A Preliminary Phylogenetic Analysis and Comparative Study on the Molecular and Conventional Identities of Bacteria Isolated from Typhoid Fever Patients Attending Some Hospitals in Ondo State, Nigeria
Journal Title: Biotechnology Journal International - Year 2015, Vol 8, Issue 2
Abstract
Aim: This study aimed at determining the molecular and conventional identities of bacteria associated with presumptive typhoid fever patients in Ondo State, Nigeria. Study Design: The study attempted a comparative study of the conventional and molecular identities of bacteria isolated from presumptively diagnosed typhoid patients. Place and Duration of the Study: The study was conducted in three different hospitals in Ondo State, Nigeria viz; State Specialists’ Hospital, Akure, Don Bosco Clinic, Akure and Federal Medical Centre, Owo, between February and May, 2013. Methodology: A total of 520 blood samples were collected from presumptively diagnosed typhoid fever patients attending Don Bosco Clinic Akure, Federal Medical Center, Owo and State Specialists’ Hospital, Akure, Ondo State, Nigeria. The samples were screened for the presence of Salmonella typhi and other co-existing bacteria through blood culture. Initial identification of the isolates was carried using their culture morphology, Gram stain and biochemical tests, while their identities were verified using molecular techniques like PCR analysis, gene sequencing and blasting. The genetic relatedness of the bacterial isolates was investigated by constructing a phylogenetic tree using neighbor-joining (NJ) method. Results: The traditional identities of the bacteria were Salmonella typhi, Escherichia coli, Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae, Enterobacter aerogenes, Serratia marcescens and Pseudomonas aeruginosa while their molecular identities were Enterobacter cloacae, Shigella flexneri, Enterococcus casseliflavus, Achromobacter xylosoxidans, Bacterium species clone BAO 14, Serratia marcescens and Pseudomonas aeruginosa respectively. Most of the isolates fell within the same clade on the phylogenetic tree signifying that they had a common ancestor. Conclusion: Molecular biology techniques are more reliable, sensitive and accurate for bacterial identification than the conventional methods.
Authors and Affiliations
O. E. Ajayi, B. E. Boboye
Keywords
Related Articles
- EP ID EP237233
- DOI 10.9734/BBJ/2015/17275
- Views 101
- Downloads 0
Identification of Suitable Condition for Mannanase Production by Bacillus sp. GA2(1)
Aims: The effects of 1% (w/v) supplementation of additional 5 agricultural wastes, corn cob, bagasses, coffee residues, soybean meal, and copra meal for mannanase production by Bacillus sp.GA2(1) were studied. Hence, par...
Efficient Micropropagation of Spilanthes acmella (L.) Murr.: A Threatened Medicinal Herb
The present study describes an efficient and reproducible protocol for micropropagation of S. acmella. Shoot tips taken from 3 week-old aseptic seedlings were cultured on Murashige and Skoog (MS) semi-solid medium supple...
Processing and Quality of “red kapsiki” an Opaque Beer from “Mandara” Mountain in Cameroon
Aims: Mandara is a chain of Mountains located in northern Cameroun with the border of the republic of Nigeria. This area is populated by “kapsiki” an indigenous populations living. This non moslem population is brewing a...
Cellulase Producing Potential of Aspergillus terreus Uv2 on Cellulosic Wastes Pretreated with Acid and Alkali
Aims: Cellulases offer very wide applications in biotechnology and enzymes from microbial origins present inexpensive source. Production of value added chemicals from wastes will be an exciting translation from waste to...
Genomic Analysis of Fungal Species No.11243 Mutant Strains Provides Insights into the Relationship between Mutations and High Productivity
Aims: FR901469 is a novel antifungal antibiotic produced by fungal species No.11243. Although we have several FR901469 high-producing mutant strains, the mechanism of high productivity is unclear. This study aims to unra...