Stability indicating simultaneous validation of Paracetamol, Phenylpropanolamine and Triprolidine with forced degradation behavior study by RP-HPLC in pharmaceutical dosage form

Stability indicating simultaneous validation of Paracetamol, Phenylpropanolamine and Triprolidine with forced degradation behavior study by RP-HPLC in pharmaceutical dosage form

Journal

  • Journal title: Asian Pacific Journal of Health Sciences
  • ISSN: 2350-0964 (print) 2349-0659 (online)
  • Publisher: IJPMR Publishing Press, India.
  • Country of publisher: india
  • Date added to EuroPub: 2019/Sep/29

Subject and more

  • LCC Subject Category: Medicine, Health and Wellness
  • Publisher's keywords: HPLC, Paracetamol, Phenylpropanolamine, Triprolidine, Validation, Forced degradation.
  • Language of fulltext: english
  • Full-text formats available: PDF
  • Time From Submission to Publication: 6

AUTHORS

    Anurag Mishra

FULL TEXT

To download PDF files Login to your Account.

ABSTRACT

A simple, precise, and accurate RP-HPLC method has been developed and validated for the simultaneous assay of Paracetamol, Phenylpropanolamine and Triprolidinein tablet dosage form. Isocratic RP-HPLC method was developed on BDS hypersil C18, (250mm×4.6mm internal diameter, 5µ particle size) using mobile phase as Water (pH-4.0): Methanol (70:30v/v) at a flow rate of 1.0 mL/min and the detection was carried out at 220nm using tunable absorbance detector (Waters 486). Forced degradation study was carried out by acid degradation, base degradation, thermal degradation, oxidation of the drug. The method was validated for linearity, precision, accuracy and robustness. It was found to ideally resolve the peaks with retention time (RT) 4.053min,5.603min and 11.083 min for Phenylpropanolamine, Paracetamol and Triprolidine respectively.The method was found to be linear in the concentration range of 50-150𝜇g/mL with correlation coefficient of 0.9994 for Paracetamol,2.5-7.5𝜇g/mL with correlation coefficient of 0.9995 for Phenylpropanolamineand 2.5-7.5𝜇g/mLwith correlation coefficient of 0.9992 for Triprolidine. Degradation products produced as a result of stress studies did not interfere with the detection of Paracetamol, Phenylpropanolamine and Triprolidine; therefore, the assay can be considered to be stability indicating.

About Europub

EuroPub is a comprehensive, multipurpose database covering scholarly literature, with indexed records from active, authoritative journals, and indexes articles from journals all over the world. The result is an exhaustive database that assists research in every field. Easy access to a vast database at one place, reduces searching and data reviewing time considerably and helps authors in preparing new articles to a great extent. EuroPub aims at increasing the visibility of open access scholarly journals, thereby promoting their increased usage and impact.