A network pharmacology approach to investigate the mechanism of “Huangqi-Shanzhuyu” in the treatment of diabetic nephropathy
Journal Title: Precision Medicine Research - Year 2020, Vol 2, Issue 2
Abstract
Background: To explore the action mechanism of Huangqi (Radix Astragali) and Shanzhuyu (Fructus Corni) in the treatment of diabetic nephropathy based on network pharmacology, aiming to provide a basis for clinical application. Methods: The main active components of Huangqi and Shanzhuyu were discerned through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Traditional Chinese Medicine Integrated Database (TCMID). The targets related to diabetic nephropathy (DN) were obtained using Genecards, Therapeutic Target Database (TTD), and National Center for Biotechnology Information (NCBI) Gene. Subsequently, protein-protein interaction (PPI) networks were constructed with Cytoscape 3.7.2 and the STRING database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to analyze the intersection of targets via Database for Annotation, Visualization, and Integrated Discovery (DAVID) 6.8. Built on the above analysis, we made a “Chinese medicine-chemical composition-target gene-disease” network. Results: Twenty-one active components were predicted from the Huangqi and Shanzhuyu herb pair, such as jaranol, mandenol and sitosterol. These components were applied to 41 targets mainly involved in many biological processes such as the PI3K-Akt signaling pathway, vascular endothelial growth factor (VEGF) signaling pathway, regulation of sodium ion transport and steroid-binding. Conclusion: This study proposes the network pharmacology method and identifies the potent combination therapeutic mechanism of Huangqi and Shanzhuyu for diabetic nephropathy (DN) through multiple targets and routes, this strategy will lay a good foundation for further in-depth study of the mechanism of action.
Authors and Affiliations
Hao-Yu Yu , Yu-Liang Zhang , Qian Cui , Guo-Wei Zhang
Keywords
Related Articles
- EP ID EP685211
- DOI 10.12032/PMR201900028
- Views 187
- Downloads 0
Use of network pharmacology and molecular docking to explore the potential mechanism governing the efficacy of Jinhuaqinggan granules in the treatment of novel coronavirus-induced pneumonia
Background: Now that the epidemic of new coronavirus pneumonia (corona virus disease 2019) is spreading all over the world, Jinhuaqinggan granules in the Chinese treatment plan has been proved to be an effective Chinese...
A network pharmacology approach to investigate the mechanism of “Huangqi-Shanzhuyu” in the treatment of diabetic nephropathy
Background: To explore the action mechanism of Huangqi (Radix Astragali) and Shanzhuyu (Fructus Corni) in the treatment of diabetic nephropathy based on network pharmacology, aiming to provide a basis for clinical appli...
Screening key target genes for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on bioinformatics and gene network
Background: To provide a reference for the clinical development of drugs to suppress severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Retrieving genes related to SARS-CoV-2 with Genecards database...
Screening key target genes for pulmonary arterial hypertension based on bioinformatics
Objective: Screening key target genes for pulmonary arterial hypertension (PAH) based on bioinformatics to provide a reference for the clinical development of drugs to cure PAH. Methods: The keyword “pulmonary arterial h...
Network pharmacological approach to explore the mechanisms of Lianhua Qingwen capsule in coronavirus disease 2019
Background: This study aimed to explore the molecular mechanisms of the active compounds of Lianhua Qingwen capsule in the treatment of coronavirus disease 2019 by using systemic pharmacology approach. Methods: In this...