A COMPARATIVE STUDY ON ONTOGENIC EXPRESSION OF ANTIOXIDANTS AND SECONDARY METABOLITES IN WITHANIA SOMNIFERA
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 1
Abstract
Withania somnifera (WS), also known as Ashwagandha, Indian ginseng and winter cherry, has been an important herb in the ayruvedic and indigenous medical system for over 3000 years. Antioxidant activity and phytochemical analysis of both in vitro and in vivo grown leaves were studied in W. somnifera at different time period. Antioxidants are an important group of medicinal preventive compounds as well as being food additives inhibiting detrimental changes of easily oxidizable nutrients. The estimated antioxidant enzymes were superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase. The analyses were carried out in the leaves of in vitro and in vivo plants. It was found that in vitro leaves contained a significant quantity of enzymatic antioxidants than in the leaves of in vivo plants. Plants are able to produce and accumulate many medicinally valuable secondary metabolites. Search for the concerned active compounds has led to the identification of several withanolides, alkaloids, steroids, phenolics, tannins, saponins and quinones from leaves of W. somnifera. The fresh leaves were extracted with increasing and decreasing polarity of solvents and it was found that ethyl acetate extract contains all the metabolites present in the leaves, analyzed by thin layer chromatography using chloroform: methanol in the ratio of 9:1 as solvent system. The chromatogram was developed using 10% sulphuric acid followed by heating and the spots were visualized. These findings have high significance in the pharmacological industry; however, a detailed investigation is needed to confirm this conclusion, which is underway in our laboratory.
Authors and Affiliations
Nathiya S, Santhi N, Kalaiselvi Senthil
Keywords
Related Articles
- EP ID EP124802
- DOI -
- Views 90
- Downloads 0
STUDY OF EFFECT OF BHASTRIKA PRANAYAMA ON PULMONARY FUNCTION
Yogic practices can be used as psycho-physiological stimuli to increase in physiological functions and human performance. Yoga breathing or Pranayama is the science of breath control. Rapid succession of forcible expulsi...
RUJAKARA MARMA: A CONCEPTUAL REVIEW
Marmas are part of a greater sacred physiology which maps the special sacred points just as the earth has its sacred sites and energy currents according to sacred geography. It is must to learn the sacred geography of ou...
FORMULATION DEVELOPMENT OF ORO-DISPERSIBLE TABLETS OF AYURVEDIC POWDER FORMULATION FOR EFFICIENT USE
The aim of this study was to formulate mouth dissolving tablets from Ayurvedic polyherbal powder Sitopaladi. Sitopaladi is a traditional Ayurvedic powder preparation well known and effective in relieving coughs associate...
SECOND ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TELMISARTAN AND METOPROLOL IN TABLET DOSAGE FORM
Accurate, precise, rapid and economical method was developed for the estimation of Telmisartan (TELM) and Metoprolol (METO) in bulk and tablet dosage form using second order derivative spectrophotometry. Wavelengths sele...
FORMULATION, OPTIMIZATION AND EVALUATION OF STOMACH SPECIFIC IN-SITU GEL OF HYDROCHLOROTHIAZIDE
The objectives of this research work were preparation, optimization and evaluation of in situ gelling system of Hydrochlorothiazide based on pectin that retains in the stomach by adherence to gastric wall providing an in...