Journal of Veterinary Medical Research

Journal of Veterinary Medical Research

Basic info

  • Publisher: Beni-Suef University
  • Country of publisher: egypt
  • Date added to EuroPub: 2018/Aug/20

Subject and more

  • LCC Subject Category: Veterinary Science
  • Publisher's keywords: Veterinary Medicine, Pathology, Anatomy & Embryology, Physiology, Clinical Pathology, Parasitology, Biochemistry, Pharmacology and Microbiology, Immunology
  • Language of fulltext: english

Publication charges

  • Article Processing Charges (APCs): Yes 100USD
  • Submission charges: No
  • Waiver policy for charges? No

Editorial information

Open access & licensing

  • Type of License: CC BY
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 1990
  • Does the author retain unrestricted copyright? True
  • Does the author retain publishing rights? True

Best practice polices

  • Permanent article identifier: None
  • Content digitally archived in: Nopolicy
  • Deposit policy registered in: None

This journal has '103' articles

Fungal pathogens associated with respiratory problems in broiler chickens

Fungal pathogens associated with respiratory problems in broiler chickens

Authors: Radwan I. A., Abed A. H.*, Abd El-Aziz M. M. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef Universit...
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Abstract

Fungal diseases of poultry have become problematic as bacterial and viral diseases. Therefore, this study was designed to investigate the prevalence of fungal agents in broiler chickens suffering from respiratory disorders. The prevalence rate of fungal isolation was 53.1% including mycelia fungi (42%) and yeast isolates (11.1%). Mould isolates were identified as Aspergillus fumigatus (21.7%), Aspergillus flavus (8.4%), Aspergillus niger (8.4%), Aspergillus nidulans (1.3%), Cladosporium spp. (0.4%) and Penicillium spp. (1.8%). Concerning yeast isolates, Candida spp. was the most predominant which were identified as C. albicans, C. pseudotropicalis (2.7% each), C. krusei, C. regosa (2.2% each), and C. stellatoidea (0.4%). Moreover, one Cryptococcus sp. was recovered and identified as C. neoformans (0.9%). PCR assay using oligonucleotide primer amplifying a 570 bp fragment based on ITS region gene was conducted on randomly selected 9 isolates including 6 Aspergillus spp. (A. flavus, A. niger, A. fumigates; 2 each) as well as 3 yeast isolates (two isolates of C. albicans and one isolate of C. neoformans). All tested isolates had positive reactions.

Keywords: Fungi, broilers, respiratory affection, Aspergillus, Cladosporium
Rumen impaction in cattle due to plastic materials

Rumen impaction in cattle due to plastic materials

Authors: Akraiem A.a, Abd Al-Galil A. S. A. b,* a Department of Veterinary Medicine and Surgery, Faculty of Veterinary Medicine, Omar Al-Mukhtar University,...
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Abstract

The present study was carried out on 22 cows suffering from ruminal impaction with plastic materials as foreign bodies and ten apparently healthy cows as a control group. Clinical examination included clinical signs, temperature, pulse, respiratory rate and ruminal motility were recorded prior to treatment. Hematological parameters such as hemoglobin (Hb), packed cell volume (PCV), erythrocyte count (RBCs), total leucocytes (WBCs) count and the glutaraldehyde test were performed. Ruminal fluid was evaluated for pH and the methylene blue reduction time (MBRT). The mean pH of rumen fluid, MBRT, total leucocytes count, and PCV were increased significantly (P<0.05). Rumen motility was significantly reduced (P<0.05) preoperative in the animals suffering from rumen impaction, but the mean value of pulse rate, respiration rate, temperature, glutaraldehyde test, haemoglobin and total erythrocyte count were non-significantly changed. On the 5th postoperative day the clinical and the laboratory parameters in the study group had largely become normalized. Six months after the procedure, 18 (81.9%) cows showed complete recovery and 4 (18.9%) animals were slaughtered within 3 months following surgery. This study concluded that the clinical and laboratory findings might be of diagnostic importance. Rumen impaction with plastic materials should be differentiated from anorexia, emaciation, ruminal hypomotility, tympany and dehydration in cows. The surgical removal of foreign body demonstrated positive effects on animal health.

Keywords: Cattle, ruminal impaction, surgical elimiantion
Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus

Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus

Authors: Hassan W. H.a,*, Hatem M. E.b, Elnwary H. A.c, Sediek S. H.c a Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, B...
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Abstract

In the current study, a total of 20 and 78 milk samples were collected from animals showed signs of clinical and subclinical mastitis, for isolation and identification of different causative pathogens in some dairy farms of Beni-Suef Governorate, and for investigation of in vitro sensitivity. The recovered microorganisms were Staphylococcus species (n=79; 80.61%), Enterococcus spp. (n=28; 28.57%), CAMP negative Streptococci, Pseudomonas aeruginosa (n=7; 7.14%), E. coli (n=3; 3.06%) and Proteus vulgaris (n=1; 1.02%). Antibiogram profile for S. aureus showed that the most effective drug was vancomycin and the least was penicillin. Trials were done to detect biofilm production for recovered isolates of S. aureus (n=23) by the use of a phenotypic method (Congo red agar, CRA) and genotypic methods through determination of some biofilm related genes using PCR. All recovered S. aureus isolates were seeded on the CRA media to detect the biofilm forming ability. It has been found that all tested isolates showed a biofilm forming ability either strong (13; 56.52%) or intermediate (10; 43.48%). The detection of some biofilm associated genes (icaA, icaD and bap genes) using polymerase chain reaction revealed that two (10.53%) isolates out of 19 were negative for all tested genes, 16 (84.21%) isolates harbored both icaA and icaD gene, while only one (5.26%) isolate had all tested genes.

Keywords: Mastitis, antimicrobial sensitivity, S. aureus, biofilm
Decontamination of broiler carcasses` skin using medicinal herbal extracts

Decontamination of broiler carcasses` skin using medicinal herbal extracts

Authors: Fathy A. Khalafalla a, Fatma H.M. Ali a, Jehan M. Ouf b, Ahmed M.M.A. Mosa c,* a Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Sue...
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Abstract

This study was carried out to detect the efficiency of different concentrations of medicinal herbal extract as decontaminant agent on broiler carcasses. Therefore, a total of twelve broiler carcasses treated with sumac extract (4% and 8% w/v) and rosemary extract (0.3% and 0.5% w/v) and stored at 0±1°C. All treated carcasses were sensory and microbiologically analyzed. It has been found that there were no changes in color and odor of treated carcasses, either by sumac extract (8% w/v) or rosemary extract (0.5% w/v), however, a reduction in the total bacterial count, coliforms count, E. coli count and Staphylococcus spp. count one/two log less than control samples and shelf-life of broiler carcasses was noticed and extended three to six days more than the control. From the present study, it could be concluded that sumac extract (8% w/v) and rosemary extract (0.5% w/v) are effective as broiler meat decontaminant and preservative.

Keywords: Medicinal herbs, rosemary, sumac, decontaminations, broiler carcasses, shelflife
Effect of prenatal and postnatal environmental enrichment on laboratory rats' welfare

Effect of prenatal and postnatal environmental enrichment on laboratory rats' welfare

Authors: Asmaa K. Abdelghany a, Naglaa M. Abdel-Azeem a,*, Mostafa A. S. b, Emeash H. H. a a Department of Hygiene, Management and Zoonoses, Faculty of Veteri...
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Abstract

This work was designed to investigate effects of environmental enrichment during gestation on behaviour, physiology and brain histology of enriched and non-enriched offspring rats. A total of 30 female wistar rats were randomly divided into two groups; control and enriched groups. Offspring from prenatally enriched group were divided after weaning into two groups; one raised under standard condition (enriched group) and the other raised under enriched condition after weaning (E+EC group) from the day 23 to the day 35 postnatal. Observing neonates' behaviour, on the day 36 postnatal, rats subjected to behavioural tests. On the day 42 postnatal, blood samples were collected and brain samples were obtained for histopathology. Behavioural tests revealed significant (P<0.05) increased time spent in open arm, open arm entries and time in center in E+EC group and unprotected stretch attend posture (USAP) were significantly (P<0.01) increased in E+EC group. Freezing time in open field test was significantly (P<0.01) decreased in the enriched group, while grooming frequency was significantly (P<0.05) increased in prenatally and post weaning enriched rats group (E+EC). The corticosterone level was significantly (P<0.05) decreased in prenatally and post weaning enriched rats group (E+EC). The mean of tertiary processes of cytoplasmic processes in cross section of hippocampal region were significantly (P<0.05) increased in prenatally and post weaning enriched rats group (E+EC) group. In conclusion, providing experimental laboratory rats with physical enrichment tools in prenatal and postnatal life can improve their behavioural and physiological status reflecting on their welfare.

Keywords: Enrichment, welfare, rats, corticosterone, histopathology
Effect of calcium soap of palm oil fatty acids on milk composition, ewe and lamb performance in a crossbred sheep

Effect of calcium soap of palm oil fatty acids on milk composition, ewe and lamb performance in a crossbred sheep

Authors: Hanaa H. Abdel-Hakim a, Abdel-Hafeez H. M. b, Samar S. Tawfeek b, *, Hegazy M. A.a a Animal Reproduction Research Institute, El-Ahram, Giza, Egypt b...
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Abstract

This study aimed to evaluate the effect of Ca soap of palm oil fatty acids (CSFA), as a protected fat, on milk composition and the performance of both ewes and lambs. Thirty-three crossbred ewes aged 2-3 years and weighed 41-44 kg were allocated in three unequal groups for A, B &C (10, 12 &11 ewes, respectively). Group A was fed a diet free from protected fat and kept as a control. Group B was fed a diet containing 4 % fat and group C was given a diet containing 8 % fat. The ewes were in the last month of pregnancy and the experiment extended till the end of late lactation (4.5 months). Milk composition was tested, and body change in ewes and nursing lambs was traced. Moreover, the effect of fat addition on dry matter intake of ewes was analyzed. Results revealed a decrease in dry matter intake in the late gestation and an increase in early lactation, with no significant differences in the late lactation. The effect followed the amount of fat added. There was no effect on ewe’s body weight change or lamb’s growth rate from birth to weaning. On milk composition, there was an increase in fat %, higher in group B and total solids%, while percentage of protein was decreased. Concerning fat supplementation, no effect on lactose, urea milk nitrogen, and somatic cell counts was detected. There was a decreased level of solids-not-fat in group B, while group C showed no detectable alterations. In conclusion CSFA supplementation was effective to increase the fat % and yield in milk of sheep especially at the rate of 4% of addition.

Keywords: Calcium soap of palm oils fatty acids, ewe and lamb performance and milk composition
Characterization of E. coli and Salmonella spp. isolates associated with omphalitis in baby chicks

Characterization of E. coli and Salmonella spp. isolates associated with omphalitis in baby chicks

Authors: Azza A. EL-Sawah a, AL Hussien M. Dahshan a, Soad A. Nasef b, El-Shaymaa El-Nahass c, Nayel A. I.d,* a Department of Poultry and Rabbit Diseases, Fa...
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Abstract

Omphalitis is a major cause of increased first week-chick mortality. Omphalitis, navel-yolk sac infection, is a hatchery-born disease, and also known as ‘mushy chick disease’ or ‘navel ill’. It is a common disease of chicks and poults, often artificially hatched chicks, causing high losses in the brooding period, as a bacterium penetrates the porous egg shell. As incubation conditions are suitable for bacterial growth and incubating eggs as well, various bacteria, such as E. coli, staphylococci, Proteus, Clostridium fecali and Pseudomonas may be involved in the yolk sac infection. The present study aimed to determine bacterial causes of omphalitis through isolation and identification of such pathogens. Therefore, samples from 216 yolk sacs were collected from chicks with unabsorbed yolk materials that could even smell putrid. Among those, 196 (90.7%) were positive; 135 (62.5%) harboured single bacterial strains and 61 (28.2%) had mixed infections. The most prevalent single bacterial isolates were E. coli (110 isolates) and P. aeruginosa (11 isolates). Meanwhile, the most predominant mixed bacterial strains were E. coli with Salmonella spp. (16 isolates; 7.4%) and E. coli with P. aeruginosa (13 isolates; 6%). Other mixed infections were found in low percentages. Most E. coli strains were Congo red-positive and non-haemolytic. Different E. coli serogroups were serologically identified including O27 (4 isolates; 20%), O157 (3isolates; 15%), O26 (3 isolates; 15%) and one isolate of each of the following; O78, O6, O125, O44, O15, O115, O25, O168, O112 and O63 (each of 5%). Different Salmonella serogroups were identified including S. cremieu (2 isolates) and one isolate of each of the following S. enteritidis, S. blegdam, S. senftenberg, S. kingston and S. emek. Isolated bacteria differed in susceptibility. The adhesion-encoding genes (crl and fimH genes) of E. coli were detected by cPCR. It has been concluded that chicks with omphalitis harboured different pathogens and they are considered a source of infection during the successive days of life in broiler chickens.

Keywords: Omphalitis, baby chicks, E. coli, Salmonella
Heavy metal residues in local and imported fish in Egypt

Heavy metal residues in local and imported fish in Egypt

Authors: Fatma H.M. Alia, Nasser S. Abdel-Attya,*, Jehan M. M. oufb, Marwa A. S. Moustafac a Food Hygiene Dept., Faculty of Veterinary Medicine, Beni-Suef Uni...
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Abstract

A total of 100 random muscle samples of Oreochromis niloticus, Claries lazera, imported Mugil cephalus and Scomber scombrus fish (25 of each) were collected from different markets in Fayoum City for determination of lead, cadmium, copper and mercury residual levels to ascertain whether these levels exceeded the prescribed legal limits. The mean mercury level in the edible muscles tissues of fish species were ranged from below detectable level to 0.29 ppm in local fish and to 0.28 ppm in imported fish. The residual levels were not exceeded the prescribed legal limits of the European Commission Regulation (EC). The highest mean levels of cadmium were recorded in Mackerel (0.119 ± 0.060, ppm) which exceeded the permitted values stipulated by European Commission (EC). The samples in this study contained copper within of the general guideline limit for copper in food. The highest mean levels of lead were also recorded in Mackerel (0.477±0.073 ppm) which exceeded the permitted value stipulated by European Commission (EC). The results were evaluated according to International standards of WHO and EC. The provisional tolerable weekly intakes used in this study to assess the relative safety of marketed in Fayoum. The public health significance of heavy metal residues in fishes were also discussed.

Keywords: Heavy metals, Oreochromis niloticus, Claries lazera,Mugil cephalus, Scomber scombrus
Organochlorine residues in fishes collected from different water sources in El-Fayoum Governorate

Organochlorine residues in fishes collected from different water sources in El-Fayoum Governorate

Authors: Fatma H.M. Alia, Nasser S. Abdel-Attya,*, Jehan M. M. oufb, Abdel-Latif, A. M.c a Food Hygiene Dept., Faculty of Veterinary Medicine, Beni-Suef Unive...
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Abstract

A total of 120 fish samples were collected from different water sources in El Fayoum Governorate, (Bahr El Banat agricultural drainage, different fish farms and Al Rayaan Lake). and represented by Clarias gariepinus from Bahr El Banat agricultural drainage, different fish farms (15 each), Mugil cephalus from different fish farms and Al Rayaan Lake (15 each), Solea solea (30 samples) and Oreochromis niloticus from Bahr El Banat agricultural drainage, different fish farms and Al Rayaan Lake (10 each). Fourteen organochlorine compounds were analyzed by gas chromatography. Fish samples from Bahr El Banat revealed the highest mean levels of p,p'-DDD, endrin, endosulfan, γ-chlordane, heptachlor and γ-HCH, while fish samples from Al Rayaan Lake have shown the highest mean level of methoxychlor, p,p'-DDT, p,p'- DDE, dieldrin, heptachlor epoxide, δ-HCH and α-HCH. Most of the examined fish samples from different species, are within the maximum residue limits and should not pose a health risk to consumers. The public health hazards were discussed as well as recommendations were done.

Keywords: Organochlorine, Mugil cephalus, Oreochromis niloticus, Clarias gariepinus, Solea solea, Al Rayaan Lakes
Distribution pattern of intestinal helminths in domestic pigeons (Columba livia domestica) and turkeys (Meleagris gallopavo) in Beni-Suef province, Egypt

Distribution pattern of intestinal helminths in domestic pigeons (Columba livia domestica) and turkeys (Meleagris gallopavo) in Beni-Suef province, Egypt

Authors: Khaled Mohamed El-Dakhly a,*, Lilian N. Mahrous a, Gehad A. Mabrouk b a Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef Univers...
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Abstract

Parasitic helminths of pigeons and turkeys are worldwide spread causing mortalities and considerable economic losses. Accordingly, intestinal tracts of domestic pigeons (Columba livia domestica) and domestic turkeys (Meleagris gallopavo) were examined in Beni-Suef province, Egypt to detect their helminth fauna. A total of 740 intestinal samples from pigeons and 100 from turkeys were investigated during the period from June 2015 to May 2016. The overall prevalence of recovered helminths was 11.76% (87/740) in pigeons. The recovered species were identified as two trematodes, 5 cestodes and 4 nematodes. Digeneans were Brachylaima cribbi (1/740; 0.14%) and unidentified Brachylaima sp. (0.14%). Cestodal species were identified as Raillietina echinobothrida (33/740; 4.46%), Raillietina cesticillus (7/740; 0.95%), Raillietina tetragona (7/740; 0.95%), Cotugnia digonopora (5/740; 0.68%) and Hymenolepis carioca (2/740; 0.27%). Among cestodes, R. echinobothrida was the most predominant one. Recovered nematodal species were Ascaridia columbae (22/740; 3%), Subulura brumpti (6/740; 0.81%), Heterakis gallinarum (3/740; 0.41%) and Capillaria spp. (2/740; 0.27%). Ascaridia dissimilis was the only helminth species detected in turkeys (6/100; 6%). The highest prevalence of infection was seen in winter and summer. Tapeworms and round worms were highly prevalent at the summer. The recorded trematodal infection was found in winter. To the best of author's knowledge, adult Brachylaima spp. was first recorded from pigeons in Egypt. Veterinarians and workers of poultry industry must be aware towards the potential role of arthropods as vectors of such helminths among both domestic and wild birds.

Keywords: Pigeons, turkeys, prevalence, helminths, Beni-Suef
Effects of high dietary energy, with high and normal protein levels, on broiler performance and production characteristics

Effects of high dietary energy, with high and normal protein levels, on broiler performance and production characteristics

Authors: Hassan M. Abdel-Hafeez, Elham S. E. Saleh, Samar S. Tawfeek, Ibrahim M. I. Youssef *, Manal B. M. Hemida Department of Nutrition and Clinical Nutrit...
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Abstract

This study was conducted to investigate the effect of high metabolizable energy diets with normal or wide metabolizable energy to crude protein ratios (ME:CP) on the performance, carcass characteristics, body composition and blood parameters of broilers fed from 1 to 56 days of age. The chicks were allotted into 7 groups; one control and 6 tested. The birds were fed starter, grower and finisher diets. The control diet was formulated according to the NRC of poultry (1994) and the other six diets contained three different levels of high energy diets (3275, 3350 & 3425 kcal/kg; one level for each two groups). The first three tested groups were pointed for as the ''normal calorie-protein ratio” groups in which the CP increased in relation to the increased ME, keeping the normal NRC ratio. In the second three tested groups, named “wide calorie-protein ratio” groups, the dietary protein was kept at the NRC levels leading to ratios wider than that of the NRC. Results showed that chicks fed high ME diets with normal energy to protein ratio grew faster, and used feed more efficiently than chicks fed the control diets. However, feeding diets with high ME and normal protein NRC-levels slightly improved the weight and feed conversion. The visible fat and fat retained in the body was higher in all tested groups compared to the control. However, the blood parameters had no significant variations among the treatments, except for ALT which had an increased response to increased dietary energy density. In conclusion, increasing the dietary ME level without increasing the crude protein level provided moderately economic returns and lesser improvement in the performance of broilers. However, increasing of dietary ME with normal ME:CP ratio resulted in increased broiler performance and higher economic return.

Keywords: Broilers, energy, protein, broilers, calorie protein ratio, performance, carcass characteristics.
Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate

Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate

Authors: Nabila A. Ghazya, Mohamed G. Abdelwahabb, Makhareta M.A.M. c, and Abdallah Y.A.d a Department of Serum and Antigens, Veterinary Serum and Vaccine Res...
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Abstract

Corynebacterium pseudotuberculosis vaccine was prepared from a local field isolate. Vaccination of sheep with 50g PLD toxoid and 10 mg bacterin adjuvanted with Montanide oil improved the levels of immune responses of sheep. In many countries, inactivated C. pseudotuberculosis adjuvant vaccines have been used for prevention and control of caseous lymphadenitis in sheep. However, the efficacy was variable. The aim of the present study was directed to prepare and evaluate the potency of an inactivated C. pseudotuberculosis vaccine using Montanide ISA206. Sheep were vaccinated with 1st dose of 2ml containing 10 mg bacterin and 50g toxoid and Montanide ISA 206 oil adjuvant and boostered with the same dose 15 days Apart. Evaluation of post vaccinal cellular immune response with lymphocyte proliferation assay and humoral immune response using ELISA was carried out. Cell mediated immune response of vaccinated sheep reached its peak 0.445 by 1st week post the second vaccination. The level of humoral immune response showed optical density of 1.005 by 1st week post the second vaccination. Challenge test was done in all sheep four weeks after the second dose of vaccination. Three sheep from vaccinated and three sheep from non-vaccinated groups were slaughtered and necropsied 150 days post challenge. The results revealed 75% protection percentage against challenge while unvaccinated challenged sheep showed 9% protection. Statistical analysis indicated that the vaccine assessed a significant level of cellular and humoral immunity.

Keywords: Caseous lymphadenitis, ELISA, Lymphocyte proliferation assay, Montanide A
Using indirect ELISA and PCR for the diagnosis of equine herpes virus-1 (EHV-1) infection in Egypt

Using indirect ELISA and PCR for the diagnosis of equine herpes virus-1 (EHV-1) infection in Egypt

Authors: Fayez A. Salib a, Magda A. Kalad b, Hany M. Hassan c, Samer F. Said c a Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicin...
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Abstract

The present study was carried out for studying the prevalence of equine herpes virus-1 infection among equines in different Governorates of Egypt. One hundred eighty two equines of different ages, sexes, localities and breeds showing signs of fever, respiratory manifestations, abortions, ataxia, dog sitting position, limb edema, foal depression and death were examined for the isolation of EHV-1 on both embryonated chicken eggs and baby hamster kidney (BHK) cell culture, the aborted fetuses were histologically examined for the detection of inclusion bodies. Serum samples were collected to detect immunoglobulin-G specific to EHV-1 by using ELISA. Nasopharyngeal swabs isolated virus and the aborted fetal tissues were tested by PCR using specific primers to prove the infection with EHV-1. The prevalence of EHV-1 infection in the examined animals was recorded as 4.94%. It was prevalent in Cairo, Giza, Kafr Elsheikh, Monofeia, El Sharkia Governorates. The EHV-1 was isolated on the embryo chicken eggs and the pock lesion was appeared on the chorio-allantoic membranes. The cytopathic effects were also observed in tissue cultures. The Liver of aborted fetuses showed necrosis of all hepatic tissue and activation of Kupffer cells with hemosiderin and the detection of eosinophilic intranuclear inclusion bodies. Indirect ELISA could detect IgG in all infected equines (n=9) from which EHV-1 isolated. PCR proved the infection with EHV-1 in the aborted fetal (n=3) tissues and gave similar results by using the horses nasopharyngeal swabs isolated virus where 489 bp PCR products were detected in both. In conclusion, EHV-1 is prevalent in different Governorates in Egypt. EHV-1 infection could be diagnosed by intranuclear eosinophilic inclusion bodies in the aborted fetal tissues. The indirect ELISA could diagnose EHV-1 infection in all ages and sexes groups. PCR applied on aborted fetal tissues is better for the diagnosis of EHV-1 infection than that based on the horse nasopharyngeal swabs isolated virus due to saving time and money.

Keywords: EHV-1, ELISA, PCR, diagnosis, Egypt.
Ultrasonography of the mammary gland in ruminants

Ultrasonography of the mammary gland in ruminants

Authors: Ragab, G. H. a, Seif, M. M.a,*, Qutp, M. M.b a Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Beni-Suef Univers...
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Abstract

Information regarding the use of ultrasonography in the diagnosis of udder and teat affections is scarce in ruminant. The present study was conducted on 30 cows, 20 ewes and 45 does. The surgical affections of udder and teats were recorded in 10 cows, 5 ewes and 15 does. In normal udder, glandular parenchyma on ultrasonographic examination appeared as homogenous and hyperechoic with anechoic alveoli, the milk appeared with high pitched anechoic large areas. While in the teat skin echoed strongly, the middle layer less echogenic than skin in a homogenous, uniform pattern and the teat canal represented by extending area between the two hyper-echoic lines forming anechoic lumen. In multiple abscesses, the glandular parenchyma of udder appeared as multiple hyper-echoic areas surrounded by hypo-echoic margin. In case of super numerary teats, presence of hypo-echoic to an-echoic areas separated by hyper-echoic septa due to each teat had separated milk cisternae. In case of complete teat obstruction, presence of hyper-echoic obstructive mass which present in teat canal. In conclusion, ultrasonography is a good tool for differential diagnosis of different udder and teat affections.

Keywords: ultrasonography, udder, teat, ruminant.
Prevalence of Campylobacter species in milk and some dairy products

Prevalence of Campylobacter species in milk and some dairy products

Authors: A. M. El-Kholy a, A. M. S. Meshref a, A. A. El-Gedawy b and R. M. Esam b a Department of Food Hygiene, Faculty of Veterinary Medicine, Beni-Suef Univ...
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Abstract

Campylobacteriosis is assumed to be mainly a food-borne disease. Also the importance of milk as a source of human Campylobacter enteritis was confirmed by the European Union summary report on food-borne disease outbreaks. Therefore, the present study was undertaken to detect the prevalence of Campylobacters in milk and milk products. A total of 250 samples (100 milk, 50 Domiati cheese, 50 kareish cheese and 50 ice-cream) were collected from the different collection points in El-Minia and Beni-Suef Governorates. The samples were examined by microbiological culture method, and presumptive isolates were further confirmed by genetic amplification (PCR) using specific primers of hippuricase gene.The overall prevalence of Campylobacter species were 13% in raw milk, 52% in kareish cheese, 18% in Domiati cheese and 6% in ice-cream. PCR amplification of hipO gene of isolated C. jejuni from the milk and milk products samples had been shown identical fingerprints with human isolates at 323bp, which indicates the zoonotic hazards of Campylobacter jejuni in Egypt.

Keywords: Campylobacter, milk, dairy products, PCR.

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