A COMPARATIVE EVALUATION OF ENZYMATIC ANTIOXIDANT LEVELS IN PRE AND POST THERAPY PATIENTS WITH ORAL CANCER
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objectives: The present study was aimed to evaluate the magnitude of oxidative stress and levels of enzymatic antioxidants in Oral Squamous cell carcinoma (OSCC) patients receiving radiotherapy (RT) and chemo radiotherapy (CRT). Venous blood samples were collected from 20 healthy subjects, 20 disease control patients (without treatment), 20 oral cancer patients who received chemo radiotherapy and 20 oral cancer patients receiving radiotherapy and were analyzed for antioxidant status using various assay techniques.Methods: The present study measured the levels of three antioxidants enzymes: Superoxide dismutase (SOD), Glutathione Peroxidase (GPX), Catalase (CAT) in the plasma samples of 20 patients who were proven with biopsy-Squamous cell carcinoma of the oral cancer with clinical stage III/IV and were receiving radio and chemo radiotherapy. Same enzymes were also estimated in 20 healthy individuals and disease control patients (who were admitted in to the cancer clinic).Results: The plasma levels of antioxidant enzymes (SOD, CAT, and GPX) were lower in the oral cancer patients as compared to those in the healthy individuals. Superoxide dismutase levels in healthy control patients were found to be 190.4µg/dl, 34.54 µg/dl in disease control patients, 46.16 µg/dl in radiotherapy received group patients and 81.48 µg/dl in chemo and radiotherapy received group patients. Glutathione peroxidase levels were found to be 65.713 µg/dl in healthy control group patients, 13.8 µg/dl in disease control patients, 16.49 µg/dl in radiotherapy received group patients, 34.2 µg/dl in chemo and radiotherapy received group patients. Catalase enzyme levels were found to be 52.37 µg/dl in healthy control group patients, 12.35 µg/dl in disease control group patients, 22.34 µg/dl in radiotherapy received group patients, 27.18 µg/dl inchemo and radiotherapy received group patients. These enzymes also showed significant changes with radiotherapy and chemoradiotherapy. Antioxidant enzymes in the plasma of the oral cancer patients after radiation therapy lowered as compared to the plasma levels of enzymes after chemoradiotherapy.Conclusion: This present study also showed decreased levels of antioxidant enzymes in the plasma of the oral cavity cancer patients after radiation therapy as compared to the chemoradiotherapy receiving oral cancer patients. An appreciable progress in antioxidant levels were observed in patients after receiving chemoradiotherapy (CRT) and observed to be more effective than after radiotherapy (RT). The reason for this observation was believed that concomitant chemoradiation and radiotherapy caused a reduction in the lipid peroxidation process and an improvement in the antioxidant levels of the oral cancer patients. But the radiation therapy produces high oxidative stress when compared to chemoradiotherapy in Oral Squamous Cell Carcinoma.Â
Authors and Affiliations
Sangram Vurumadla, Krishna Kanth Akula
Keywords
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