A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes
Journal Title: Polish Journal of Microbiology - Year 2018, Vol 67, Issue 1
Abstract
Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However, information on the enrichment and production of ureolytic microbes by using bioreactor systems is limited. Here, we report a low-tech down-flow hanging sponge (DHS) bioreactor system for the enrichment and production of ureolytic microbes. Using this bioreactor system and a yeast extract-based medium containing 0.17 M urea, ureolytic microbes with high potential urease activity (> 10 μmol urea hydrolyzed per min per ml of enrichment culture) were repeatedly enriched under non-sterile conditions. In addition, the ureolytic enrichment obtained in this study showed in vitro calcium carbonate precipitation. Fluorescence in situ hybridization analysis showed the existence of bacteria of the phylum Firmicutes in the bioreactor system. Our data demonstrate that this DHS bioreactor system is a useful system for the enrichment and production of ureolytic microbes for MICP applications.
Authors and Affiliations
Masataka Aoki, Takuya Noma, Hiroshi Yonemitsu, Nobuo Araki, Takashi Yamaguchi, Kazuyuki Hayashi
Keywords
Related Articles
- EP ID EP270367
- DOI 10.5604/01.3001.0011.6144
- Views 94
- Downloads 0
<strong>Evaluation of the Time Period for Which Real-Time Polymerase Chain Reaction Detects Dead Bacteria</strong>
Real-time polymerase chain reaction (PCR) is currently used widely for the diagnosis of infectious diseases. We evaluated the time required for which real-time PCR can detect bacteria that had been killed [i]in vitro[/i]...
Enzymes Involved in Naproxen Degradation by Planococcus sp. S5
Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its deg...
Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulat...
Application of Metagenomic Analyses in Dentistry as a Novel Strategy Enabling Complex Insight into Microbial Diversity of the Oral Cavity
The composition of the oral microbiome in healthy individuals is complex and dynamic, and depends on many factors, such as anatomical location in the oral cavity, diet, oral hygiene habits or host immune responses. It is...
Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approaches
Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investi...