Abstract

Background: Peptic ulcer disease is a most common disease, whose incidence is increasing presently on account of demanding stressful life. Objectives: To evaluate and compare the antiulcer activity of the extracts of seed and skin of Vitis Vinifera (Grape) using two different doses (100 & 200 mg/kg). Methods: The extracts of seed and skin of Vitis Vinifera were investigated for their anti-ulcer activity using two doses (100 & 200 mg/kg) against aspirin plus pylorus ligation induced gastric ulcer in rats. Wistar albino rats (180 - 250grams) of either sex were divided into 6 groups, with 6 rats per group. All drugs were given orally. All received 200mg/kg Aspirin for 3 days along with Distilled water / Ranitidine / Test compound (water, food – ad libitum). On the 4th day, pylorus was ligated under light ether anaesthesia (after 36 hours fasting). 4 hours later, rats were sacrificed by decapitation. The stomach was opened. Contents were collected and titrated against 0.01N NaOH(Sodium hydroxide) for free acidity and total acidity using indicators – Topfer’s reagent and Phenolphthalein. Ulcers were examined under magnification. Results: A significant reduction (p <0.001) was observed in ulcers, gastric volume, free and total acidity in all groups as compared to the control group. Grape seed 200 mg/kg showed maximum effect in all parameters. Conclusion: Our study indicates that the extracts of seed and skin of Vitis Vinifera has potential anti-ulcer activity in aspirin plus pylorus ligation model, with grape seed 200mg/kg having the best activity

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