A Modified Genomic DNA Extraction Method from Leaves of Sunflower for PCR Based Analyzes
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 6
Abstract
Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small amounts of plant leaves and good quality of sunflower genomic DNA can be isolated. Quantity and purity of extracted DNA were checked by spectrophotometry and agarose gel electrophoresis. This modified method takes less time, reliable and gives good quality of DNA even without using RNase can be used in various marker systems such as (Single Sequence Repeat) SSR and (Random Amplified Polymorphic DNA) RAPD.
Authors and Affiliations
Hossein Zeinalzadehtabrizi, Arash Hosseinpour, Murat Aydin, Kamil Haliloglu
Keywords
Related Articles
- EP ID EP38695
- DOI -
- Views 190
- Downloads 0
Physiological and biochemical characterization of Cork Oak Seedlings (Quercus suber.L) in Tunisia
The cork oak is native forest species, the most represented in Tunisia and especially to Mogods Kroumirie. This species is considerable regression estimated at 1.22% of the area per year . Natural regeneration of cork oa...
Assessment of Variance Indices of Araneid Fauna Grasped from Sugarcane Field by different trapping Methods at Faisalabad, Pakistan
Spiders play integral ecological favor for regulating integrated pest management population. Despites, there is restricted research data on the species richness, evenness and relative abundance in Faisalabad, Pakistan .A...
Biodiversity of lebak deling swamp in pampangan, ogan komering ilir regency, south sumatera province, Indonesia
Investigation on biodiversity of Lebak Deling swamp in Pampangan, Ogan Komering Ilir Regency, South Sumatra Province was carried out from March to September 2011, focused on evaluating the diversity of benthic organism...
Isolation and Identification of Keratin Degrading (keratinolytic) Bacteria from Poultry Feather Dumping Sites
Poultry industries generate a large quantity of feather waste. These feathers contain high quantity of keratin which can be bio-converted into peptides and amino acids using bacteria to make value added products. Therefo...
Effect of irrigation period on some morphological traits of Guar (Cyamopsis Tetragonoloba) in karaj region
In order to evaluate irrigation period on some morphological traits of guar plant, a randomized complete block design experiment with three replications was conducted in 2015 and 2016. Irrigation treatments included thre...