5α-Oleandrin reduce Bcl-2 protein and increase Bax protein expression on Hela cervical cancer cell
Journal Title: Universa Medicina - Year 2017, Vol 36, Issue 2
Abstract
BACKGROUND The leaves of Nerium indicum Mill. have been utilized traditionally to cure cancer. By using Bioassay guided extraction and isolation method, three compounds (NiO-1, NiO-2, NiO-3) were isolated from an active fraction of the CHCl3 extract of N. indicum leaves. NiO-2 identified as 5α- oleandrin is the best cytotoxic compound on HeLa cervical cancer cell in vitro among the other two. However mechanism of action of the compound hasn’t been evaluated yet. The aims of this study were to determine the mechanism of action at molecular level. METHODS The action mechanism of 5α-oleandrin on HeLa cervical cancer cells was analyzed by staining the cells with Hoechst 33342; the agarose gel electrophoresis was aimed to determine the DNA fragmentation and the western blotting was aimed to determine the Bcl-2 and Bax protein expression. RESULTS Incubation of HeLa cervical cancer cell with 5α-oleandrin at the concentration 3,47x10-4 mM (24 hours) followed by staining with Hoechst 33342, a broken up light blue color of nucleus was observed (compared with intensive color of untreated control). By gel electrophoresis (at the same concentration of the tested compound), a smear band at about 200 bp was observed. In addition, cells treated with 5α-oleandrin displayed a decreasing of the Bcl-2 protein expression and increasing of the Bax protein expression CONCLUSION 5α-oleandrin induced HeLa cervical cancer cells DNA fragmentation observed by the presence of a smear band at about 200 bp indicative the apoptotic occurrence. 5α-oleandrin induces apoptosis by reducing the Bcl-2 protein expression but the Bax protein expression increases.
Authors and Affiliations
Mae Sri Hartati Wahyuningsih, Sofia Mubarika, Ibnu G. Ganjar, Subagus Wahyuono, Tatsuo Takeya
Keywords
Related Articles
- EP ID EP376433
- DOI 10.18051/UnivMed.2017.v36.102-109
- Views 330
- Downloads 0
Age-related changes of malondialdehyde, body weight and organ weight in male mice
Introduction Aging is characterized by gradual impairment in all physiological functions. Increases in free radicals and changes in organ morphology occur with aging. The purpose of this study was to determine age-relate...
Tumor necrosis factor-α-activated mesenchymal stem cells accelerate wound healing through vascular endothelial growth factor regulation in rats
Background Wounds are areas of physical or thermal damage of the epithelial layer of skin or mucosa. The wound healing process consists of hemostasis, inflammation, proliferation, and remodeling. Mesenchymal stem cells (...
Glycemic control for prevention vascular complication in diabetic patient
Patients with type 1 diabetes (T1D) and type 2 diabetes (T2D) show an increased risk of vascular complications such as cardiovascular disease and an increased mortality rate resulting in a decrease in life expectancy of...
Underweight increases the risk of pulmonary tuberculosis in adult
Background Tuberculosis (TB) is a leading cause of morbidity and mortality, especially in middle- and low-income countries. The risk of developing TB may be related to nutritional status. Socioeconomic and behavioral fac...
Hormonal contraception increases risk of breast tumor based on clinical breast examination among adult women
Background In Indonesia, cancer prevalence according to the Basic Health Research 2013 was 1.4 per 1000 inhabitants and the most common cancer in hospitalized patients in 2010 was breast cancer (28.7%). Hormonal contrace...