A protein isolation method for western blot to study histones with an internal control protein
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2018, Vol 1, Issue 1
Abstract
Histone modification is one of the attractive targets for epigenetic studies. However, current methods to extract chromatin-associated proteins for western blot of histone modification have some weaknesses such as the loss of housekeeping proteins. In this study, we are presenting a simple method to isolate nuclear protein for studying histone modification by immunoblotting with housekeeping proteins. This method provided high protein concentration from minute tissue samples and importantly, it allowed us to detect acetylated histones together with internal control proteins such as β actin.
Authors and Affiliations
Dinh Toi Chu, Yang Tao
Keywords
Related Articles
- EP ID EP36333
- DOI -
- Views 440
- Downloads 0
Ocimum herb species: a potential treatment strategy for diabetic kidney disease
Ocimum genus most commonly termed as Tulsi, an aromatic plants belongs to family Lamiaceae. In addition to anti bacterial activity, anti-oxidant, anti diabetic, cardio protective, neuroprotective, radio-protective, ant...
Insilico analysis of γ- aminobutyric acid transaminase (GABA-T) of Brassica napus (Rape)
γ- aminobutyric acid (GABA) is an essential metabolite which plays a crucial role in signal transmission, stress metabolism, and some other activities also reported. Although the actual function of GABA shunt is not cl...
Role of auxin and nitric oxide on growth and development of lateral root of plants: possible involvement of exogenously induced Phot1
Development of root architecture including lateral root formation is a critical event for successful growth of plants. Auxin promotes the production of nitric oxide (NO) in roots, which is required for auxin-mediated r...
Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells
Wharton’s jelly (WJ) is a gelatinous tissue exists in the umbilical cord contains stromal cells, termed as mesenchymal stromal cells (MSCs). Current study designed to characterize the stem cell population both within t...
Choice of samples in extracellular microRNA research: Which fraction is better- exosomal or nonexosomal?
Abstract: MicroRNAs (miRNAs) are a species of noncoding RNA that post-transcriptionally regulates the expression of target mRNAs via degradation or translational repression. Recently, miRNAs in biofluids got extraordinar...