Introduction & Aim: The ethanol and aqueous extracts of Alhagi camelorum Fisch. (Fabaceae) whole plant, Scrophularia desertii Del. (Scrophulariaceae) whole plant and ethanol extracts of Echinophora platyloba DC. (Apiaceae) whole plant, Teucrium polium L. (Lamiaceae) whole plant and Berberis integerrima Bunge root brake were investigated for anti-Brucella in-vitro and in-vivo conditions. Experimental: Clinical isolated of Brucella abortus was studied using the disk diffusion method. Brucellosis was induced by repeated injection of Brucella abortus on Balb/C mice. In Balb/C model, we evaluated factors included primary weight/secondary weight ratio (W1/W2), spleen weight, spleen weight/body weight ratio and antibody titer with Wright method. Results & Discussion: The results of disk diffusion approach that the extracts from different plant species studied showed antibacterial activity against B. abortus, with the diameters of zone of inhibition ranging between 1 and 27 mm. Of the plants studied, the most active extracts at the concentration 500 µg/ml were those obtained from aqueous extracts of A. camelorum whole plant and showed strong inhibitory effects (zone of inhibition ≥15 mm) but ethanol extract of S. desertii at the concentration 125 µg/ml showed lowest of inhabitation effect. The bacteria in the study were sensitive to some of antibiotics, erythromycin and streptomycin being the most sensitive (inhibition zone values of 21 and 23 mm, respectively), while was resistant to some of the plant extracts at the concentration 62.5 µg/ml. The best results for W1/W2 were obtained from extract-treated by ethanol extract of B. integrrima root in dose of 400 mg/kg/day and following treated by erythromycin in dose of 400 mg/kg/day. The lowest of Spleen weight were obtained from aqueous extract of A. camelorum and following treated by erythromycin. The best results for Spleen W/Body were obtained from extract-treated by aqueous extract of A. camelorum and following treated by erythromycin in dose of 200 mg/kg/day. Also, the best results for antibody titer with Wright method were obtained from ethanol extract B. integrrima root brake. However, the results of mean of comparison with Duncan's test for antibody titer with Wright method showed no significant difference.