A NEW METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF ELETRIPTAN BY USING RP-HPLC METHOD
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 5
Abstract
A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for estimation of eletriptan in tablet dosage form. Chromatographic separation was carried out by using mobile phase 0.02M potassium dehydrogenate Phosphate buffer (ph 3.0) : Acetonitrile (50:50 v/v, PH-3.0 adjusted with Orthophosphoric acid ) on Hypersil, Symmetry C8 150×4.6mm, 5m at flow rate 0.8ml/min in isocratic mode and effluents are monitored at 221 nm. The retention time for eletriptan was found to be 2.45 min respectively and calibration curves were linear over concentration from 10-50μg/ml. The method was validated for accuracy, precision, specificity, linearity, system suitability. LOD and LOQ were 0.0258μg/ml and 0.0897μg/ml for eletriptan. The percentage recovery of eletriptan was found to be 98-102%.The developed method was fast, accurate, precise, and successfully applied to estimate the amount of eletriptan in bulk sample and tablet dosage form so it can be used for quality control department
Authors and Affiliations
S. Sirish Chandra , T. Priyanka , Naga Sowjanya , A. Ajitha , Uma Maheswara Rao
Keywords
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- EP ID EP106582
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