A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE), A VALUABLE MEDICINAL PLANT
Journal Title: FLORA AND FAUNA - Year 2018, Vol 24, Issue 1
Abstract
An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.
Authors and Affiliations
SUSANTA KUMAR MAITY
Keywords
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- EP ID EP561507
- DOI 10.33451/florafauna.v24i1pp16-20
- Views 115
- Downloads 0
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