In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 1
Abstract
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa (Ekman & Urban) it is an endemic species of Cuba in extinction danger due to the urbanization of their natural habitat, which have been reduced to a few hectares. The aim of this work was to achieve the establishment in vitro of this species. Seeds and young branches were collected starting from plants in its natural habitat. The effect of three concentrations of NaOCl (2.0, 2.5, 3.0%) during 20 minutes in the disinfection of the seeds was studied. For the disinfection of the buds a treatment with alcohol to 70% was used during two minutes previously to the disinfection with NaOCl. The effect of three concentrations of this NaOCl was studied (1.0, 2.0, 3.0%) during 10 minutes. The present microbiota was characterized in the contaminated branch. The 100% of disinfection of the seeds was achieved in all the treatments studied. The bigger explants percentage free of contaminant (88.6%) was obtained in the treatment with 3% of NaOCl. However the biggest percentage of survival (45.7%) was obtained when 2.0% of NaOCl was used. The establishment in vitro of the species was achieved starting from seeds and buds collected of field plants.
Authors and Affiliations
Elisa Quiala, Grecia Montalvo , Jesús Matos, Reynaldo Mederos , Yelenys Alvarado, Manuel de Feria, Maité Chávez
Keywords
Related Articles
- EP ID EP684315
- DOI -
- Views 130
- Downloads 0
Morphological characterization in nursery of Coffea arabica L. cv. Caturra rojo J-884 plants obtained by somatic embryogenesis
The culture of plant cells and tissues plays an important role in agricultural biotechnology, somatic embryogenesis as a method of plant regeneration has been achieved in a large number of families and species includin...
Work strategy for the prevention and control of bacterial contamination in the micropropagation of sugarcane
The most important losses in labs are caused by microbial contamination. Though several methods have been assayed and put into practice to eliminate or to diminish bacterial contamination of in vitro culture in cells and...
Effect of the physical state of the culture medium and the number of subcultures in the in vitro multiplication phase of plants of Bambusa vulgaris var. vulgaris. Schrad. ex Wendl.
Bambusa vulgaris var. vulgaris. Scharde ex Wendl. is a fast growing multi-species which provides benefits to agricultural crops. It has been used in plantations and agroforestry systems. In vitro propagation of this sp...
In vitro propagation of Vriesea in immersion systems temporary
Bromeliads are ornamentals plants very attractive due to leaves coloration and the beautiful inflorescences. Vrieseas is a genus of a great commercial interest among them. Traditional propagation techniques are limited...
Effect of foliar fertilization on Ananas comosus L. Merr. cv. ‘Cayena lisa’ acclimatization
The low survival and slow growth of in vitro pineapple plants (Ananas comosus L. Merr.) in acclimatization stage limit the use of biotechnological techniques for it propagation. The aim of this study was to determine t...