16S RRNA CHARACTERIZATION OF CELLULASE PRODUCING BACILLUS CEREUS PREVALENT IN DECAYING AGRO WASTE.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 9
Abstract
The present study was undertaken to isolate and screen an efficient cellulase producing bacteria from decaying agricultural waste. Bacterial cultures were isolated from agricultural wastes produced in the Bihar region. The preliminary isolation was done on nutrient agar media. The isolates obtained were screened for cellulase production by plating on CMC and Congo red agar media to observe the zone of cellulose hydrolysis. Out of 25 isolates 14 strains showed cellulase production on CMC and congo red agar. Further these isolates were used for crude enzyme production. The carboxy methyl cellulase (CMCase) activity was assayed using a method described by Miller, with some modifications. S18 bacterial isolate which showed maximum enzyme activity (0.420 IU/ml/min) was further subjected to biochemical and molecular characterization. On the basis of molecular characterization, the isolates S18 was identified as Bacillus cereus. Therefore, the cellulase producing Bacillus cereus isolated and identified in this study from Bihar region have potential for further use and study, such as to isolate novel cellulases with unique characteristics. Also the enzymatic degradation of cellulose agrowaste produced in this region has been suggested as a possible alternative for the conversion of lignocelluloses substrate into fermentable sugars.
Authors and Affiliations
Jaya Philip, Tanuja .
Keywords
Related Articles
- EP ID EP400735
- DOI 10.21474/IJAR01/7699
- Views 76
- Downloads 0
OCCUPATIONAL HEALTH HAZARDS AND THEIR CAUSES AMONG WOMEN IN INDIAN AGRICULTURE.
Women?s contribution to agriculture, whether it is in subsistence farming or commercial agriculture, when measured in number of tasks performed and time spent, is greater than men. The proportion of women in agricultural...
SOME FEATURES OF EXISTENTIAL SENTENCES IN LANGUAGES BELONGING TO DIFFERENT LANGUAGE SYSTEMS.
The article deals with the main features of existential sentences in English, Azerbaijani and Russian languages. The author makes such an inference that the existential sentences are of universal nature from semantic poi...
THE EFFECT OF DIFFERENT SUPERSTRUCTURE MATERIALS OF IMPLANT SUPPORTED RESTORATION. A SYSTEMATIC REVIEW.
Objectives: The purpose of this review was to recognize the biological complication of implant superstructure materials in comparative to alveolar bone loss around superstructure. Methodology: A search on the electronic...
THERAPEUTIC USE OF BOTULINUM TOXIN IN DENTISTRY.
Botulinum toxin is a versatile therapeutic agent that has become the most successful non-surgical method in Brazil for being effective in various dental treatments. The main purpose of this study is the therapeutic use o...
ADOLESCENT BOYS IN IRELAND: ALCOHOL, CANNABIS, SEXUAL ORIENTATION AND MOOD.
A questionnaire survey of 303 adolescent boys in years 4 (Transition Year), 5 and 6 in secondary schools in counties in the east, southeast and south of Ireland was conducted during the first term of the 2017-18 academic...