A new look at an old case: An auto-anti-P with pseudo-LKE activity
Journal Title: International Journal of Blood Transfusion and Immunohematology - Year 2013, Vol 3, Issue 1
Abstract
Aims: LKE is a high-incidence, minor RBC glycosphingolipid, related to both Pk and P antigens. Approximately 1% individuals are LKE-negative. However, antibodies against LKE are rare, with only six cases mentioned in literature. Past examples of anti-LKE have relied on serologic testing, with no direct testing against RBC glycosphingolipid (GSL). To test a historical 'anti-LKE' against a panel of RBC and glycosphingolipid standards by high performance thin layer chromatography and standard serology. Methods: Serum samples included human polyclonal anti-LKE, alloanti-P, alloanti-PP1Pk and untransfused controls. Hemagglutination was performed by gel method with ficin-treated RBC of known LKE, P and P1 phenotype. P antigen expression was determined by titration with a well characterized alloanti-P. Antibody specificity was determined by incubating serum against glycosphingolipids on high performance thin layer chromatography plates. Results: The patient's serum reacted with most LKE+ RBC but not ficin-treated p, Pk, or LKE-negative donors, consistent with an anti-LKE. However, on direct testing, the patient's antibody failed to recognize monosialogalactosylgloboside, the LKE antigen. The patient's serum did recognize globoside (P) antigen. This was confirmed by hemagglutination, which showed a correlation between LKE phenotype, P antigen expression and serum reactivity. The patient's weak auto-anti-P was not inhibited by solubilized globoside. Conclusion: This historical anti-LKE is an auto-anti-P with 'pseudo-LKE' activity due to differences in P antigen expression between LKE+ and LKE-donors.
Authors and Affiliations
Laura Cooling
Keywords
Related Articles
- EP ID EP210074
- DOI 10.5348/ijbti-2013-10-OA-1
- Views 73
- Downloads 0
Analysis of the evolution of the residual risk of HIV transmission at the National Blood Transfusion Center (CNTS) of Lomé (Togo) from January 2008 to December 2012
Aims: This study aimed to: - Analyze the HIV residual risk of transfusion at the CNTS of Lomé from January 2008 to December 2012 - Indicate future directions to strengthen blood safety. Methods: The residual risk of HIV...
Pattern of blood donor deferral in a tertiary hospital, South-south, Nigeria: A three-year study review
Aims: Provision of safe and adequate supply of blood and blood products is a major public health issue globally. Safe blood is ensured through proper donor selection and testing of donated blood. However, a large number...
Serosurvey of human T cell lymphotropic virus I/II among blood donors in Gombe (Nigeria)
Aim: This study was carried out to determine the prevalence of human T cell lymphotropic virus I/II (HTLV I/II) among blood donors at the Federal Teaching Hospital Gombe (FTHG), North-eastern, Nigeria. Methods: A total...
A new look at an old case: An auto-anti-P with pseudo-LKE activity
Aims: LKE is a high-incidence, minor RBC glycosphingolipid, related to both Pk and P antigens. Approximately 1% individuals are LKE-negative. However, antibodies against LKE are rare, with only six cases mentioned in lit...
The prevalence of transfusion transmitted infections: A focus on hepatitis B virus among blood donors at Hawassa blood bank center, Southern Ethiopia
Aims: Millions of lives are saved each year through blood transfusion but a safe blood supply is a critical component of health care to prevent the spread of blood-borne infectious diseases. Therefore, it is essential to...