Egyptian Journal of Agronematology

Egyptian Journal of Agronematology

Basic info

  • Publisher: Egyptian Society for Agronematology
  • Country of publisher: egypt
  • Date added to EuroPub: 2019/Nov/09

Subject and more

  • LCC Subject Category:
  • Publisher's keywords:
  • Language of fulltext: english

Publication charges

  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? No

Editorial information

Open access & licensing

  • Type of License:
  • License terms
  • Open Access Statement: No
  • Year open access content began: 1998
  • Does the author retain unrestricted copyright? False
  • Does the author retain publishing rights? False

Best practice polices

  • Permanent article identifier:
  • Content digitally archived in:
  • Deposit policy registered in:

This journal has '48' articles

Nematicidal properties of some algal aqueous extracts
against root-knot nematode, Meloidogyne incognita in vitro

Nematicidal properties of some algal aqueous extracts against root-knot nematode, Meloidogyne incognita in vitro

Authors: Ahmed Nour El-Deen
( 0 downloads)
Abstract

The effectiveness of aqueous extracts derived from nine algal species at different concentrations on egg hatching and mortality of Meloidogyne incognita (Kofoid and White) Chitwood juveniles after various exposure times were determined in vitro. Results indicated that Enteromorpha flexuosa at the concentration of 80% was the best treatment for suppressing the egg hatching with value of 2 % after 5 days of exposure, followed by Dilsea carnosa extract (3%) and Codium fragile (4%) at the same concentration and exposure time. Likewise, application of C. fragile, D. carnosa , E. flexuosa and Cystoseira myrica extracts at the concentrations of 80 and 60% were highly toxic to the nematodes, killing more than 90 % of nematode larva after 72 hours of exposure while the others gave quite low mortalities. The characteristic appearances in shape of the nematodes killed by C. fragile, D. carnosa , C. myrica, E. flexuosa and Sargassum muticum was sigmoid (Σ-shape) with some curved shape; whereas, the nematodes killed by other algal species mostly followed straight or bent shapes. The present study proved that four species of algae C. fragile, D. carnosa, C. myrica and E. flexuosa could be used for the bio-control of root-knot nematodes. Keywords: Algae, In vitro, Meloidogyne incognita, Nematicidal activity

Keywords: Algae, In vitro, Meloidogyne incognita, Nematicidal activity
Potential Use of Botanicals Rich in Alkaloids for Controlling
Meloidogyne incognita and Rotylenchulus reniformis
Infecting Cucurbits

Potential Use of Botanicals Rich in Alkaloids for Controlling Meloidogyne incognita and Rotylenchulus reniformis Infecting Cucurbits

Authors: Fatma Mostafa, A. Refaei, Marwa El-Deriny
( 0 downloads)
Abstract

Two greenhouse experiments were conducted to evaluate the nematicidal activity of certain alkaloid plants namely, periwinkle (Catharanthus roseus), datura (Datura stramonium), lupin (Lupinus albus), pomegranate (Punica granatum) and fenugreek (Trigonella foenum-graecum) against root-knot nematode Meloidogyne incognita infecting cucumber (Cucumis sativus) and reniform nematode (Rotylenchulus reniformis) infecting cantaloupe (Cucumis melo var.cantaloupensis). Dried powdered parts of such plants were screened at three rates (1, 3 and 5g / plant). In most treatments data indicated a positive correlation between tested rates and plant biomass. Dried leaf powder of C. roseus (62.5%) as well as dried seed powder of L. albus ( 52.8%), and T. foenum-graecum ( 43.2 %) induced remarkable improvement in total plant fresh weight of cucumber at the rate of 5g/plant. However, dried leaf powder of D. stramonium (41.4%) recorded a significant increase in total plant fresh weight at the rate of 3g/plant. By all treatments of alkaloid plants root-knot nematode population whether in soil and roots, root galling and number of egg masses were significantly suppressed. Dried leaf powder of C. roseus at three tested rates (71.8, 71.6 and 72.6%) performed the best and suppressed total nematode population of M. incognita followed by T. foenumgraceum at 5g/plant (70.1%). On the other hand, a slight to moderate improvement in plant growth parameters of cantaloupe infected with R. reniformis was indicated in pots receiving alkaloids plants. The highest percentage of increase in total plant fresh weight was noticed with dried leaf powder of C. roseus at three tested rates (28.5, 31.4 and 32.6%) respectively. The best protection of cantaloupe against R. reniformis was recorded with dried seed powder of T. foenum-graecum (57.9, 65.2 and 75.0%) and P. granatum (55.4, 55.9 and 61.7%) at three tested rates, respectively which ultimately suppressed R. reniformis population.

Keywords: Meloidogyne incognita, Rotylenchulus reniformis, cucumber, cantaloupe, alkaloid plants.
Efficacy of some Bioagents, Algal Species and Plant
Extracts in Controlling Meloidogyne Incognita on Yield of
Grape Vines in Egypt

Efficacy of some Bioagents, Algal Species and Plant Extracts in Controlling Meloidogyne Incognita on Yield of Grape Vines in Egypt

Authors: Samaa Shawky, Ahlam Al-Ghonaimy, Eman Hammad
( 0 downloads)
Abstract

These experiments were conducted to control the root-knot nematodes, Meloidogyne incognita under both greenhouse and field conditions in superior seedless vineyards and its reflection on yield. The field experiment was carried out in a private vineyard (El Wady El Faregh) in Alexandria-Cairo road (kilo 78). Seven treatments were used as follows: two bioagents (Bacillus subtilis and Trichoderma harzianum), two blue green strains algal species (Cyanobacteria) (Spirulina platensis and Oscillatoria sp.) and two plant aqueous extracts (Ambrosia maritime and Eucalyptus globules) at three concentrations, and in comparison with oxamyl (24% EC) were used to control Meloidogyne incognita on superior seedless vineyards on grapes under both greenhouse and field conditions. Bioagents, algal species and plant extracts were added to the soil under vines one time weekly for three weeks after bud burst under both greenhouse and field conditions. The most effective treatments in controlling root-knot nematodes; Meloidogyne incognita were both Spirulina platensis and Oscillatoria sp whereas the least effective was suspension of Eucalyptus globules under both greenhouse and field conditions. Both Spirulina platensis and Oscillatoria sp acheived the highest reduction effect on number of 2nd stage juveniles in soil, numbers of galls, developmental stages, females, egg masses, egg numbers/egg mass in roots and final population Meloidogyne incognita on grape plants, whereas the suspension of Eucalyptus globules was the least effective treatment under both greenhouse and field conditions. In addition all treatments increased fresh weight of the whole plant over control under greenhouse conditions. Results showed that all the treatments had a significant effect in reducing the total population and build up of root – knot nematodes, Meloidogyne incognita in both soil and roots especially after three months from the time of application. In addition, all treatments had increased the yield of superior seedless grapevines under field conditions.

Keywords: Meloidogyne incognita, control, superior seedless grapevines, bioagents, algal species and plant aqueous extracts.
Electrophoretic Patterns of Some Populations of
Meloidogyne Arenaria and M. javanica

Electrophoretic Patterns of Some Populations of Meloidogyne Arenaria and M. javanica

Authors: Mahfouz Abd-Elgawad, Mohamed Eissa, Abd-Elmoneim El-Gindi, Ahmed Osman, Grover Smart
( 0 downloads)
Abstract

Root-knot nematodes (Meloidogyne spp.) are important polyphagous plant parasites worldwide. Intraspecific variation and the occurrence of physiological races have necessitated the development of taxonomic techniques enabling differentiation and classification of races related to major Meloidogyne spp. Accurate identification of their races and populations is essential for determining appropriate management approaches and benefits from further characterization and understanding of the variability within a single nematode species. Electrophoretic focusing of malic dehydrogenase, α-glycerophosphate dehydrogenase and esterase for seven populations of Meloidogyne spp. by polyacrylamide gels was tested. These populations included 1, 2 and 4 populations of M. javanica and M. arenaria race I and II, respectively. Malic Dehydrogenase profiles obtained by polyacrylamide-gel electrophoresis indicated that differences between Meloidogyne arenaria and M. javanica were more pronounced than were differences between races or populations of M. arenaria. The enzyme patterns of the two species varied even though the nematodes were propagated on the same host plant (Rutgers tomato).

Keywords: Biochemical markers, Meloidogyne arenaria, M. javanica, root-knot nematode, molecular taxonomy.
Long-term effect of Tylenchulus semipenetrans on citrus
tree quality in reclaimed land of Egypt

Long-term effect of Tylenchulus semipenetrans on citrus tree quality in reclaimed land of Egypt

Authors: M. Abd-Elgawad, F. Koura,, S. Montasser, Ahmed El-bahrawy
( 0 downloads)
Abstract

Citrus fruit yield is considered a short-term response to the citrus nematode, Tylenchulus semipenetrans damage and therefore this study addressed parameters measuring overall appearance of the sampled trees. Soil and roots from 20 trees in each of three mature: 'banzaheer' lemon, 'Baladi' mandarin, and ‘Navel’ orange and one immature: ‘Navel’ orange orchards, all grafted on sour orange rootstock, in Egypt were sampled for the citrus nematode, T. semipenetrans, in February 2011 to investigate their correlations with tree parameters (tree height and vigor, canopy diameter, and trunk circumference) that reflect long-term nematode damage of the sampled trees. The correlation between pairs of these four parameters were always positive and generally with high significant levels. Each of the four parameters was not significantly correlated with fruit yield of the mature trees but each of tree height and vigor, and canopy diameter was correlated with fruit yield of the immature Navel orange. The relationships between pairs of these biotic parameters and T. semipenetrans population density in fibrous roots and soil were not consistent. Eleven edaphic factors in mandarin and mature orange orchards were measured and compared. Both soils were dominated by rough grainy particles of sand. Levels of potassium, calcium, phosphorus, ammonium and nitrate as well as soil solution pH, organic matter content and electric conductivity in the soil samples were significantly (P ≤ 0.01) different between the mandarin and orange orchards. To achieve better economic revenue of citriculture, local tackling of T. semipenetrans problem in newly reclaimed areas of Egypt was discussed.

Keywords: citrus, crop-loss assessment, Tylenchulus semipenetrans management, Egypt.
Influence of Meloidogyne javanica and Rotylenchulus
reniformis alone on nodulation of some leguminous crops

Influence of Meloidogyne javanica and Rotylenchulus reniformis alone on nodulation of some leguminous crops

Authors: S. Montasser, N. Mahmoud, A. El-Mesalamy, M. Abdel-Mageed
( 0 downloads)
Abstract

The effect of root-knot nematode, Meloidogyne javanica and the reniform nematode, Rotylenchulus reniformis alone on nodulation of four Rhizobium species on roots of 27 cultivars related to six leguminous species were evaluated. The formation of nodules on their roots varied greatly in their numbers according to host species, host cultivars, rhizobal species and nematode species. The greater nodulation reduction in infected roots with nematode species, M. javanica and R. reniformis was observed on roots of common bean cvs. Exira, Nebraska, Polista and Savana inoculated with rhizobial species Rhizobium phaseoli and lupine cv. Giza 2 inoculated with rhizobial species R. lupine. In general, inoculation of M. javanica or R. reniformis with rhizobial species greatly reduced root nodulation as compared to leguminous species inoculated with Rhizobium species alone. Also, it is important to note that the reduction in the number of bacterial nodules was more pronounced with R. reniformis infection than with M. javanica infection. It is noteworthy that the number of nodules on roots of broad bean cv. Wade 1 inoculated with R. phaseoli increased by both nematode species infections.

Keywords: Rhizobium, nodulation, Meloidogyne javanica, Rotylenchulus reniformis, leguminous.
Influence of Five plant dried leaf powders on eggplant plants infected with Meloidogyne incognita under greenhouse conditions

Influence of Five plant dried leaf powders on eggplant plants infected with Meloidogyne incognita under greenhouse conditions

Authors: S. Gad, M. Sergany
( 0 downloads)
Abstract

A greenhouse trail was conducted to study the influence of five plant dried leaf powders i.e. cabbage, sorrel, spinach, rocket and tomato in comparing with oxamyl on root-knot nematode M. incognita infecting eggplant under greenhouse conditions. All treatments showed remarkable increase in plant growth parameters as well as reduced nematode criteria. Cabbage dried leaf powder showed higher effectiveness of the tested botanical dried leaf powders and enhanced plant growth parameters with significant increase values. On the other hand, spinach dried leaf powder as soil amendments overwhelmed other treatments in suppressing nematode population in soil (78.3%), root galling number (87.0%), egg-masses (91.5%) and eggs / one gram root (91.5%) respectively. However, the least values of the same nematode criteria was achieved by sorrel treatment with values of 34.0,77.3,90.5 and 89.3 respectively, comparing to nematode alone. The recorded results showed a potential properties of using such plant dried leaf powders as eco-friendly control method instead of chemical pesticides

Keywords: Eggplant, Meloidogyne incognita, control, oxamyl, induced resistance, plant, powder.
Integrated Management of Root-Knot Nematode, Meloidogyne javanica Infected Cucumber and Courgette

Integrated Management of Root-Knot Nematode, Meloidogyne javanica Infected Cucumber and Courgette

Authors: M. Abo-Korah
( 0 downloads)
Abstract

This work was conducted to study the effect of nematode – trapping fungus (Arthrobotrys conoides); white wormwood (Artemisia herba alba) and the nematicides, Tervigo (half dose) in the integrated control programs of plant parasitic nematodes. These agents were evaluated alone as well as in combination against Meloidogyne javanica infected Cucumber and Courgette under greenhouse conditions, at the Experimental Farm, Faculty of Agriculture, Menoufia University, Shibin El-Kom, Egypt. The obtained results showed that, the application of A. conoides + Wormwood + Tervigo treatment gave the highest reduction percentage of the nematode with reduction ratio of (85.0%) followed by the treatment of A. conoides + Tervigo (77.3%) in Cucumber plants and the same previous treatments gave (80.0% and 70.5%) in Courgette plants, respectively. Moreover, the two previous applications gave better enhancement in various growth parameters such as, plant height, shoot weight, and root weight in both Cucumber and Courgette plants. The same application gave the lowest gall index (1.0) representing the highest total decrease in gall numbers (-83.3%) in cucumber and (-81.8%) in Courgette compared with control. There are significant differences in root gall index between control treatment and all other treatments. Results indicate that Tervigo as a nematicide had no adverse effects on the predaceous activity of A. conoides. Moreover, dried leaves of Wormwood play an important role in nematode control, as well as it promoting plant growth and enhancing beneficial microorganisms in soil.

Keywords: IPM, Meloidogyne, Wormwood, Arthrobotrys conoides, biological control
Effects of Plant Leaf Extracts on Egg-Masses Hatching and Juveniles Mortality of the Root-Knot Nematode, Meloidogyne Javanica

Effects of Plant Leaf Extracts on Egg-Masses Hatching and Juveniles Mortality of the Root-Knot Nematode, Meloidogyne Javanica

Authors: A. Aanany, N. Mahmoud
( 0 downloads)
Abstract

The effects of plant extracts from fresh leaves of five different plants; Acasia arabica, Ziziphusspina christi, Eucalyptus camaldulensis, Lawsonia inermis and Dalbergia sissoo were evaluated against egg-masses hatching and juvenile mortality of root-knot nematode, Meloidogyne javanica under laboratory conditions. Results revealed that, all treatments extracts had significantly (P < 0.05) reduced in the hatching of egg-masses. The maximum reduction occurred in Acasia arabica with the concentration (S) was (97.13%) and the concentration (S/2) was (89.99%), whereas the minimum reduction caused by Ziziphusspina christi with the concentration (S) was (45.49%) and the concentration (S/2) was (24.63%). The same effects of plant extracts against M. javanica juveniles, the most mortality of second stage juveniles was observed in leaf extracts of Acasia arabica, and Dalbergiasissoo with the concentration (S) was (100%) as regarding to and the concentration (S/2) was (100%) in Dalbergia sissoo, while the least in Ziziphusspina christi with the both concentrations S and S/2 (17.0 and 5.33%), respectively.

Keywords: Root-knot nematode, Meloidogyne javanica, plant leaf extracts.
Host Suitability of Four Potato Cultivars To Meloidogyne Incognita Infection Under Greenhouse Conditions

Host Suitability of Four Potato Cultivars To Meloidogyne Incognita Infection Under Greenhouse Conditions

Authors: A. El-Sherif, S. Gad, Mayada Hefny
( 0 downloads)
Abstract

Greenhouse experiment was conducted to evaluate suitability of four potato cultivars i.e. Cara, Draga, Spunta and Solana against M. incognita infection at 20±3°C. Results indicated that none of the tested potato cultivars was immune to nematode infection since galls or egg masses on root system of such cultivar was recorded and all plant growth parameters were obviously diminished. Among the tested potato cultivars, Spunta showed the highest percentage reduction values of all plant growth characters. Host category of the tested cultivars was determined according to the relationship between host growth response in term of reduction % of whole plant fresh weight and R Factor recorded that Cara and Draga potato cultivars were classified as moderately resistant (MR), whilst potato cvs. Spunta and Solana were rated as highly susceptible (HS) and susceptible (S), respectively.

Keywords: Potato cultivars, host suitability Meloidogyne incognita, greenhouse
Plant-Parasitic Nematodes Associated with Forage Legumes Planted in Fallow and into Living Grass Sod

Plant-Parasitic Nematodes Associated with Forage Legumes Planted in Fallow and into Living Grass Sod

Authors: Mahfouz Abd-Elgawad, Mohamed Eissa, Abd-Elmoneim El-Gindi, Grover Smart
( 0 downloads)
Abstract

The capability of legumes to fix and provide sufficient nitrogen for crop production has encouraged re-appraisal of cropping systems that utilize relevant forage legumes. However, plant-parasitic nematodes (PPN) can damage forage legumes but differently according to forage legume production systems. Therefore, this study determined the population levels of PPN associated with three different production systems. Different species of forage legumes were planted in fallow soil and into living grass sod of two species; 'Pensacola' bahia grass and 'Tif-81' bermuda grass. Thirteen genera of PPN were associated with one or more cultivars of twelve forage legumes in one or more of three locations in Alachua County near Gainesville, Florida, USA. No significant difference (P ≤ 0.05) was found in the nematode population levels among clover cultivars overseeded in dormant bahia grass or bermuda grass sod. However, when clover cultivars were planted in fallow plots, significant differences (P ≤ 0.05) occurred in population levels of Meloidogyne spp. Also, significant differences (P ≤ 0.05) occurred in populations of Meloidogyne spp. and Criconemella spp. among the three experiments.

Keywords: bahia grass, bermuda grass, forage legume production systems, plant-parasitic nematodes
Development of Plant-Parasitic Nematode Populations on Forage Crops under Field Conditions

Development of Plant-Parasitic Nematode Populations on Forage Crops under Field Conditions

Authors: Mahfouz Abd-Elgawad, Mohamed Eissa, Abd-Elmoneim El-Gindi, Grover Smart
( 0 downloads)
Abstract

The continuous increase in cost and scarcity of mineral fertilizers resulting from the use of high-cost energy sources has renewed interests in organic recycling and biological nitrogen-fixation to improve soil fertility and productivity. Forage legumes can fix nitrogen from atmosphere which has the merit to offer nitrogen for both crop production and soil fertility. However, plant-parasitic nematodes (PPNs) interactions with leguminous crops can be agronomically damaging under various conditions. The present study examined population development of each of the PPN genera Meloidogyne spp., Paratrichodorus spp., Criconemella spp., Pratylenchus spp., Tylenchorhynchus, and Tylenchus spp. on fourteen legume cultivars (i.e. 'Florida' red clover, 'Nolin' red clover, 'Kenland' red clover, 'Kenstar' red clover, 'Tibbee' crimson clover, 'Dixie' crimson clover, 'Mt. Barker' Subterranean clover, 'Amclo' arrowleaf clover, 'FL - 77' alfalfa, 'Abon' persian clover, 'Wood ford' big flower vetch, 'Seqrest' ball clover, 'Kondinin' rose clover, and 'Chief' crimson clover). The nematode initial and final populations varied considerably among the legume cultivars. Therefore, we adjusted for the rate of nematode multiplication by using analysis of covariance. However, we could not use one of the statistical multiple comparison methods because each treatment had different mean of squares for the experimental error. Consequently, a matrix was designed to test the significance of nematode populations on the cultivars using the t-test. Using a computer program, the exact (numerical) level of statistical significance for nematode densities among the cultivars could be established. Relatively lower populations of the nematode genera were found on 'FL-77' alfalfa plants.

Keywords: analysis of covariance, forage legumes, nematode population development
Phytochemical Screening and Nematicidal Activity of  Cinnamon and Ginger Extracts Against Root-knot Nematode (Meloidogyne incognita) Infecting Tomato

Phytochemical Screening and Nematicidal Activity of Cinnamon and Ginger Extracts Against Root-knot Nematode (Meloidogyne incognita) Infecting Tomato

Authors: Dina Ibrahim, Rania Hussein
( 0 downloads)
Abstract

The purpose of this work was to assess the nematicidal and antioxidant activities of different extracting solvents from cinnamon (Cinnamomum zeylanicum) and ginger (Zingiber officinale) against Meloidogyne incognita infecting tomato. Three solvents namely n-hexane, ethyl acetate and methanol were used. Phytochemical analysis of such extracts revealed the presence of more components in ethyl acetate and methanolic extracts than n-hexane extract of either cinnamon or ginger. The highest amount of total phenolics and flavonoids were detected in ethyl acetate extract of both ginger and cinnamon reaching 105.6; 93.6 mg gallic acid /g and 54.2; 45.1 mg quercetin/ g dry weight, respectively. Additionally, ethyl acetate and methanolic extracts of ginger exhibited the highest antioxidant activity (DPPH free radicals) reaching 90.0 and 89.1%, respectively. The efficacy of cinnamon and ginger using three solvents at two concentrations (50 and 10ul) on juveniles mortality of Meloidogyne incognita was studied in vitro. Ethyl acetate extracts gave better results than did methanol or hexane extracts. Ginger extracts gave promising results after 72hr of exposure compared to cinnamon ones. Hence, among ginger extracts the maximum mortality in nematode juveniles was achieved with ethyl acetate (68.0 %) followed by methanol (42.0%) and hexane extracts (30%) @ the concentration of 50µl after 72 hours of exposure. Ginger ethyl acetate (300 ppm) proved to be the best for enhancing total plant fresh weight with percentage of increase over control amounted to 196.0 %. Population densities, root galling and number of egg masses were significantly suppressed with such tested extracts. Leaves of tomato were assayed for NPK, total cholorophyll, proteins, and phenols. Activities of polyphenol oxidase (PPO), peroxide oxidase (PO) were also evaluated in roots of tomato infected with M. incognita. PO activity was much greater in ginger ethyl acetate at lower concentration (100 ppm). Conversely, PPO activity was increased in cinnamon ethyl acetate (100 ppm).The present study revealed high potential antioxidants and nematicidal properties in both cinnamon and ginger. The biological activities of these plants might be attributed to the various kind of secondary metabolites. Key words: Cinnamomum zeylanicum, Zingiber officinale, nematicidal activity, antioxidant, total phenolics, flavonoids, polyphenol oxidase, peroxide oxidase, Meloidogyne incognita.

Keywords: Cinnamomum zeylanicum, Zingiber officinale, nematicidal activity, antioxidant, total phenolics, flavonoids, polyphenol oxidase, peroxide oxidase, Meloidogyne incognita.
Phytoparasitic Nematodes  Associated with Different  Cultivars of Grape Grown in Two Type of Soil in Egypt.

Phytoparasitic Nematodes Associated with Different Cultivars of Grape Grown in Two Type of Soil in Egypt.

Authors: M. Mohamed, A. Korayem, S. Montasser, Saeid Ananay, D. Al-Baghdady
( 0 downloads)
Abstract

Survey was conducted in Giza, qualiabia and Behaira governorates of Egypt during 2010-2011 seasons, to study the occurrence and population density phytoparasitic nematodes associated with grapes (Vitis). A total number of 160 soil and root samples were collected from the rhizospher of six grape cultivars Bez-Alanza, Flame seedless, King Ruby, Romy Red, Superior and Thomoson seedless were collected by digging soils to a depth of 32-40 cm with stainless steel half –tubes. The results indicated that the present of ten phytonematode genera, Criconemoides, Ditylenchus, Helicotylenchus, Hoplolaimus, Meloidogyne, Pratylenchus, Rotylenchulus, Tylenchorhynchus, Tylenchulus and Xiphinema. Frequency and population density of each nematode genera different according to grape cultivars and soil type. The root knot nematode (Meloidogyne) was prevalent nematode in all cultivars and localities it was present in 77.32% of total samples. Also root knot nematode (Meloidogyne) was frequent in (F.O=72.5%) than in clay soil (16.77%),while the citrus nematode (Tylenchulus) was more frequent in clay soil (66.71) than in sandy soil (0.67%).

Keywords: Plant parasitic nematodes, frequency, grapevine, Egypt.
Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles

Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles

Authors: S. Kassab, M. Eissa, U. Badr, A. Ismail, Gaziea Soliman
( 0 downloads)
Abstract

Serratia marcescens (SM) is currently considered as a biocontrol agent against plant parasitic nematodes. It is one of the most effective bacteria for degradation of chitin. This lytic bacteria was evaluated on the survival of Meloidogyne incognita juveniles under laboratory conditions. The best treatment by (SM 36) mutant achieved zero viable juveniles in either S or S/10 i.e, highly effective on juveniles mortality compared to the wild type of SM and untreated control which recorded 51.8, 49.3 and 49 juveniles, respectively. The numbers of non viable juveniles of the best treatment were 6, 4 and 2.8 individuals after 24, 48 and 72 hrs, respectively compared to the SM wild type which achieved 26.3. 33.3 and 27.3 while the untreated control did not show any effect on the juveniles. There were positive relationships between the nematode mortality and each of the bacteria concentration and enzyme production from the mutants. The numbers of either viable juveniles or non viable juveniles were reduced at S or S/10 dilutions and exposure periods of 24, 48 and 72 hr compared to the untreated control. This reduction was attributed to the dead nematode bodies which were degraded and destroyed by these bacterial mutants. Mutation achieved increasing of chitinase and alkaline protease-over producing mutants, which produced two to three times more endochitinase activity than the wild type of S.marcescens.

Keywords: lytic bacteria, chitinase, protease, Serratia marcescens, mutation Meloidogyne incognita.

About Europub

EuroPub is a comprehensive, multipurpose database covering scholarly literature, with indexed records from active, authoritative journals, and indexes articles from journals all over the world. The result is an exhaustive database that assists research in every field. Easy access to a vast database at one place, reduces searching and data reviewing time considerably and helps authors in preparing new articles to a great extent. EuroPub aims at increasing the visibility of open access scholarly journals, thereby promoting their increased usage and impact.