A COMPARATIVE EVALUATION OF PETROFF’S METHOD AND NALC-NaOH DIGESTION DECONTAMINATION PROCEDURE FOR THE ISOLATION OF MYCOBACTERIA FROM CLINICAL SPECIMENS IN A TERTIARY CARE HOSPITAL
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 69
Abstract
BACKGROUND Tuberculosis is the leading cause of death from a curable infectious disease.[1] Examination by mycobacterial culture provides the only definitive diagnosis of tuberculosis.[3] Isolation of Mycobacteria from specimens contaminated with normal flora like sputum poses a problem in view of high nutritive value of the egg media used for mycobacterial culture. Different laboratories use different decontamination techniques. No single technique is entirely satisfactory. Hence, this study was conducted to compare the Petroff’s method and NALC-NAOH (N-Acetyl-L-cysteine NaOH) method digestion decontamination procedures. MATERIALS AND METHODS This prospective study was conducted over a period of one year (1st August 2009 to 31st July 2010) in the Department of Microbiology, Dayanand Medical College and Hospital, Ludhiana. Samples received from various wards and intensive care units (ICUs) were processed for mycobacterial culture on 2 sets of Lowenstein-Jensen media using two decontamination proceduresPetroff’s method and NALC-NaOH method.[4] The isolation rate and contamination rate of growth on both the sets of LowensteinJensen media was compared. RESULTS The contamination rate of LJ media with Petroff’s method was 20.31%, while the contamination rate with NALC-NaOH method was 14.06%. In our study, the rate of isolation was similar in both the decontamination procedures. The rate of isolation was 10.93% on both the sets of LJ media. CONCLUSION We observed that there was no significant difference in the isolation rate of mycobacteria with the use of either Petroff’s method or NALC-NaOH method, while the contamination rate was higher with Petroff’s method.
Authors and Affiliations
Kawal Preet Kaur, Harender Simar, Pardaman Singh Setia, Bharti Arora, Garima Mehta, Priyanka Meel
Keywords
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- EP ID EP233238
- DOI 10.14260/Jemds/2017/1066
- Views 85
- Downloads 0
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