Risk assessment of Horizontal Gene Transfer in bacterial contaminants of sugarcane in vitro cultures
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 2
Abstract
The horizontal gene transfer is a phenomenon that must be taken into account when performing risk evaluation of genetically modified plants, because these ones could transmit by this method the artificially incorporated transgenes to other organisms with those they are in strait contact in the environment and affect them somehow. The aim of this study was to evaluate the risks of horizontal gene transfer in bacterial contaminant species of in v it r o culture of non-transgenic sugarcane (Saccharum spp. hybrid), which in some cases coincide with endophytic species and/or residents of the rhizosphere of transgenic plants. It was determined the capacity of natural transformation of fourteen bacterial strains with a plasmid containing genes that confer resistance to antibiotics Ampicillin, Streptomycin and Spectinomycin, used as a vector for plant genetic transformation, and from the calculation of transformation frequencies of each species the risks were assessed. Transformed and non transformed bacteria were obtained. The lower frequency was obtained for Escherichia coli (0.09 x 10 -9 ) and the highest for Pseudomonas stutzeri (266.6 x 10 -9 ). The fact that some of the studied bacteria were naturally transformable is a risk factor for them, and it can be used as a model to study horizontal gene transfer, in case to be found as endophytic or associated with the rhizosphere of plants that have been genetically modified when conducting risks assessments to such plants.
Authors and Affiliations
Leonardo J . Moreno-Bermúdez, José Machado-Rodríguez, Yelenys Alvarado-Capó, Mileidy Cruz- Martín
Keywords
Related Articles
- EP ID EP683715
- DOI -
- Views 206
- Downloads 0
Damage to membrane and cellular respiration of Passalora fulva by extracts of leaves of Citrus aurantiifolia
The foliar mold of tomato (Solanum lycopersicum L.), produced by Passalora fulva, constitutes an important phytosanitary problem that affects the production of tomato in greenhouse. The use of plant extracts could cont...
Micropropagation of Stevia rebaudiana Bertoni plants from ex vitro explants
Stevia rebaudiana Bertoni is a species recognized worldwide for its medicinal properties. The objective of this work was to micropropagate Stevia plants from ex vitro explants. Shoots of plants of this specie growing at...
Physiological indices associated with the growth of varieties of potato obtained by biotechnological methods
The aim of this work was to evaluate physiological indexes associated with growth of potatoes varieties (Solanum tuberosum L.) micropropagated using biotechnological methods. Minitubers with 10-15 grams of ‘Grettel’, ‘...
In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa...
Protocol for the isolation of intact chloroplasts from leaves Musa spp. obtained by in vitro culture and evaluation of photosynthetic activity
Chloroplasts are organelles of plant cells where photosynthesis takes place by converting light energy into chemical energy (ATP) and generating reducting power (NADPH). Isolation of chloroplasts and evaluation of phot...