A Fluorescence Microscopy Study of Seminal Fluid In Infertile Males Using Acridineorange Dye To Assess DNA Integrity Along With Semen Analysis
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 10
Abstract
According to World Health Organization(2000), infertility is the inability of the sexually unprotected, active couple to achieve pregnancy in one year of marriage . Present study is aimed to assess DNA damage in sperms using Acridine Orange fluorescence technique in infertile malesto correlate the results of DNA damage with other semen parameters and to select the healthy semen donors in sperm banks as part of treatment of infertility. Collaborating the findings from our study and the previous studies in the literature, it is concluded that fluorescence microscopy of seminal fluid using acridine orange is an easy test to perform and a relatively cheap method as compared to other methods which are expensive and require more machinery, equipment’s and skilled technical staff. Although easy to perform and relatively cheap, it does not loses its relevance in semen analysis in comparison to other tests.
Authors and Affiliations
Dr. Gaurav Pawar, Dr. Kuldip Kumar, Dr. Chanpreetkaur pawar, Dr. Manoj K. Sharma, Dr. Jaspinderpratap Singh, Dr. Harmohinder K Attri
Keywords
Related Articles
- EP ID EP401593
- DOI 10.9790/0853-1710032532.
- Views 88
- Downloads 0
Caffey Disease or Infantile Cortical Hyperostosis: A Case Report
Infantile cortical hyperosteosis, also known as caffey`s disease,is a rare, idiopathic mostly self limiting condition affecting infants under 6 months of age. It is characterized by acute inflammation of the periostium a...
An Evaluation of The Clinical And Etiological Profile of Acute Renal Failure in Obstetric Patients: An Observational CrossSectional Study
Objective: To evaluate the clinical and etiological profile of acute renal failure (ARF) in obstetric patients in Bundelkhand region. Methods: This was observational cross-sectional study. The women with previously healt...
Prevalence of Cutaneous Changes in Chronic Renal Failure Patient On Haemodialysis in Tertiary Care Center of Jharkhand.
Abstract:Chronic renal failure (CRF) presents with an array of cutaneous manifestations. Cutaneous signs of renal failure are mainly related to chronicity of disease. We evaluated the prevalence of various dermatological...
“A Comparative Study Between Biers’ Block And Supraclavicular Brachial Plexus Blockfor Upper Limb Below Elbow Surgeries”
Background And Objectives: Regional anaesthesia is becoming more popular especially with the advent of safer drugs and techniques; This study was conducted in elective surgeries of upper limb below the elbow to compare t...
Laparoscopic Cholecystectomy Under Low Thoracic Combined Spinal Epidural Anaesthesia: A Comparative Study Between Isobaric And Hyperbaric Bupivacaine
Background: Thoracic spinal anaesthesia has proven its efficacy over general anaesthesia as a routine anaesthetic technique for laparoscopic surgeries. This study aimed to compare the block characteristics of isobaric an...