A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics
Journal Title: Journal of Clinical and Medical Research - Year 2020, Vol 2, Issue 2
Abstract
Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.
Authors and Affiliations
Ingridi de Souza Sene1,2, Vladimir Costa2, Debora Cavalcante Braz2,3, Emanuel Airton de Oliveira Farias4, Gisele Elias Nunes5, Ivan H Bechtold5, Livio Cesar Cunha Nunes3, Carla Eiras6* and Carlos Henrique Nery Costa1,2
Keywords
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- EP ID EP708386
- DOI https://doi.org/10.37191/Mapsci-2582-4333-2(2)-032
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