Plant regeneration of five cultivars of Phaseolus vulgaris L. via direct organogenesis
Journal Title: Biotecnología Vegetal - Year 2012, Vol 12, Issue 1
Abstract
The beans are an important source for human consumption in Cuba due their protein content. For this reason; the search for cultivars with greater potential is a prominent challenge in this specie. Tissue culture in Phaseolus vulgaris L. is very difficult, among other things, due to its genotype-dependent condition. The aim of this study was to evaluate the regeneration of plants via direct organogenesis in five genotypes o f P. vulgaris. The genotypes studied were ‘ICA Pijao’, ‘BAT 93’, ‘BAT 482’, ‘BAT 304’ and ‘CIAP7247F’. The investigation showed that genotypes ‘BAT 304’ and ‘Pijao ICA’ had the highest values of explants with multiple buds induction. In the variable number of multiple buds per explant, the highest values were obtained in ‘CIAP7247F’ and ‘BAT 482’ genotypes. In ‘BAT 93’ callus formation was so profuse that reduced the number of explants with buds induced. Plant regeneration was another variable that differed in the genotypes studied. ‘CIAP7247F’ showed the greater number of shoots per explant while genotypes that showed the lowest values were ‘BAT 304’ and ‘BAT 93’. The effect of the variety in this species was shown during the tissues culture process.
Authors and Affiliations
ourdes R. García , Idalmis Bermúdez-Caraballoso , Novisel Veitía 1, Raúl Collado , Damaris Torres , Carlos Rom ero , Amanda Martirena
Keywords
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