Regulation of Intestinal GLP-1 and GLUT2 genes underlie hypoglycemia in Desplatsia subericarpa (Bocq)-Fed Wistar Rats
Journal Title: Journal of Herbal Drugs - Year 2017, Vol 8, Issue 2
Abstract
Background & Aim: Indigenous people of West Africa use the wholeleaf of Desplatsia subericarpa (Bocq) in anti-diabetic soup delicacy. This study was designed to validate the anti-diabetic claims and delineating possible mechanisms. Experimental: RT-PCR method was used to investigate regulation of intestinal glucose transporter 2 (GLUT2) and glucagon-like peptide-1 (GLP-1), and pancreatic insulin, L-type voltage-gated calcium channel genes. Insulin exocytosis was also monitored using ELISA method. The kidney sample was investigated for biomarkers of injury (kidney injury molecule-1 (KIM-1) and interleukin-1-β (IL-1β)). Results: GLP-1 up-regulation, GLUT2 down-regulation and increased insulin exocytosis but not increased insulin gene expression was observed in animals after a 3-day culinary exposure to D. Subericarpa leaves. This mechanism may explain hypoglycemia in streptozotocin-induced diabetes in animals in this study. KIM-1 and IL-1-β genes were marked up regulated in normal animals exposed (14-day) to D. Subericarpa. Recommended applications/industries: D. Subericarpa whole leaf contains phytochemicals principles with anti-diabetic potency but may be nephrotoxic. Therefore, for clinical use, selective fractionation of active components from the toxic components is desirable.
Authors and Affiliations
olaposi Omotuyi; Ovuakporie-Uvo Oghale; Idu MacDonald
Keywords
Related Articles
- EP ID EP23543
- DOI -
- Views 358
- Downloads 16
Dose-dependent effects of Gundelia Tournefortii root extract on meiosis resumption and in vitro maturation in mouse immature oocytes
Background & Aim: Oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. Therefore this study was des...
Comparative polyphenol contents, free radical scavenging properties and effects on adipogenesis of Chorisia Chodatii and Chorisia Speciosa
Background & Aim: Chorisia is an important Bombacaceous plant that is traditionally used for a variety of ailments. Due to its richness in several bioactive phytocompounds, some Chorisia species showed a wide range of...
Regional variations of antioxidant capacity and phenolic properties in the Iranian jujube collection
Background & Aim: Fruits from the jujube plant (Ziziphus jujuba) possess health-promoting effects and medicinal properties. Jujube has a long history of usage as a remedy in Iranian traditional medicine and is recomme...
The effect of Kardeh (Biarum Bovei) Hydro-alcoholic extract on pain threshold in STZ induced diabetic rats
Background & Aim: Diabetes mellitus in long-term through various means such as augmented oxidative stress is associated with disturbances such as pain and cognition in humans and laboratory animals. According to the evid...
Chemical compositions of the essential oil of Gundelia tournefortii L. (Asteraceae) from Central Zagros, Iran
Background & Aim: Gundelia tournefortii L. (Tumbleweed) has been identified as one of the well-known and widely distributed plants with various applications in some of Asian and North African countries. It belongs to...