A Cross Sectional Study on Prevalence of Antibiotic Resistance and Role of Efflux Pumps in Fluoroquinolone Resistance by using Efflux Pump Inhibitors in Isolated Cultures from Poultry, Dairy Farms and MTCC Strains from Reservoirs
Journal Title: Microbiology Research Journal International - Year 2015, Vol 5, Issue 2
Abstract
Aims: Emergence of antibiotic resistance in bacterial strains has always remained a crucial concern. Mutations in antibiotic target sites, over expression of efflux pump are the major modes of development of bacterial antibiotic resistances. The present study was conducted to determine antibiotic resistance and role of efflux pumps in fluoroquinolone resistance by using efflux pump inhibitors. Place and Duration of Study: The Research was conducted during June 2011 to March 2012 at Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana – India. Methodology: Out of 57 bacterial strains 19 were procured from collection centres (reservoirs) and 38 were isolated from dairy (n = 10) and poultry farms (n = 28) and screened against 12 antibiotics of different groups by well assay. Further, fluoroquinolone sensitive/resistant strains were tested to observe the decline in minimum inhibitory concentration (MIC) levels in the presence/absence of efflux pump inhibitors. Results: Antibiotic resistance in tested strains was higher against nitrofurantoin, lincomycin, cefixime and chloramphenicol. Majority of the bacterial strains (94.74%) showed resistance to two or more antibiotics. Isolated bacterial strains were exhibiting more antibiotic resistance than reservoir strains indicating their exposure to antibiotics. Reduction in MIC (2-4 folds) was observed when Piperine (28.07%) or Plumbagin (19.29%) was used in combination with fluoroquinolones. The findings emphasized that majority of efflux pump inhibitors are active against Gram-positive bacteria. Overexpression of efflux pump and higher antibiotic resistance was also observed in subgroup III exhibiting resistance to combination of cefixime/nitrofurantoin. Conclusion: Efflux mediated resistance appears to contribute significantly to fluoroquinolone resistance and multidrug resistance in organisms, which may be due to involvement of active efflux pumps of both MFS and RND Family.
Authors and Affiliations
Leena Seasotiya, Priyanka Siwach, Pooja Bharti, Sheema Bai, Anupma Malik, Ravinder Kumar, Sunita Dalal
Keywords
Related Articles
- EP ID EP354542
- DOI 10.9734/BMRJ/2015/12342
- Views 83
- Downloads 0
Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)
Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses and celluloses as important components of plant cell wall. This enzyme is essential in wide range of applications...
Corneal Ulcers at the University of Calabar Teaching Hospital in Nigeria - A Ten Year Review
Aim: To review the pattern, trend, predisposing factors, characteristics and visual outcome of corneal ulcers at the University of Calabar Teaching Hospital (UCTH) between January 2003 and December 2012 with a view to pr...
Used Toothbrushes: Microbial Evaluation and Antibiotic Susceptibility Profiles of Associated Bacteria
Aims: Toothbrush has been universally accepted as the most effective tool for removing harmful plaque and bacteria from mouth. On average, colonization of bacteria is reduced by 88.8% as a result of brushing. Toothbrush...
Food-borne Bacteria: Occurrences, Multidrug Resistant Patterns and Susceptibility to Aqueous Leaf Extracts of Acalypha hispida (Linn)
The occurrence and susceptibility of multidrug resistant (MDR) bacteria obtained from food to aqueous leaf extracts of Acalypha hispida (ALEAH) were determined using standard bacteriological and disc diffusion methods. I...
Changes in Serum Levels of TNF-α & IL-4 among New, Under-treatment & MDR TB Patients
Introduction: Tuberculosis (TB) remains a significant public health problem with an estimated one-third of the world’s population being infected. Cytokines play a major role in protection against Mycobacterium tuberculos...