A Novel RP-HPLC Method for Simultaneous Determination of Moxifloxacin and Atorvastatin
Journal Title: RADS Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 5, Issue 4
Abstract
Objectives:A RP-HPLC method was validated which is simple, rapid and accurate for simultaneous determination and quantification of Moxifloxacin in presence of Atorvastatin. Methodology:Chromatographic separation was achieved using C18 having column size of 250x4.6mm, 5µm column. The mobile phase used was composed of methanol: water (95:5v/v) pumped at 1.0mL min-1 flow rate. The detection was performed at 250nm. Result:The results presented complete separation of peaks with good resolution for both active ingredients in the analyte. The values of intra and inter-day precision were also found to be within required limits. The LOD and LOQ were estimated. Validation process also showed stability of both the drugs at altered pH, wavelength, flow rate and concentration of mobile phase also seemed to unaffect the results. The method was validated and all the parameters of method validation were found to be in good agreement. Conclusion: Overall, the method revealed acceptable linearity and good correlation for both the drugs. The method possessed high precision value, accuracy and % recoveries of both drugs.The developed method can therefore be a valuable tool for regular simultaneous analysis of both drugs in API and bulk dosage form.
Authors and Affiliations
Fatima Qamar
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