• Journal title: Journal of Advanced Biotechnology and Experimental Therapeutics
• ISSN: 2616-4760 (online)
• Publisher: Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
• Country of publisher: bangladesh
• Date added to EuroPub: 2019/Feb/20

• LCC Subject Category: Biological Sciences, Genetics, Microbiology, Pharmacology, Toxicology
• Publisher's keywords: TLQP-21, SH-SY5Y, VGF, Phosphoprotein, Gel stain
• Language of fulltext: english
• Full-text formats available: PDF

Md. Shamim Akhter, Jesús R. Requena

• Double blind peer review
• Editorial Board
• Aims and scope
• Instructions for authors
• Time From Submission to Publication: 5

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VGF (non-acronymic), belonging to a large granin family, gives rise to a number of bioactive peptides by proteolysis and exert an extensive array of biological effects on energy metabolism, pain modulation, gastric secretion function, reproduction, mood regulation, and, diabetes. Among VGF-derived peptides, TLQP-21 (The first four amino acids, in short TLQP (Thr-Leu-Gln-Pro) generalizes the nomenclature of the peptide by its length) is the most studied although little is known yet about downstream molecular mechanisms of action of VGF-derived peptides like TLQP-21. So here as a preliminary analysis, total protein expression was carried out in addition to the phosphoproteomic study of SH-SY5Y cells treated with TLQP-21, using the same cell extracts. Comparison of simple 1D SDSPAGE gels stained with SYPRO® Ruby protein gel stain was carried out to assess whether changes in protein expression could be seen even at such low separation resolution. Expression of several proteins most likely Microtubule-associated protein 1B (MW 271 kDa), Tubulin beta chain (MW 57), Tubulin beta- 4B chain (MW 50), Alpha-2-macroglobulin (MW 163), etc. in TLQP-21 treated and control samples was found significantly different, indicating that the peptide TLQP-21 exerts biological effects on SH-SY5Y cells. Further studies are required to validate the identity of the modulated proteins, obtained from mass spectrometry. Identification of modulated proteins after TLQP-21 treatment would open new avenues to discover the molecular mechanisms of its physiological and pharmacological state.