Biogenesis: Jurnal Ilmiah Biologi

Biogenesis: Jurnal Ilmiah Biologi

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  • Publisher: Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Makassar
  • Country of publisher: indonesia
  • Date added to EuroPub: 2019/Sep/29

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  • Year open access content began: 2013
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This journal has '101' articles

Fermentasi Etanol dengan Bahan Baku Produk Sakarifikasi Singkong oleh Aspergillus niger dengan Menggunakan Isolat Saccharomyces spp. (NKB dan NKC)

Fermentasi Etanol dengan Bahan Baku Produk Sakarifikasi Singkong oleh Aspergillus niger dengan Menggunakan Isolat Saccharomyces spp. (NKB dan NKC)

Authors: Titin Herawati, Theresia Tri Suharni
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Abstract

Nowdays the production of petroleum energy resource decreases in every years. Ethanol is one of renewable source of energy, it can substitution of fosil fuel. Ethanol can be produced from fermentation by Saccharomyces. The raw material of ethanol fermentation is carbohydrate rich organic. Cassava is one of efisien based material for ethanol fermentation because of its attainable price and availability. The objectives of this study were to isolation yeast from coconut neera, and use the isolate as microbial etanol fermentation from cassava. The research was started with isolating yeast from coconut neera, and then how to identified the yeast isolates by morphological and physiological characteristic. Then next process was saccharification the cassava by Aspergillus niger. Product of saccharification added by molasses to obtain 10% and 15% reducing sugar content and subjected for ethanol fermentation. The product of fermentation was analysed for the ethanol concentration by the conway microdiffuse and GCMS methode, Reducing sugar concentration was determined by DNS methode, total yeast was determined by spectroscopy methode and pH was determined by pH meter. The result showed that the isolation from coconut neera get 4 yeast isolate Saccharomyces spp. namely NKA, NKB, NKC, and NKD. The NKB and NKC isolates were choosen as candidate being used for ethanol fermentation process. The process saccharification produced 8,43% of reducing sugar. The result of ethanol fermentation process from the product saccharification of cassava and molasse mixture with content 10 % of reducing sugar were: NKB 4,19% and NKC 5,19 %. Whereas the result ethanol fermentation from the product saccharification of cassava and molasse mixture with content 15 % of reducing sugar were: NKB 6,75% and NKC 6,34 %. Based on this study, it capable concluded that mixed of the product saccharification process by cassava and molasse can be used as raw material fermentation process of ethanol by Saccharomyces spp. isolate NKB and NKC which are to isolate from coconut neera.

Keywords: cassava, ethanol fermentation, molasses, saccharification, Saccharomyces spp.
Pertumbuhan Gross dan Net Populasi Ternak Sapi di Sulawesi Selatan

Pertumbuhan Gross dan Net Populasi Ternak Sapi di Sulawesi Selatan

Authors: Basir Paly
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Abstract

This research aims to know the Gross and the Net population growth of beef cattle the last 5 years (2009-2013) in South Sulawesi. Implemented in four regency, namely, Gowa, Bone, Barru and Enrekang. Site selection was based on the consideration that the four regency are the areas of the development of cattle with a total population of about 45.26% of the total population who are of South Sulawesi. Type of this research is quantitative survey method. The population of the research was the whole group/cattle farmers in four areas. Then each regency selected four groups as the sample, so that the number of samples that are observed as many as 16 groups. Data collection was done through observation and interviews by using the questionnaire. The observed variables are; (1) the number and type of livestock owned, (2) the number of males and females, (3) the addition of the parent, males and females from outside the province, (4) sales, cut withholding, and cases of death, and (5) the number of births. The results showed that over the last five years (2009-2013) Gross population growth of beef cattle in South Sulawesi 11.46% per year, growth above the national average of 10.26% per year. While the growth of the Net population average at 11.18% per year, whereas the Net population smaller (11: 18 < 11.46) from the Gross population. Its main cause is the amount of sales, cut withholding, and cases of death which is not compare with birth rate.

Keywords: gross, growth, net, population
Analisis Kualitas Limbah Cair Pada Kolam Anaerob IV di Instalasi Pengolahan Air Limbah (IPAL) PT. Perkebunan Nusantara VII (Persero) Unit Usaha Bekri

Analisis Kualitas Limbah Cair Pada Kolam Anaerob IV di Instalasi Pengolahan Air Limbah (IPAL) PT. Perkebunan Nusantara VII (Persero) Unit Usaha Bekri

Authors: Mahfut Mahfut
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Abstract

Among the industries in Lampung, PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is one of the Palm Oil Processing Plant (POPP) industry who spend a lot of waste water in the form of residual organic liquid materials. The rest of the liquid material can cause problems such as pollution so it needed special treatment before the waste water is discharged into the environment. This study was conducted to determine the quality of liquid waste in an anaerobic pool IV on Wastewater Treatment Plant (WWTP) PT. Plantation Nusantara VII (Persero) Business Unit Bekri so it can be determined whether the waste water treatment process can improve the quality of waste water for the better. Waste water quality measurement is done using the parameters of physics, chemistry, and heavy metals, namely pH, COD, BOD5, TSS, Cd, Cu, Pb, and Zn. The results showed that the quality of the wastewater in an anaerobic pool IV was good and meets the quality standards of palm oil mill effluent by the State Minister of Environment Resolution No. 03 / MENKLH / 11/1991 and the Indonesian Government Regulation No. 20 of 1990 on Water Pollution Control. It also indicates that the effluent treatment system at the Wastewater Treatment Plant (WWTP) PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is very effective.

Keywords: anaerobic pool, liquid waste, PTPN VII
Penggunaan Ampas Batang Pisang Terhadap Pertumbuhan Ikan Mas di Desa Salutambun Barat Kabupaten Mamasa

Penggunaan Ampas Batang Pisang Terhadap Pertumbuhan Ikan Mas di Desa Salutambun Barat Kabupaten Mamasa

Authors: Hasria Alang
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Abstract

The research of the influence of the banana steam residues as pellet for Cyprinus carpio growth has been done at west Salutambun village, Buntu Malangka Districts, Mamasa Counties. The type of this research is experimental study and the aim of this study to see the effect of alternative pellet for Carp (Cyprinus carpio) growth. The research method include the manufacture and testing of alternative pellets for carp (Cyprinus carpio). Alternative pellets consist of banana stem as the main ingredient, a banana peel, fine bran and sweet potato leaves. Everything yielded then mixed with rice water. The pellets are then dried and cured for one night and then milled to from a circle with a small sircle. The data analysis technique used is completely randomized (RAL) is fine that the F value 14,85** is greater than F table α 0,01 with value 13,74 (F hitung > F tabel). Then there is a very significant effect residual use in the manufacture of banana pellets for carp growth at West Salutambun village, Buntu Malangka Districts, Mamasa Counties.

Keywords: Cyprinus carpio (carp), growth, pellets
Pemanfaatan Buah Mangrove Sebagai Sumber Makanan Alternatif di Halmahera Barat, Maluku Utara

Pemanfaatan Buah Mangrove Sebagai Sumber Makanan Alternatif di Halmahera Barat, Maluku Utara

Authors: Jailan Sahil, Irham Soamole
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Abstract

This study aims to determine: (1) how the management of mangrove fruit as alternative food ingredients (2) types of mangrove that use by people as alternative food items. The method used in this research is the development of research that displayed products to be produced and can be implemented. The results showed that the process of making mangrove fruit as food begins with boiling mangrove fruit, stripping, immersion with ash, drying and milling into flour. Furthermore, the starch is used as raw material for making dry or wet cake. The organoleptic test used to determine the level of preference or the viability of a product to be accepted by the panelists (consumers) includes flavor, aroma, texture and color. The 30 respondents answered 4 (preferred) and 5 (strongly preferred). This shows that they like the products from these two types of mangrove.

Keywords: alternative food, mangrove fruit, North Maluku
Isolasi dan Karakterisasi Actinomycetes Sebagai Penghasil Antibiotik Dari Sampel Tanah Pada Peternakan Sapi di Kecamatan Galesong Kabupaten Takalar

Isolasi dan Karakterisasi Actinomycetes Sebagai Penghasil Antibiotik Dari Sampel Tanah Pada Peternakan Sapi di Kecamatan Galesong Kabupaten Takalar

Authors: Adriani Hasyim, Yessica Febriwanti Tulak
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Abstract

A research on the isolation and characteristics of the antibiotic-producing actinomycetes from soil sample on dairy farms in the district Galesong Takalar has been done for obtaining antibiotic-producing actinomycetes isolates. This study is an exploratory study with laboratory. This research method is the agar diffusion method. Isolation was conducted using scratch method, 7x24 hour incubation period. Isolates obtained was purified on a medium Glycerol Yeast Extract Agar (GYEA). Isolates fermented for 7x24 hours on the tube with the intensity of 170 rpm at room temperature. Fermentation result was tested for antibiotic activity. Both isolates were effective as an antibiotic against bacteria used in the test.

Keywords: Actinomycetes, antibiotics, characterization, isolation, soil, Takalar
Pola Hubungan Antara Jumlah Kelahiran Dengan Jumlah Betina dan Pejantan Produktif Pada Ternak Sapi

Pola Hubungan Antara Jumlah Kelahiran Dengan Jumlah Betina dan Pejantan Produktif Pada Ternak Sapi

Authors: Basir Paly
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Abstract

This research aims to know the pattern of the relationship between the number of births and the number of males and females productive. Implemented in the Gowa in June-October 2014. Using method of survey through observation and interview. The population of the research was a whole group of cattle there. As many as 5 group of cattle has been observed and interviewed. Variables are observed and analyzed are: (1) the number of cattle owned by males and females, (2) the number of births, (3) the number of males and females productive, (4) sales, cut withholding, and cases of death, and (5) number of females productive female rescue. Data analysis was carried out in two categories, descriptive and inferensial. Descriptive analysis using a matrix, tabulate and a chart, while inferential analysis using SPSS and Amos 16 software, then validated with data and information from other sources. The results showed that there are four variables that are directly related to the number of births. Namely: (1) the number of female productive, (2) number of females sold, cut withholding, and death, (3) number of productive female rescue, and (4) the number of male productive. To four variables are also interrelated with one another, but not directly related to the number of births. This powerful relationship patterns indicate that the number of births is very closely related to the number of productive female rescue, and the number of males. While the weak relationship patterns indicate that the number of females sold, cut withholding, and the dead, is not related to the number of births.

Keywords: birth, females, males, relationships
Pertumbuhan Tiga Kultivar Padi (Oryza sativa L.) Terhadap Aplikasi Pupuk Bio Cair dan Kondisi Tanah Pertanian Pasca Erupsi Merapi 2010

Pertumbuhan Tiga Kultivar Padi (Oryza sativa L.) Terhadap Aplikasi Pupuk Bio Cair dan Kondisi Tanah Pertanian Pasca Erupsi Merapi 2010

Authors: Dwi Umi Siswanti, Diah Rachmawati
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Abstract

The eruption of Mount Merapi at the end of 2010, which resulted in the destruction of land and crops are changing wetland into dry land. Sleman which is located south of Mount Merapi became one of the areas that most affected by the eruption of Merapi in 2010. It is necessary to improve the processing of soil physical properties of degraded areas and efforts to restore agricultural soil nutrient. Returning soil nutrients can be cultivated with the addition of organic material or the addition of microbes. The research aims to analyze the growth of three cultivars of rice (Oryza sativa L.) against the application of biofertilizer in paddy fields were affected by the eruption of Merapi in 2010. These three rice cultivars are Situbagendit, Inpari 20 and Ciherang. This research was conducted by applying the biofertilizer as much as 10 liters / ha were given three times during the vegetative phase, ie: 7, 21 and 35 days after planting. The results of this research indicate that the three rice varieties ie: Situbagendit, Ciherang and Inpari 20 have an optimal growth and an increase in soil nutient content in biofertilizer application.

Keywords: biofertilizer, degraded land, Merapi eruption, rice
Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH

Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH

Authors: Nurhidayah Nurhidayah, Masriany Musa, Mashuri Masri
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Abstract

This research aims to determine the highest protein content and the optimum activity of the enzyme bromelain extracted from pineapple stem. This research was conducted at the Laboratory of Microbiology Makassar Alauddin State Islamic University in May 2013. Variable in this study was the isolation and measurement of bromelain enzyme activity as the independent variable while the crude extract of pineapple stem by variations in pH as the dependent variable. The type of this research was experiments with bradford method for determination of protein content enzyme bromelain, with ammonium sulfate concentration variation for precipitation was 10-60%, as well as the determination of the enzyme bromelain activity at variation pH was 4.0; 5.0, 6.0; 7.0 and 8.0, at 650C temperature with incubation time was 10 minutes. Each stage has done three times in repetition and analyzed spectrometry. The results showed the highest levels of protein precipitation with ammonium sulfate at 60% was 37,785 mg/ml. While the optimum pH of the enzyme bromelain activity at pH 6.0 was 1.021 activity units /gram.

Keywords: bromelain enzyme, Pineapple stem (Ananas comosus), precipitation with ammonium sulfate, protein contents, pH
Pertumbuhan Tanaman Stroberi Pada Berbagai Jenis dan Konsentrasi Pupuk Organik Cair dan Urine Sapi Dengan Sistem Hidroponik Irigasi Tetes

Pertumbuhan Tanaman Stroberi Pada Berbagai Jenis dan Konsentrasi Pupuk Organik Cair dan Urine Sapi Dengan Sistem Hidroponik Irigasi Tetes

Authors: Nurlailah Mappanganro
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Abstract

The research aimed at investigating the effect of the liquid organic fertilizer from the cattle dung towards the growth of the strawberry plant, the effect of the cow urine fermented on the growth of the strawberry plant, and the effect of the liquid organic fertilizer from the cattle dung and cow urine fermented towards the growth of the strawberry plant. The research was designed by using complete randomized design with three replications with 36 treatments of the types and concentrations of the liquid organic fertilizer and cow urine. The result of the research indicates that the cow liquid organic fertilizer gives the best results on the plant height and the number of leaves of the strawberry plant, whereas the rabbit liquid organic fertilizer gives the best results on the number of flower on every strawberry plant. The addition of the cow urine (50 mL L-1) gives the best result on the growth of the strawberry plant. The cow liquid organic fertilizer (6 mL L-1) and the cow urine (50 mL L-1) give the best results on the plant height and number of leaves, whereas the rabbit liquid organic fertilizer (6 mL L-1) and the cow urine (50 mL L-1) give the best results on the number of flower of every strawberry plant.

Keywords: cattle dun, cow urine, growth of strawbery, liquid organic biofertilizer
Karakteristik Hidrolisat Protein Tambelo (Bactronophorus sp.) yang Dihidrolisis Menggunakan Enzim Papain

Karakteristik Hidrolisat Protein Tambelo (Bactronophorus sp.) yang Dihidrolisis Menggunakan Enzim Papain

Authors: Lely Okmawati, Rosmawati Rosmawati
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Abstract

Tambelo is one type of wood eating worm that live in the dead mangrove tree and suffered a decomposition process. Tambelo who lived in a wooden rod Rhizophora sp has a sweet taste and very good for consumption. Tambelo nutrient content showing potentially in the development of high-quality aquatic products, but this has not been done. In order tambelo can be used widely, it is necessary to make a new innovation in processing Tambelo meat into new face. Tambelo meat processing in a protein hydrolyzate as a source of flavor enhancer, which not only serves to create a sense of savory (umami) in food but also provides the role of nutrition and safe for the body considered very precise. This study aimed to examine the potential characteristics tambelo protein hydrolyzate (Bactronophorus thoracites). The experiment was conducted in two stages, the first stage of making a protein hydrolyzate treated hydrolyzate time (1, 2 and 3 hours) using the papain enzyme 0.5% (w/w) and temperature of 550C, pH 7. The best results are based on the content of the highest total protein become a reference in the second stage of the process. The second stage is a producing flavor enhancer, which further analyzed to measure the percentage of yield, amino acids, fatty acids and components of flavor. Proximate analysis to measure the highest total protein content of tambelo meat which hydrolyzed by the papain enzyme with optimum concentration at temperature of 55 ° C, pH 7. One hour hydrolysis showed the highest total protein content of 22.09% ± 0.19, yield hydrolyzate produced from the best treatment is 53.29% ± 7.36, hydrolysis process runs perfect for being able to produce 17 kinds of amino acids consisting of essential amino acids and non-essential with increased levels, and the hydrolysis process causes a significant fatty acid composition decreasing.

Keywords: hydrolysis, papain enzyme, Tambelo protein
Karakterisasi Bakteri Genus Vibrio Dari Ikan Kerapu (Plectropomus sp.)

Karakterisasi Bakteri Genus Vibrio Dari Ikan Kerapu (Plectropomus sp.)

Authors: Ar Syarif Hidayat
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Abstract

This research is about the isolation and characterization Vibrio sp. from grouper fish (Plectropomus sp.). This research aimed to observe the amount of vibrio bacteria species based on colony morphology and characterization morphological colony of vibrio isolated from grouper fish (Plectropomus sp.). Bacteria isolation was conducted by isolate some external organs (lesion on its body) and internal organ (jejenum) from grouper fish (Plectropomus sp.). Bacteria was grown on selective medium for vibrio, TCBS (Thiosulfate Citrate Bile Sucrose) agar. The incubation time was 24 hours at 370C. The result showed there are 3 isolate vibrio bacteria that was coded by V1, V2, and V3. V1 morphological characteristis was rounded, edge is entire, convex elevation, green colony colour with blue in the center of colony and smooth tekstur. V2 morphological characteristic was rounded, edge is entire, tortous elevation, colony colour yellow, and smooth tekstur. Morphological characteristic of V3 was Irregular, egde is Undulate, elevation hill, colony colour yellow and own the smooth tekstur.

Keywords:
Uji Aktivitas Antibakteri Ekstrak Kloroform dan Ekstrak Etanol Biji Bidara Laut (Strychnos ligustrina Bl) Terhadap Staphylococcus aureus ATCC 25923 dan Salmonella thypi

Uji Aktivitas Antibakteri Ekstrak Kloroform dan Ekstrak Etanol Biji Bidara Laut (Strychnos ligustrina Bl) Terhadap Staphylococcus aureus ATCC 25923 dan Salmonella thypi

Authors: Eti Sumiati
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Abstract

A research has been conducted on the antibacterial activity of Strychnos ligustrina seeds against Staphylococcus aureus ATCC 25923 in vitro. Strychnos ligustrina seeds extracted using two solvents, namely chloroform (chloroform extract) and 70% ethanol (ethanol extract) and analysis of the chemical content of the extract by thin layer chromatography and paper chromatography. The results of this study are expected to provide data activity of Strychnos ligustrina seeds as antibacterial scientifically. In this study, the chloroform extract and ethanol extract of Strychnos ligustrina seeds obtained by the filtration method with Soxhlet apparatus. Antibacterial activity test of chloroform extract and ethanol extract 70% from Strychnos ligustrina seeds performed using liquid dilution method, the parameters used are the MIC (minimum inhibitory concentration) and MKC (minimum killing concentration). MIC is determined by turbidity or clarity of the test solution, while MKC determined by scraping the respective concentrations of the extract / test solution on blood agar medium. The results showed that the chloroform extract and ethanol extract of Bidara Laut seed has antibacterial activity against Staphylococcus aureus ATCC 25923 and Salmonella thypi. MIC of each test extracts (chloroform and ethanol 70%) could not be determined because both of the test extract produce turbid color, while the MIC of the second test extract was 3.62% b/v for Salmonella thypi, 8.75% for Staphylococcus aureus ATCC 25923 (Strychnos ligustrina seeds chloroform extract), 22.5% for Salmonella thypi and 15% for Staphylococcus aureus ATCC 25923 (Strychnos ligustrina seeds ethanol extract). Results of thin layer chromatography and paper chromatography showed that the chloroform extract seeds contain alkaloids and steroids / triterpenoids, whereas the 70% ethanol extract contains alkaloids, tannins and steroids/triterpenoids.

Keywords: antibacterial, chloroform extract, ethanol 70% extract, Strychnos ligustrina seeds, Salmonella thypi, Staphylococcus aureus
Efek Hepatotoksisitas Ekstrak Etanol Herba Kompri (Symphytum officinale L.) Terhadap Hewan Uji Kelinci (Oryctolagus cuniculus) Dengan Parameter SGOT dan SGPT

Efek Hepatotoksisitas Ekstrak Etanol Herba Kompri (Symphytum officinale L.) Terhadap Hewan Uji Kelinci (Oryctolagus cuniculus) Dengan Parameter SGOT dan SGPT

Authors: Norma Norma, Ulfa Triyani, Syamsidar Usman
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Abstract

This study aims to determine the hepatotoxic effects of extract ethanol Herba Comfrey the rabbit test animals by measuring levels of SGOT and SGPT. This study used 12 rabbits were divided into 4 groups. The control group (-) given Na.CMC 1% w/v, group I, II, and III were given suspensions of extract ethanol Herba Comfrey with each concentration of 2% w/v, 4% w/v and 8% w/v for 7 days. The results showed that the extract ethanol Herba Comfrey with concentration of 2% w/v, 4% w/v, and 8% w/v effects can cause hepatotoxicity. It is showed from the increase in the average SGOT value in rabbits at 13.96 U/L, 20.63 U/L and 37.13 U/L. and SGPT value of 20.73 U / L, 32.4 U / L and 44.66 U / L. extract Ethanol Herba Comfrey with concentration 8% w / v causes an increase in SGOT and SGPT concentrations higher than the other, and accelerate the liver damage or hepatotoxicity. The higher the concentration, the greater the effect.

Keywords: Herba Comfrey, hepatotoxicity, SGOT-SGPT
Pengaruh Pemberian Variasi Dosis Pupuk Kandang Terhadap Pertumbuhan Tanaman Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.)

Pengaruh Pemberian Variasi Dosis Pupuk Kandang Terhadap Pertumbuhan Tanaman Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.)

Authors: Dewi Nur Anggraeni
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Abstract

Research on the effect of dose variations of manure on God’s Crown (Phaleria macrocarpa (Scheff.) Boerl.) growth was implemented in Ambarketawang, Gamping, Sleman, Yogyakarta. This study aimed to determine the effect of administration of dose variations of manure on God’s Crown growth. This research method using a complete randomized design (CRD) with one treatment factors which is administrating manure into polybags containing 2 kg of soil with dose variations of 0 g, 50 g, 100 g, 150 g, 200 g, and 250 g. The parameters measured in this study are height, leaf number, and fresh weight of Crown’s God plant. The results were analyzed by analysis of variance (ANOVA), if the data is meaningful (significant difference) then followed by LSD test level of 5%. The results obtained are the effects of administration of manure dose variation produce different Crown’s God (Phaleria macrocarpa (Scheff.) Boerl.) growth, and the best dose for Crown’s God (Phaleria macrocarpa (Scheff.) Boerl .) growth is 250 grams.

Keywords: crown’s god (Phaleria macrocarpa (Scheff.) Boerl.), dose of manure

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