QTLs Mapping of Morpho-Physiological Traits of Flag Leaf in Steptoe × Morex Doubled Haploid Lines of Barley in Normal and Salinity Stress Conditions
Journal Title: International Journal of Farming and Allied Sciences - Year 2016, Vol 5, Issue 5
Abstract
Salt tolerance is a complex trait that involves multiple physiological and biochemical mechanisms and numerous genes. The identification of genes whose expression enables plants to tolerance salt stress is essential for breeding programs. In order to a doubled haploid line (DHL) population with 72 lines derived from a cross of ‘Steptoe/Morex’ was used to identify quantitative trait loci (QTL) of morpho-physiological traits in barley (Hordeum vulgar L.). This experiment was carried out in the Research Farm, College of Agriculture, University of Zabol, Zabol, Iran, in growing year 2012. It was arranged as in two randomized complete block design (RCBD) with two replicates and two normal and salinity stress conditions. Morpho-physiological traits of DHLs were measured in two environments. Analysis of variance (ANOVA) results showed that there were significant differences among the lines. Transgressive segregation in both directions was significant for flag leaf length, flag leaf width, flag leaf area and chlorophyll contents. The strongest positive correlations were observed between flag leaf length and flag leaf width (r = 0.994**). QTL analysis was conducted by composite interval mapping (CIM) method separately for each trait in each conditions and mean of two conditions. Twenty-nine QTLs were identified for the studied traits. Phenotypic variance explained by these QTLs variable from 8.66 to 29.94. Highest LOD scores were obtained for flag leaf area (QFla2n) on 2H chromosome in normal conditions.
Authors and Affiliations
Habibeh Shahraki, Barat Ali Fakheri
Keywords
Related Articles
- EP ID EP32862
- DOI -
- Views 309
- Downloads 0
Response of straw and grain protein and nitrogen use efficiency in different plant densities and different nitrogen levels in maize (Zea Mays L.) hybrid 704
The objective of this study was to evaluate the effect of plant population and nitrogen rate on yield and straw and grain protein and nitrogen use efficiency of corn (Zea Mays L, hybrid 704) in 2008 at Mamasani region,...
Evaluation of Salt Tolerance on Germination Stage and Morphological Characteristics of Some Medicinal Plants Artichoke, Flax, Safflower and Coneflower
In order to examine seed germination responses of four medicinal plant coneflower, safflower, flax and artichoke to different levels of salinity, an experiment was performed in the Faculty of Agriculture, Ferdowsi Univ...
Morphological traits of maize affected by seed vigor and water limitation
An experiment was conducted as factorial based on RCB design with three replicates in 2010 to investigate the effects of seed vigor (three seed lots with acceptable normal germinations of 98%, 92% and 88%) and differen...
The Study Effects of Heavy Metals on Germination Characteristics and Proline Content of Triticale (Triticoseale Wittmack)
In order to study, the effects of copper sulfate, cadmium nitrate and lead nitrate on germination characteristics and proline content of Triticoseale Wittmack an experiment in factorial-laid out complete randomized des...
Comparison of molecular markers ( RAPD, ISSR) for determination genetic differences two of Crocus genus
In this study, the genetic diversity of 25 Iranian Crocus genotypes from three species (C. speciosus, C. cancellatus and C. sativus) were evaluated using ISSR and RAPD molecular markers. 19 RAPD primers were selected t...