Comparison of molecular markers ( RAPD, ISSR) for determination genetic differences two of Crocus genus
Journal Title: International Journal of Farming and Allied Sciences - Year 2015, Vol 4, Issue 5
Abstract
In this study, the genetic diversity of 25 Iranian Crocus genotypes from three species (C. speciosus, C. cancellatus and C. sativus) were evaluated using ISSR and RAPD molecular markers. 19 RAPD primers were selected to produce polymorphic and high resolution bands. In total, 261 DNA fragments were polymorphic and 13 ISSR primers were selected in this study. In total, 161 DNA fragments were obtained, from which 153 were polymorphic. Based on the Mantel test, significant correlation was found between similarity matrixes of RAPD and ISSR markers (r = 0.34). Abbreviations SSR Simple Sequence Repeat UPGMA Un-weighted Pair Group Method with Arithmetic mean PIC Polymorphism Information Content RAPD Random Amplified Polymorphic DNA ISSR Inter-simple Sequence Repeat DART Diversity Arrays Technology
Authors and Affiliations
Bita Khansarinejad, Mohammad Reza Hassandokht, Vahideh Nazeri and Aboozar Soorni
Keywords
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