Work strategy for the prevention and control of bacterial contamination in the micropropagation of sugarcane
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 2
Abstract
The most important losses in labs are caused by microbial contamination. Though several methods have been assayed and put into practice to eliminate or to diminish bacterial contamination of in vitro culture in cells and plant tissues, none has been completely effective. In the present research the aim was to find effective methods to prevent and to control the bacterial contamination of in vitro culture of sugarcane. The culture media for the in vitro plant multiplication was modified by adding nutritive substances in order to evaluate its effect on bacterial contamination percent and its phytotoxicity on in vitro plants. It was also evaluated the effect of modification of the initial pH of the in vitro plant culture medium and bacterial contaminants. It was proved that it is possible, without damaging the in vitro plant, to increase the growing of bacterial contaminants by adding coconut water (100.0 ml.l-1) or yeast extract (250.0 mg.l-1) to the culture medium of multiplication of the sugarcane. The use of the initial pH 6.5 favors the early appearance of bacterial contaminants detecting them since the first 24 hours without a negative effect to the in vitro plant. Evaluating these results a working strategy was set for the prevention and control of bacterial contamination in sugarcane micropropagation by applying early detection methods.
Authors and Affiliations
Yelenys Alvarado-Capó, Mileidy Cruz-Martín, Nayanci Portal González, Leyanis García-Águila, Marisol Freire-Seijo, Elisa Quiala, Rafael Gómez-Kosky
Keywords
Related Articles
- EP ID EP697879
- DOI -
- Views 73
- Downloads 0
Effect of temperature, pH and detergents on the antifungal activity of bacterial culture filtrates against Mycosphaerella fijiensis
The bacteria associated to crops have been studied as potential biocontrol agents. However, few investigations on the interaction Musa spp. - Mycosphaerella fijiensis-Musa associated bacteria have been developed. Conse...
Study on the effectiveness of RIZOBAC in the process of calllogenesis in potato var. Desiree
This experiment was carried out in a plant biotechnology laboratory at Havana Agronomy University (UNAH), evaluating the effects of different concentrations of bacterial preparations (RIZOBAC) on the obtention of fresh m...
Effect of explant density and volume of culture medium on the in vitro propagation of the hybrid cultivar 'FHIA-21' (AAAB) in Temporary Immersion Systems
The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. T...
Biochemical and histological characterization of Agave fourcroydes Lem. plants in vitro acclimatized
Henequen (Agave fourcroydes Lem.) is a representative crop of the province of Matanzas, Cuba, which has been exploited extensively for the quality of its fibers. The present work aimed to characterize from the biochemi...
In vitro multiplication of Zantedeschia spp. variety Treasure in Temporary Immersion Systems
Callas (Zantedeschia spp.) are plants from Araceae family of a great interest as ornamental plants in pots and as cut flowers. Although they have been propagated by in vitro culture, there are few scientific studies on...