A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs
Journal Title: Animal Science Papers and Reports - Year 2012, Vol 30, Issue 2
Abstract
The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 537 amino acids, which is homologous with the Copine I (CPNE1) of eight species: greater horseshoe bat (95%), mouse (92% ), rat (93%), cattle (95%), sheep (95%), rabbit (94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the swine CPNE1 gene and had been assigned GeneID: 100233174. The phylogenetic analysis revealed that the swine CPNE1 gene has a closer genetic relationship with the CPNE1 gene of a dog. The tissue expression analysis indicated that the swine CPNE1 gene has a broad tissue distribution. The experiment described is the first to establish the primary foundation for further research on the swine CPNE1 gene.
Authors and Affiliations
Pu Shifei, Kong Chuisi, Liu Yonggang
Keywords
Related Articles
- EP ID EP70750
- DOI -
- Views 121
- Downloads 0
Concentration of harmful gases in poultry and pig houses
A common chicken broilers rearing house and fattening pig barn were monitored for harmful gases concentration (NH3, CO2, NO2 and CH4). In chickens the concentrations of NH3 rose during fattening periods in all seasons of...
Effect of wheat gluten and extracted soybean meal added to the diet of cows with different kappa-casein genotypes on the composition and physical properties of milk
The effect of adding wheat gluten or extracted soybean meal (300 g of crude protein/head/day) on milk yield, composition and physical properties, was analysed on 53 Polish Holstein-Friesian cows with different milk kappa...
Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation
Fifteen early-lactation cows and 15 mid-lactation cows were chosen for the analysis. Blood samples were collected to measure the beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), glucose...
Bovine mammary stem cells studies – current status – a review
Bovine mammary gland is a unique organ with regard to its frequently repeating cycles of growth and involution throughout the life of an animal. In literature there is only scarce information concerning development of it...
Associations of new rSNPs with eggshell thickness in Rhode Island layers
Six fragments were sequenced of the regulatory and coding regions of the expressed sequence ChEST985k21 (accession number CR523443), which had been shown to affect eggshell thickness. Six sites of single nucleotide polym...