A new validated HPTLC method for quantitative determination of 1, 5-dicaffeoylquinic acid in Inula crithmoides roots
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2012, Vol 25, Issue 4
Abstract
1, 5-Dicaffeoylquinic acid (1, 5-DCQA), a potent HIV-1 integrase inhibitor, is currently undergoing an evaluation as a promising novel HIV therapeutic agent. This work aims at developing an accurate, rapid, repeatable and robust HPTLC method for the determination of 1, 5-DCQA in its natural sources. 1, 5-DCQA is the major component of the n-butanol fraction, the most biologically active hepatoprotective fraction, of Inula crithmoides roots extract. Thus, it will be of interest to evaluate the plant roots as a potential source of 1, 5-DCQA using a fully validated HPTLC method. The percentage of 1, 5-DCQA in the studied plant (0.035% w/w) was found to be approximately similar to those previously determined in other antioxidant herbal drugs, in which 1, 5- DCQA is the main phenolic constituent. The results obtained showed that the described HPTLC method is suitable for routine use in quality control of herbal raw material, extracts and pharmaceutical preparations containing 1, 5-DCQA. No HPTLC method has been reported in literature for the determination of 1, 5-DCQA in medicinal plants.
Authors and Affiliations
Maha Aboul-Ela, Abdalla El-Lakany, Safa Shams Eldin, Hala Hammoda
Keywords
Related Articles
- EP ID EP156136
- DOI -
- Views 68
- Downloads 0
Annona squamosa Linn: Cytotoxic activity found in leaf extract against human tumor cell lines
Cancer is a common cause of death in human populations. Surgery, chemotherapy and radiotherapy still remain the corner stone of treatment. However, herbal medicines are gaining popularity on account of their lesser...
Synthesis and antibacterial screening of S-substituted derivatives of 5-(3,4-methylenedioxyphenyl)-1,3,4-oxadiazol-2-thiol
The most emerging class among the heterocyclic compounds is 1,3,4-oxadiazoles for their diverse biological activities. In the present research work, piperonylic acid (1) was converted consecutively into correspondi...
Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line
Crude hydromethanolic (80 percent methanol) extracts produced by maceration of Onopordum acanthium leaves and Spartium junceum flowers were tested for cytotoxic effects against glioblastoma U-373 tumour cells. Onop...
Mining of protein based biomarkers for type 2 diabetes mellitus
Diabetes mellitus in general and Type 2 Diabetes (T2D) in particular, are very complex diseases with heterogeneous etiology. T2D results from the impaired secretion or action of the insulin with a clinical phenotype of p...
In vitro synergistic effect of ciprofloxacin with aminoglycosides against multidrug resistant-Pseudomonas aeruginosa
Pseudomonas aeruginosa is an increasingly prevalent nosocomial human pathogen. Infections with multidrug-resistant (MDR) P. aeruginosa are currently a treatment challenge and requires search for better treatment op...