A COMPARATIVE STUDY OF DIAGNOSTIC TECHNIQUES FOR MYCOBACTERIUM TUBERCULOSIS INFECTION IN PORT HARCOURT, RIVERS STATE
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2019, Vol 6, Issue 7
Abstract
The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The aim of this study was to compare some diagnostic techniques for Mycobacterium tuberculosis infection in Port Harcourt, Rivers State. A total of one hundred and eighty-two (182) sputum samples were collected from patients including children, teenagers and adults suspected to be infected with tuberculosis (TB) who were visiting the University of Port Harcourt Teaching Hospital, Choba. The samples were transported in cold chain with triple packaging system to be processed in a biosafety level two with level three practices laboratory in South-South Tuberculosis Zonal Reference Laboratory in Port Harcourt using Acid Fast Bacilli microscopy, Rapid Diagnostic Technique (RDT) and GeneXpert MTB/RIF assay. Whole Blood samples were also collected from participants for RDT. The present study showed a prevalence of 31.5% MTB positive cases among the studied population using AFB or GeneXpert. Positive MTB with rifampicin resistance was 65% while rifampicin sensitive was 35%. AFB microscopy of 20.3% showed scanty MTB positive (+) while 10.1% were moderately MTB (++) positive and, 1.1% of the tested population showed a heavy MTB (+++) infection. The positive predictive value of Ziehl-Neelsen microscopy from this study stood at 100% as against 8.0% for RDT. The results showed higher TB prevalence in males than in females. Conclusively, GeneXpert MTB/RIF assay is highly sensitive and specific in the detection of MTB and Rifampicin Resistance. The short turnaround time enhance prompt patient management and controls the transmission of the disease. It is therefore recommended that the MTB/RIF assay be emphasized as point of care test over RDT and AFB microscopy.
Authors and Affiliations
Ibiju Wariboko
Keywords
Related Articles
- EP ID EP671019
- DOI -
- Views 171
- Downloads 0
IMPACT OF ANALYTICAL ERROR IN AN ESTIMATION OF LIVER ENZYMES
Backgrounds: Laboratory professionals should produce accurate, sensitive and specific information using new age technologies to guide clinical decision making. It is the role of laboratory professionals to inform physici...
MARFAN SYNDROME WITH PLEURAL EFFUSION
Introduction: Marfan syndrome is rare autosomal disorder of the connective tissue affect both male and female equally, manifested with skeletal, ligamentous, orooculofacial, ophthalmic, pulmonary, neurological and most f...
SERUM TESTOSTERONE LEVEL IN TYPE 2 DIABETES MELLITUS MALES: A REVIEW IN URBAN POPULATION OF PAKISTAN.
Background: High prevalence of low testosterone levels is evocative due to many chronic conditions, amongst which Diabetes Mellitus is at the top most. Creating such association decisively might contain concentrated resu...
EFFECTS OF MYRISTICA FRAGRANS (NUTMEG) ON COGNITION AND NICOTINE-INDUCED ANTEROGRADE AMNESIA IN WISTAR RATS
Learning, memory and other cognitive functions are essential for the determination and adaptation of responses. Anterograde amnesia resulting from the disruption of cellular activities, if unchecked may accumulate and ha...
NEW DELHI METALLO-Β-LACTAMASE 1 (NDM-1) PRODUCING ESCHERICHIA COLI IN BASRAH HOSPITALS. IRAQ
Resistance to carbapenems is developing around the world Escherichia coli(E.coli) resistance to carbapenems is associated with production of carbapenem-hydrolyzing class B metallo-β-lactamases (MBL).New Delhi metallo-β-l...