A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics
Journal Title: Journal of Clinical and Medical Research - Year 2020, Vol 2, Issue 2
Abstract
Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.
Authors and Affiliations
Ingridi de Souza Sene1,2, Vladimir Costa2, Debora Cavalcante Braz2,3, Emanuel Airton de Oliveira Farias4, Gisele Elias Nunes5, Ivan H Bechtold5, Livio Cesar Cunha Nunes3, Carla Eiras6* and Carlos Henrique Nery Costa1,2
Keywords
Related Articles
- EP ID EP708386
- DOI https://doi.org/10.37191/Mapsci-2582-4333-2(2)-032
- Views 58
- Downloads 0
Percutaneous Transluminal Angioplasty In The Treatment Of Diabetic Foot
Background: Diabetic foot is the foot tissue damage, ulceration, secondary segmental necrosis in diabetic patients. The prevalence of diabetes is predominantly due to long-term high blood sugar, high blood lipids in the...
Uterine Endometrial Sarcoma with Carcinomatosis Treated with Iterative Surgery Associated with Systemic and Intraperitoneal Chemotherapy
Introduction: Endometrial sarcoma is a rare condition, affecting women in their 60s. If total hysterectomy associated with bilateral adnexectomy is the standard treatment and can control the disease when it is localized...
Development of Animal Models, Presenting Appropriate Analogues to Respective Human Systems
The main idea of the current study was directed to developed appropriate experimental animal models, imitating respective systems with the human origin, and giving a possibility when the last is not available, experiment...
Changes in Visual Parameters in an Adult Amblyope With Vision Therapy
Amblyopia is a common neurodevelopmental visual disorder that results in reduced contrast, motor perception, and excessive crowding along with impaired visual acuity. Literature suggested this disorder can be treated wit...
Hydatid Cyst of Liver Ruptured in the Portal Vein
Introduction: The rupture of a hydatid liver cyst in the portal system is an exceptional complication. It has been reported only few observations in the world literature. We report a case a patient that we operated on fo...