A PHYSICO-CHEMICAL CHARACTERIZATION OF A NATURAL AGGLUTININ FROM THE MUCUS OF A SLUG LAEVICAULIS ALTE (FERUSSAC, 1822)
Journal Title: International Journal of Zoology and Research (IJZR) - Year 2018, Vol 8, Issue 3
Abstract
Natural hemagglutinin, which is highly specific for the glycocalyx of rabbit erythrocytes is identified in the gastropodanmollusc Laevicaulisalte. Of the tissues/fluids of the said species tested for hemagglutination activity, maximum hemagglutin ability is observed in the mucus. Physico–chemical characterization of the mucus agglutinin of L. alte shows optimum agglutinability at pH 7.5 and temperature 55° C. Agglutinability is altered by the inclusion of diverse concentrations of cations or chelators in the buffer. The agglutinability is lost fully, when the mucus is pre-adsorbed with any one erythrocyte that is recognized by the agglutinin. The hemagglutinability of the agglutinin with rabbit erythrocytes is inhibited by the glycoproteins fetuin, α acid glycoprotein, bovine submaxillary mucin, lactoferrin, apotransferrin and the sugars maltose, N-acetyl neuraminic acid, lactose and galactose. Decline in hemagglutination activity when mixed with the asialo rabbit erythrocytes and reduction in hemagglutination inhibition titer when treated with desialylated fetuin, α acid glycoprotein, bovine submaxillary mucin, and lactoferrin reveals the affinity of L. alte mucus agglutinin to sialic acid
Authors and Affiliations
SR MR BASIL-ROSE A. PUNITHA, ARUL GANDHI A. , VISWAMBARI DEVI R, JOSEPHINE PRIYATHARSHINI C
Keywords
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- EP ID EP430383
- DOI 10.24247/ijzroct20182
- Views 100
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