Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet ‘white’)
Journal Title: Journal of Advanced Biotechnology and Experimental Therapeutics - Year 2018, Vol 1, Issue 2
Abstract
Seeds of white hyacinth bean available in Khulna, Bangladesh were investigated to assess bioactivities such as antibacterial and cytotoxic properties. For the evaluation of bioactivities solvent extraction was performed by using 50% ethanol and 50% methanol. In the present study, in vitro antibacterial screening was done by the method known as disc diffusion assay. Additionally, the cytotoxic activity was screened by using brine shrimp lethality bioassay. Both the extracts exhibited excellent activity against both gram positive and gram negative bacteria. For ethanolic extract of white hyacinth bean, maximum zone of inhibition was found 17.75 mm at 1000 μg/disc against Staphylococcus epidermidis and 16.75 mm against Staphylococcus aureus. On the other hand, for methanolic extract the maximum zone of inhibition was found as 17.25 mm for 1000 μg/disc against Escherichia coli and 16.75 mm against Pseudomonas aeruginosa. In comparison to ethanolic extract, methanolic extract was found to be more active against all tested microorganisms. Compared to vincristine sulphate (with LC50 of 0.99 μg/ml) both ethanolic and methanolic extracts of hyacinth bean seeds showed toxicity lower than 100 μg/ml and they were 34.67 μg/ml and 45.5 μg/ml, respectively. The LC50 values suggest moderate cytotoxicity of the tested samples. The experimental findings could be correlated with the traditional medicinal uses of the seeds of this plant and showed the rational for further investigation which would be required for isolating the possible bioactive constituents responsible for such activities.
Authors and Affiliations
Sheikh Ashikur Rahman, Md. Shamim Akhter
Keywords
Related Articles
- EP ID EP36339
- DOI -
- Views 472
- Downloads 0
Ocimum herb species: a potential treatment strategy for diabetic kidney disease
Ocimum genus most commonly termed as Tulsi, an aromatic plants belongs to family Lamiaceae. In addition to anti bacterial activity, anti-oxidant, anti diabetic, cardio protective, neuroprotective, radio-protective, ant...
Spectrochemical characterization of Vero cell line against PPR virus infection
An in vitro virus infectivity assay based on the newly introduced spectrochemical method was performed using Peste des Petits Ruminants (PPR) virus infected Vero cell line as a model infection. Herein, the mitochondria...
Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice.
Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testic...
Lipopolysaccharide tolerance attenuates inflammatory responses by increasing hemeoxygenase 1 and tristetraprolin expression in Raw264.7 macrophages
Inflammation is associated with the progression of a variety of diseases. Lipopolysaccharides (LPS) tolerance is recognized to reduce proinflammatory responses. Heme oxygenase1 (HO1) and tristetraprolin (TTP) are induc...
Isolation of stem cell populations from wharton’s jelly sections of umbilical cord and comparison analysis with cord blood stem cells
Wharton’s jelly (WJ) is a gelatinous tissue exists in the umbilical cord contains stromal cells, termed as mesenchymal stromal cells (MSCs). Current study designed to characterize the stem cell population both within t...