Stability indicating simultaneous validation of Paracetamol, Phenylpropanolamine and Triprolidine with forced degradation behavior study by RP-HPLC in pharmaceutical dosage form
Journal Title: Asian Pacific Journal of Health Sciences - Year 2018, Vol 5, Issue 3
Abstract
A simple, precise, and accurate RP-HPLC method has been developed and validated for the simultaneous assay of Paracetamol, Phenylpropanolamine and Triprolidinein tablet dosage form. Isocratic RP-HPLC method was developed on BDS hypersil C18, (250mmร4.6mm internal diameter, 5ยต particle size) using mobile phase as Water (pH-4.0): Methanol (70:30v/v) at a flow rate of 1.0 mL/min and the detection was carried out at 220nm using tunable absorbance detector (Waters 486). Forced degradation study was carried out by acid degradation, base degradation, thermal degradation, oxidation of the drug. The method was validated for linearity, precision, accuracy and robustness. It was found to ideally resolve the peaks with retention time (RT) 4.053min,5.603min and 11.083 min for Phenylpropanolamine, Paracetamol and Triprolidine respectively.The method was found to be linear in the concentration range of 50-150๐g/mL with correlation coefficient of 0.9994 for Paracetamol,2.5-7.5๐g/mL with correlation coefficient of 0.9995 for Phenylpropanolamineand 2.5-7.5๐g/mLwith correlation coefficient of 0.9992 for Triprolidine. Degradation products produced as a result of stress studies did not interfere with the detection of Paracetamol, Phenylpropanolamine and Triprolidine; therefore, the assay can be considered to be stability indicating.
Authors and Affiliations
Anurag Mishra
Keywords
Related Articles
- EP ID EP516667
- DOI 10.21276/apjhs.2018.5.3.7
- Views 351
- Downloads 0
Prevalence of cognitive impairment in urban elderly population and its association with their physical activity
Background: Cognitive deterioration is a part of normal physiological aging, but not everyone goes through a cognitive impairment. Cognitive impairment (CI) ranges from mild cognitive impairment (MCI) to dementia and Alz...
Clinical - epidemiological profile and diagnosis of Influenza A H1N1 cases by real time RT-PCR at a tertiary care institute of India: the war is not over yet
Background: Influenza A virus is a common human pathogen that has caused serious respiratory illness and death over the past century. In April 2009, WHO declared pandemic influenza A H1N1 public health emergency of inter...
Attenuation of hypertensive response with esmolol and labetalol in low doses in orotrachealintubation: A comparative study
Background: The golden standard is rigid laryngoscopy and tracheal intubation in airway management. Aim: To study efficacy in stress response prevention on laryngoscopy and intubation and to study hemodynamic parameters...
Assessing the relationship between HIV infection and cervical cancer at Ndola Central Hospital from 2013 to 2014
Background: Cervical cancer is the second highest cause of cancer related death worldwide, the highest in Africa and also the most frequent cancer in Zambian women. The incidence is high in human immunodeficiency virus p...
Diagnostic accuracy of fine needle aspiration cytology for thyroid lesions in correlation to histopathology
Background: Thyroid enlargement is a common problem and causes various pressure symptoms. Majority of swellings does not require surgery. Fine needle aspiration cytology (FNAC) is considered the gold standard diagnostic...