Exercise.

Keywords: Diabetes Mellitus Type 2, SIRT1, FGF21, Fetuin A, Exercise.
Effect of 12 Weeks of Intense Endurance Training and Bee Pollen Consumption on ABCA1 Gene Expression in Small Intestine, Liver and Gastrocnemius Muscle of Male Rats

Effect of 12 Weeks of Intense Endurance Training and Bee Pollen Consumption on ABCA1 Gene Expression in Small Intestine, Liver and Gastrocnemius Muscle of Male Rats

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: The aim of this study was to evaluate the effect of 12 weeks of intense endurance training and bee pollen consumption on ABCA1 gene expression in small intestine, liver and gastrocnemius muscle tissues of male rats. Methods: In this study, 24 male Wistar rats (aged 6-8 weeks and weighing 90-110 g) were randomly divided into four groups of saline-control (n=6), saline-training (n=6), bee pollen-control (n=6) and bee pollen-training (n=6). The training groups exercised on a treadmill for 12 weeks (30 m/min, 90 min/day, five days/week). The bee pollen groups were given bee pollen orally (500 mg/Kg) for 12 weeks. Data were analyzed using two-way ANOVA at significance level of 0.05. Results: ABCA1 gene expression was highest in the liver, gastrocnemius muscle and small intestine, respectively. The findings also revealed that the intense endurance training caused a non-significant increase in ABCA1 gene expression in the small intestine and liver. However, the training caused a non-significant decrease in ABCA1 gene expression in the gastrocnemius muscle. In addition, consumption of bee pollen significantly increased ABCA1 gene expression in the small intestine and gastrocnemius muscle of male rats. However, the effect of bee pollen on the gene’s expression in the liver was not statistically significant. Conclusion: Based on our findings, it can be concluded that consumption of bee pollen has more beneficial effects on the ABCA1 gene expression and reverse cholesterol transport compared with the intense endurance training.

Keywords: ABCA1 protein, Pollen, exercise
Effect of Intra-Uterine Injection of hCG on Implantation Rate in IVF Candidates

Effect of Intra-Uterine Injection of hCG on Implantation Rate in IVF Candidates

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Human chorionic gonadotropin (hCG) is the embryonic hormone and one of the most important factors for implantation. The present study aimed to evaluate effect of intra-uterine injection of hCG before embryo transfer on the pregnancy outcome in IVF. Methods: In this study, 180 infertile patients were randomly allocated into an experiment and a control group. In the experiment group, before embryo transfer, 500 IU hCG were injected into the endometrium cavity in puncture stage. Equal amount of phosphate buffer (placebo) was injected to the subjects in the control group. Pregnancy test was done two weeks after the intervention. In case of positive results, ultrasound scan was done for detecting the gestational sac and numbers of sacs. Results: Mean age of the patients was 30±3.41 years and 31±4.12 years in the experimental and control group, respectively. There was no statistically significant difference in the duration of infertility between the two groups (4.77±3.67 years vs. 4.87±3.74 years). Based on the hCG level, the pregnancy rate was 35.5% in both groups. However, based on ultrasound findings, the pregnancy rate was 35.5% and 28.8% in the experiment group and the control group, respectively (P=0.33). In addition, there was no significant difference in the pregnancy rates between different age groups. Conclusion: Our results indicate that the intra-uterine injection of β-hCG has no significant effect on the pregnancy rate in IVF.

Keywords: Fertilization in Vitro, Chorionic Gonadotropin, Injections.
Frequency and Antibiotic Resistance Pattern of Diarrheagenic Escherichia coli (DEC) Strains Isolated from Children Aged Less Than 10 Years

Frequency and Antibiotic Resistance Pattern of Diarrheagenic Escherichia coli (DEC) Strains Isolated from Children Aged Less Than 10 Years

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and Objectives: Diarrheagenic Escherichia coli (DEC) pathotypes are important causes of diarrhea among children in developing countries. The objective of this study was to determine the frequency and antibiotic resistance pattern of DEC pathotypes in children aged less than 10 years. Methods: This cross-sectional study was done on 300 E. coli strains isolated from diarrheic stool samples of children aged less than 10 years who were admitted to hospitals and central laboratory in Zahedan, between July and October 2016. DEC pathotypes were identified by standard biochemical testing and phenotypic testing using polyvalent antiserums. Antibiotic resistant pattern of these strains was evaluated against 11 different antibiotics by the agar disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines. Results: Of the 300 E. coli isolates, 89 (29.6%) were found positive for DEC using polyvalent antiserums. In this study, 35 cases (39.3%) reacted with antiserum 1, 21 cases (25.8%) reacted with the antiserum 2, and 31 cases (34.8%) reacted with antiserum 3. The highest rate of resistance was observed against ampicillin (94.8%), tetracycline (87.2%), and co-trimoxazole (70.5%). In addition, the lowest rate of resistance was related to imipenem (1%) and ciprofloxacin (8.9%). Conclusion: DEC pathotypes are the important causes of diarrhea among children admitted to hospitals of Zahedan. Considering the high rate of antibiotic resistance among these pathotypes in this region, prescription of antibiotics should be based on accurate detection of these strains. Keywords: Escherichia coli, Child, Antibiotic Resistance.

Keywords: Escherichia coli, Child, Antibiotic Resistance.
Antibiotic Resistance Patterns and Prevalence of PER and VEB Resistance Genes among Clinical Isolates of ESBL-Producing Acinetobacter Baumannii

Antibiotic Resistance Patterns and Prevalence of PER and VEB Resistance Genes among Clinical Isolates of ESBL-Producing Acinetobacter Baumannii

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Acinetobacter species are responsible for a wide range of clinical complications in hospitalized patients. Antimicrobial treatment of clinical strains of Acinetobacter baumannii may be compromised due to multiple-drug resistance to b-lactams. Aim of this study was to determine antibiotic resistance patterns and frequency of PER and VEB genes in A. baumannii isolates from hospitalized patients. Methods: In this cross-sectional study, 100 clinical strains of A. baumannii were isolated from patients hospitalized in Qom (Iran) using specific culture media and biochemical tests. The disk diffusion method was performed to determine resistance to some antibiotics. Minimum inhibitory concentration (MIC) for cefepime and ceftazidime was evaluated. Identification of ESBL-producing strains and presence of the PER and VEB genes were determined by combined disk test and polymerase chain reaction, respectively. Results: The isolates were highly resistant against cefixime, ceftriaxone and cefepime. Lowest level of resistance was against polymyxin B. In addition, 70% of the isolates were multi-drug resistant. MIC<128 µg/ml to ceftazidime and cefepime was observed in 84% and 91% of the strains, respectively. Moreover, 21% of the strains were ESBL-positive and frequency of the PER and VEB genes was 47% and 32%, respectively. Conclusion: Majority of A. baumannii isolates are highly resistant to the tested antibiotics. Due to presence of the PER and VEB genes in the isolated strains, there is the possibility of resistance spread to other bacteria. Therefore, it is recommended to modify the consumption pattern for antibiotics and pay more attention to standards of nosocomial infection control.

Keywords: Acinetobacter baumannii, Drug resistance, PER, VEB
Evaluation of Antibiotic Resistance Patterns and Frequency of Carbapenemase-Producing Acinetobacter baumannii Isolates by the Carbacineto NP Test

Evaluation of Antibiotic Resistance Patterns and Frequency of Carbapenemase-Producing Acinetobacter baumannii Isolates by the Carbacineto NP Test

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and Objectives: Acinetobacter baumannii is an opportunistic pathogen associated with nosocomial infections. Treatment of infections caused by this bacterium has become challenging due to increasing rate of resistance to a wide range of antibiotics such as carbapenems. One of the main mechanisms of resistance to carbapenems is the production of carbapenemase. The objective of this study was to evaluate antibiotic resistance patterns and frequency of carbapenemase-producing A. baumannii strains using the CarbAcineto NP Test. Methods: In this descriptive cross-sectional study, 130 A. baumannii isolates were collected from clinical specimens of teaching hospitals in Zahedan in 2016. After determining the antibiotic resistance patterns, all A. baumannii isolates were examined using the phenotypic method of CarbAcineto NP test to evaluate production of carbapenemase enzymes. Results: Based on the antibiogram results, more than 90% of the isolates were resistant to the antibiotics tested in this study. However, the lowest rate of resistance was observed against colistin, minocycline, tigecycline and doxycycline, respectively. Based on the results of the CarbAcineto NP test, 96% of carbapenem-resistant strains were positive for the production of carbapenemases. Conclusion: Due to the high resistance of A. baumannii to carbapenems, they are not currently suitable for the treatment of infections caused by this bacterium. However, since most carbapenem-resistant strains are susceptible to colistin, minocycline, tigecycline, and doxycycline, these antibiotics or their combination are recommended for the treatment of the infections caused by the resistant strains. Rapid identification of carbapenemase-producing bacteria using efficient methods such as CarbAcineto NP test is essential to prevent their spread, particularly in hospitals. KEYWORDS: Acinetobacter baumannii, Carbapenemase, CarbAcinetoNP Test.

Keywords: Acinetobacter baumannii, Carbapenemase, CarbAcinetoNP Test.
Clinical Evaluation of the Tonsils for Malignancy: The Necessity of Histopathological Examination

Clinical Evaluation of the Tonsils for Malignancy: The Necessity of Histopathological Examination

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Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Tonsils are lymphoid tissues that can be affected in various diseases. In some cases, it is necessary to remove tonsils through surgery. Although the diseases are often diagnosed by history-taking and physical examination that proceeds with surgery, histologic examination is necessary for confirmed diagnosis of malignancy. Considering the low prevalence of tonsil cancer, this study was performed to clinically examine tonsils for malignancies and evaluate the necessity of histopathological examination. Methods: This retrospective study was conducted on 2,232 patients (aged 2-80 years) who had undergone tonsillectomy for various reasons in the city of Gorgan from 2001 to 2012. Medical history was obtained and physical examination was carried out before surgery. All the obtained samples were sent to the laboratory for histopathological examination. Finally, the collected data was analyzed. Results: Only one case of lymphoma was found among the 2,232 patients investigated in this study. This 80-year-old woman with preoperative cervical lymphadenopathy and unilateral tonsillar enlargement went under tonsillectomy for confirmed diagnosis. Conclusion: Considering the high frequency of tonsillectomy and the low possibility of malignancy, and the fact that malignancy is often seen in individuals with obvious clinical presentations such as in our case, it is recommended to limit referral of specimens for histological examination to highly suspicious cases through more rational decision-making and summarization of other findings.

Keywords: Hypertrophy, Malignancy, Lymphoma Pathology, Tonsillectomy
Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori

Inhibitory Effect of Probiotic Bacteria Isolated from Camel Milk on Clinical Strains of Drug-Resistant Helicobacter pylori

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and Objectives: Helicobacter pylori is the most common cause of gastritis and ulcer worldwide. Treatment of such infections may lead to failure due to drug resistance. This study aimed to investigate the antimicrobial effects of bacteria present in camel milk on the growth of drug-resistant clinical isolates of H. pylori. Methods: In this cross-sectional study, biopsy samples from 75 patients with digestive symptoms were transferred to laboratory in transport medium containing homogeneous compounds. In order to isolate H. pylori, urease-positive biopsies were promptly cultured in brucella agar enriched with defibrinated sheep blood and fetal calf serum. Disk diffusion agar test was used to evaluate antibiotic susceptibility and agar well diffusion method was applied to study the antagonistic effect of probiotics isolated from camel milk on the H. pylori isolates. Results: The frequency of H. pylori isolates was 42.7%. The highest rate of resistance was observed against metronidazole (56.3%). In addition, the rate of resistant to amoxicillin, ciprofloxacin, and clarithromycin and tetracycline was 31.3%, 18.8%, 15.6%, respectively. Lactobacillus plantarum (59.3%) was more frequent than other Lactobacillus species. L. plantarum, Lactobacillus fermentum and Lactobacillus casei showed favorable inhibitory effects against the H. pylori isolates, but L. plantarum (with inhibition zone diameter of 20.3 mm) showed the highest inhibitory effect. Conclusion: Considering the increasing rate of drug resistance and the inhibitory effect of probiotics isolated from milk, health providers recommend that promoting consumption of probiotic food seems beneficial for the general population and those suffering from gastrointestinal disorders. Keywords: Helicobacter pylori, Drug resistance, Camel, milk, Probiotics.

Keywords: Helicobacter pylori, Drug resistance, Camel, milk, Probiotics.
Effects of Resistance Training with and without Blood Flow Restriction on Cardiovascular Risk Factors in Overweight Females

Effects of Resistance Training with and without Blood Flow Restriction on Cardiovascular Risk Factors in Overweight Females

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

Background and Objectives: Elevated blood lipid levels are correlated with risk of cardiovascular disease, especially in overweight individuals. The beneficial effect of strength training on lipid profile has been well documented. However, the effect of strength training with flow blood restriction has not been established. The purpose of this study was to compare the effect of eight weeks of strength training with and without blood flow restriction on some of the cardiovascular risk factors in overweight females. Methods: In this study, 28 overweight females aged 18 to 24 years were randomly assigned into three groups of blood flow restriction strength training (BFRT), traditional strength training (TRT) and control. Participants in the BFRT group performed eight weeks of strength training three sessions per week at 30% of 1-repetetion maximum, while participants in the TRT group performed the training at 65-80% of 1-repetetion maximum. Cardiovascular variables, systolic blood pressure and diastolic blood pressure were measured before and after the exercise training. Data was analyzed using descriptive statistics, paired t-test and one-way analysis of variance. Results: Triglyceride level decreased significantly in the BFRT (p=0.022) and TRT (p=0.038) groups compared with the control group. Moreover, there were no significant differences in the body mass, waist-to-hip ratio, body mass index, high-density lipoprotein, low-density lipoprotein, and cholesterol levels between the study groups (P≥0.05). Conclusion: Our findings suggest that the BFRT and TRT may not be suitable for preventing or even reversing the physiological changes induced by obesity.

Keywords: Resistance Training, Lipids, lipoprotein, overweight
Effect of Eight Weeks of Cardiac Rehabilitation Training on PPAR-α Gene Expression in CABG Patients

Effect of Eight Weeks of Cardiac Rehabilitation Training on PPAR-α Gene Expression in CABG Patients

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and objectives: Cardiovascular disease is the leading cause of death worldwide. This study examined the effects of cardiac rehabilitation exercise on lipid profile and expression of peroxisome proliferator-activated receptor alpha (PPAR-α) gene in patients who underwent coronary artery bypass grafting. Methods: In this quasi-experimental study, after screening, patients who underwent coronary artery bypass grafting (CABG) were randomly divided into an experimental group (n=12) and a control group (n=12). After the surgery and discharge from hospital, the experimental group performed rehabilitation exercise for two months, while the control group did not perform any exercise after discharge from the hospital and the initial phase of rehabilitation. Fasting blood samples were collected before and after the last training session to evaluate biochemical variables and PPAR-α gene expression of lymphocytes. PPAR-α expression level was assessed by qRT-PCR. Statistical analysis was done in the SPSS software (version 20) using repeated measures. Results: In the follow-up after the two-month cardiac rehabilitation exercise, the PPAR-α gene was significantly overexpressed and plasma HDL levels increased significantly in the training group compared with the control group (P<0.05). Although the concentrations of LDL and triglycerides decreased in the experimental group, this reduction was not statistically significant (P>0.05). Conclusion: The results indicate that the protocols carried out in the study could be utilized for improving HDL levels and cardiovascular function in CABG patients. keywords: Cardiac Rehabilitation, Gene Expression, PPAR-α.

Keywords: Cardiac Rehabilitation, Gene Expression, PPAR-α.
Effect of Eight Weeks of Aerobic Exercise on Iron Status and Coagulation and Immune Indices in Non-Athlete Females

Effect of Eight Weeks of Aerobic Exercise on Iron Status and Coagulation and Immune Indices in Non-Athlete Females

Authors:
Year: 2018, Volume: 12, Number: 1
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Abstract

ABSTRACTBackground and objectives: The aim of this study was to investigate the effect of eight weeks of aerobic exercise on serum iron, serum ferritin, platelet count, mean platelet volume, and some immunity indices in non-athlete females.Methods:In this study, 30 non-athlete females aged 30-45 years were randomly divided into an experimental and a control group. The experimental group was subjected to perform aerobic exercise at 60-70% of maximal heartrate for eight weeks, three sessions per week and one hour per session. Blood parameters were measured before the exercise program and after the completion of program by taking 2 ml blood samples from the venous vein of the participants in the sitting position. Finally, t-test was used to compare the pre-test and post-test means of each group and independent t-test was used to compare the two groups at significance of 0.05.Results: After eight weeks of aerobic exercise, there was a significant increase in mean platelet volume and a significant decrease in serum iron and ferritin levels in the experimental group (P <0.05). There was a slight decrease in the number of platelets, white blood cells, granulocytes, monocytes and lymphocytes (P<0.05).Conclusion: We recommend physical education instructors, athletes and non-athletes to consider iron supplementation during aerobic exercise, and modify appropriate training intensity based on the fitness and age of trainees to prevent risk of anemia

Keywords: Aerobic exercise, platelet, iron, ferritin, white blood cell
Effect of Six Months of Aerobic Exercise on Lipid Profile, Inflammatory Markers and Risk Factors of Cardiovascular Disease in Obese Women

Effect of Six Months of Aerobic Exercise on Lipid Profile, Inflammatory Markers and Risk Factors of Cardiovascular Disease in Obese Women

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and objectives: The aim of this study was to investigate the effects of six months of aerobic exercise on lipid profile, inflammatory markers, and risk factors of cardiovascular disease in middle-aged obese women. Methods: Fifteen obese woman (age range: 35 to 45 years) with BMI of ≥30 Kg/m2 were enrolled via purposeful and convenience sampling. The participants were randomly divided into training (n=10) and control (n=5) groups. Blood samples (7 ml) were taken from all participants before the first training session and after the last training session. The women performed 60 minutes of aerobic exercise at 55-65% of heart rate reserve, three sessions a week, for six months. Data was analyzed using independent and paired t-tests and Mann-Whitney U and Wilcoxon tests at significance of 0.05. Results: After the six-month aerobic exercise, C-reactive protein, low-density lipoprotein, triglycerides, total cholesterol and high-density lipoprotein/total cholesterol decreased significantly. The exercise intervention also caused a significant increase in the concentrations of high-density lipoprotein and apolipoprotein A. Conclusion: Aerobic exercise can reduce the level of inflammatory markers associated with risk of cardiovascular disease and atherosclerosis. Keywords: Lipid profile, Inflammatory markers, Obese women, Apolipoprotein, Aerobic exercise.

Keywords: Lipid profile, Inflammatory markers, Obese women, Apolipoprotein, Aerobic exercise.
Evaluation of CCR5Δ32 Polymorphism in Patients with Systemic Lupus Erythematosus and Healthy Individuals

Evaluation of CCR5Δ32 Polymorphism in Patients with Systemic Lupus Erythematosus and Healthy Individuals

Authors:
Year: 2018, Volume: 12, Number: 2
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Abstract

ABSTRACT Background and Objectives: C-C chemokine receptor type 5 (CCR5) is a chemokine receptor expressed at high levels on the surface of T-cells. A 32-bp deletion in the coding region of the CCR5 (CCR5Δ32) leads to production of an incomplete protein that is not expressed on the cell surface. CCR5Δ32 may be involved in development of autoimmune disease, such as systemic lupus erythematosus. We investigated frequency of the CCR5Δ32 polymorphism in SLE patients and healthy controls, and evaluated the relationship between the CCR5Δ32 polymorphism and susceptibility to SLE in Golestan Province, Iran. Methods: Whole blood samples were taken from 80 SLE patients admitted to Shahid Sayyad Shirazi hospital and 80 healthy controls (from a blood bank) in the Golestan Province, in 2016. Baseline clinical and laboratorial characteristics were evaluated regarding the CCR5Δ32 genotypes. The CCR5Δ32 polymorphism was determined from genomic DNA by polymerase chain reaction. Result: Genotype frequencies of both groups were in the Hardy-Weinberg equilibrium. The frequencies of the CCR5 and the CCR5Δ32 alleles were 98.13% and 1.88% among the patients, and 98.75% and 1.25% among the controls, respectively. Homozygote CCR5Δ32 was not observed in the subjects. The frequency of heterozygous Δ32 was 3.8% and 2.5% among the SLE patients and controls, respectively (P-value>0.05). There was no significant association between the CCR5 status and clinical signs of SLE (P>0.05). Conclusion: Our data suggest that the CCR5Δ32 polymorphism has no correlation with SLE in our study population. In addition, the frequency of the Δ32 polymorphism in SLE patients and controls does not follow the Hardy-Weinberg equilibrium Keywords: CCR5, Homozygote CCR5Δ32, Heterozygote CCR5Δ32, CCR5Δ32 allele, SLE.

Keywords: CCR5, Homozygote CCR5Δ32, Heterozygote CCR5Δ32, CCR5Δ32 allele, SLE.

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