A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2019, Vol 9, Issue 6
Abstract
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug.
Authors and Affiliations
Islamudin Ahmad, Neneng Siti Silfi Ambarwati, Berna Elya, Hanita Omar, Kamarza Mulia, Arry Yanuar, Osamu Negishi, Abdul Mun'im
Aqueous extracts of microalgae exhibit antioxidant and anticancer activities
Objective To investigate the antioxidant and anticancer activities of aqueous extracts of nine microalgal species. Methods Variable percentages of major secondary metabolites (total phenolic content, terpenoids and al...
Proximate composition, nutritional values and phytochemical screening of Piper retrofractum vahl. fruits
Objective: To investigate the proximate and mineral composition of the Piper retrofractum (P. retrofractum) vahl. Fruit and to evaluate its total alkaloids, phenol and flavonoid. Methods: The proximate composition of P....
The synergistic effect of honey and cinnamon against Streptococcus mutans bacteria
Objective: To investigate the effect of Iranian honey, cinnamon and their combination against Streptococcus mutans bacteria. Methods: Nine experimental solutions were examined in this study, including two types of honey...
Generation of antibodies against disintegrin and cysteine-rich domains by DNA immunization: An approach to neutralize snake venom-induced haemorrhage
Objective: To explore whether a DNA immunization approach targeting the major haemorrhage molecule of a prothrombin activator-like metalloproteinase from Echis ocellatus (E. ocellatus) venom could be conceived to inspire...
Laboratory efficacy of mycoparasitic fungi (Aspergillus tubingensis and Trichoderma harzianum) against tropical bed bugs (Cimex hemipterus) (Hemiptera: Cimicidae)
Objective: To test the effectiveness of conidial spore formulations [Aspergillus tubingensis (A. tubingensis) and Trichoderma harzianum (T. harzianum)] against tropical bed bugs, Cimex hemipterus. Methods: Spore formulat...