Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 8, Issue 2
Abstract
Background & Aim: Indigenous people of West Africa use the wholeleaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possible mechanisms. Experimental: RT-PCR method was used to investigate regulation of intestinal glucose transporter 2 (GLUT2) and glucagon-like peptide-1 (GLP-1), and pancreatic insulin, L-type voltage-gated calcium channel genes. Insulin exocytosis was also monitored using ELISA method. The kidney sample was investigated for biomarkers of injury (kidney injury molecule-1 (KIM-1) and interleukin-1-β (IL-1β)). Results: GLP-1 up-regulation, GLUT2 down-regulation and increased insulin exocytosis but not increased insulin gene expression was observed in animals after a 3-day culinary exposure to D. Subericarpa leaves. This mechanism may explain hypoglycemia in streptozotocin-induced diabetes in animals in this study. KIM-1 and IL-1-β genes were marked up regulated in normal animals exposed (14-day) to D. Subericarpa. Recommended applications/industries: D. Subericarpa whole leaf contains phytochemicals principles with anti-diabetic potency but may be nephrotoxic. Therefore, for clinical use, selective fractionation of active components from the toxic components is desirable.
Authors and Affiliations
olaposi Omotuyi; Ovuakporie-Uvo Oghale; Idu MacDonald
Keywords
Related Articles
- EP ID EP23543
- DOI -
- Views 344
- Downloads 16
Interactive effects of salicylic acid and cold stress on activities of antioxidant enzymes in Glycyrrhiza glabra L.
Background & Aim: Glycyrrhiza L. (Fabaceae) consists of perennial herbs grow in sandy soils with hard lightness. Three species of the genus grow in Iran, among them G. glabra L. has the widest distribution. It is well kn...
An overview on genus Thymus
The genus Thymus L. belongs to the Lamiaceae family, consist of about 215 species of herbaceous perennials and small shrubs in the world. They originated from Mediterranean region. This genus is presented in Iranian f...
Anti-inflammatory activity of leaf extract of Solanum anomalum
Background & Aim: Solanum anomalum Thonn. ex Schumach. (family Solanaceae) is a shrub growing up to 2 metres tall. The edible fruits are gathered from the wild and consumed locally and the leaves are used locally in t...
Effect of Sucrose Replacement by Stevia as a Non-Nutritive Sweetener and Bulking Compounds on Physiochemical Properties of Foodstuffs
Background & Aim: Changes in lifestyle, decrease in physical exercise along with incidence of obesity, hypertension and cardiovascular diseases indicate a need for low calorie formulations. The sweetness of Stevia, a...
Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats
Background & Aim: Indigenous people of West Africa use the wholeleaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possibl...