A Comparative Study of Three Methods of Hiv Determination in Barau Dikko Specialist Hospital Kaduna, Kaduna State, Nigeria
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 1
Abstract
The need for accurate diagnosis of HIV infection has become a major global issue of interest over the years. This study sets out to determine the sensitivity and specificity of HIV test kits used in Barau Dikko Specialist Hospital Kaduna, Kaduna State, Nigeria. The kits assessed were Determine, Stat-Pak and Uni-Gold HIV1/2 test kits. A total of 200 gold standard positive and 200 gold standard negative sera by ELISA method were used for this study. Of the 200 HIV positive (gold standard) sera tested against each of the kits, 196 were truly positive and 4 were falsely negative using Determine HIV ½ giving sensitivity of 98%, 198 were truly positive and 2 were falsely negative with Stat-Pak, giving a sensitivity of 99% and 197 were truly positive and 3 were falsely negative with Uni-Gold giving a sensitivity of 98.9%. Of the 200 negative sera (gold negative), 195 were truly negative and 5 were falsely positive for Determine giving a specificity of 97.5%. 197 were truly negative and 3 were falsely positive for Stat-Pak giving a specificity of 98.5% while 196 were truly negative and 4 were falsely positive for Uni-Gold giving a specificity of 98%. In all, Stat-Pak scored highest, followed by Uni-Gold and lastly Determine, but the differences were statistically insignificant (p>0.05). The use of these kits for screening blood donors should be discouraged and ELISA method introduced for all blood transfusion purposes as none of the kits exhibited 100% sensitivity and specificity. Stat-Pak should be used as a tier-breaker for screening tests in view of its higher sensitivity and specificity. Assessment of HIV test kits should be an ongoing exercise in all facilities and all test kits newly purchased should be assessed before use.
Authors and Affiliations
Habakkuk Joseph, Ayuba Sunday Buru, J. C Ihongbe
Keywords
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- EP ID EP590725
- DOI 10.9790/0853-1601099599.
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