苦杏仁中苦杏仁酶的闪式提取工艺研究
Journal Title: 现代药物与临床 - Year 2011, Vol 26, Issue 2
Abstract
目的 建立苦杏仁中苦杏仁酶的闪式提取工艺，并对其工艺条件进行优化。方法 以苦杏仁酶总活力为指标，考察了氨水浓度、提取液用量、提取时间、中和终点pH值和沉淀时乙醇体积分数对闪式提取工艺的影响；采用二硝基水杨酸（DNS）法测定苦杏仁酶的比活力，并计算其总活力。结果 苦杏仁酶的最佳提取工艺条件为：取50.0 g脱脂苦杏仁粉，使用500 mL 62.5 mmol/L预冷氨水提取120 s 后，残渣再使用450 mL提取液提取90 s，滤过，滤液用36%醋酸调pH 4.0，滤去杂蛋白后加入4倍量乙醇，沉淀苦杏仁酶。结论 采用闪式提取法改进了苦杏仁酶的提取工艺，并对该工艺进行了优化，得出了较优的闪式提取条件。该工艺具有周期短、操作简便、酶活力高等优点，提取效果优于传统方法。
Authors and Affiliations
Bing HAN , Li-sheng ZHENG , Xiang-qian LIU , Qin-peng ZOU
Keywords
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