A PROSPECTIVE STUDY ON THE ISOLATION, CULTURE, SUB-CULTURE AND SPECIES IDENTIFICATION OF CANDIDA FROM THE MUCOSAL LESIONS OF IMMUNO-COMPROMISED INDIVIDUALS
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 14
Abstract
BACKGROUND History of candidiasis dates back to 4th century BC, when Hippocrates described oral aphthae (Thrush) in two patients with severe underlying disease. The initial discovery of thrush organism was made in 1839 by Langenbeck, who observed a fungus in the scrapings of buccal thrush from a patient with typhus. In 1849, Wilkinson first described vaginal candidiasis and its mycotic origin; the dimorphic nature of thrush fungi was noticed by Grawitz in 1877. Fungal infection in human beings ranges from those involving only the most superficial areas of the body and those in which subcutaneous tissue is involved and which sometimes can cause deep and systemic infections that often involved most of the major organs of the body, particularly in an immunocompromised background. Immuno-compromised individuals are those who have a compromised defence system of the body either directly as a result of the disease per se or as a result of treatment with immunosuppressive drugs that the primary ailment they suffer from warrants. This study focuses mainly on the isolation, culture, sub-culture and species identification of candida from the mucosal lesions of immuno-compromised individuals. MATERIALS AND METHODS This descriptive study was conducted at the Government General Hospital, Chennai. One hundred and twenty (120) patients who were either on immunosuppressive therapy or suffering from acquired immunodeficiency states, attending the Department of Dermatology were screened for the present study. Cases were included in the study after application of inclusion and exclusion criteria and after confirming the diagnosis through appropriate investigations including histopathological and serological studies. RESULTS Out of sample size of 120 patients, 100 patients (males- 62, females- 38) were found to be positive for mucosal candidiasis. Following culture in Sabouraud’s Dextrose Agar and sub-culture in the indicator media of HiCrome Candida Agar the following species of candida were isolated, viz. Candida albicans (52%), C. tropicalis (22%), C. glabrata (21%), C. krusei (3%) and C.dubliniensis (2%). CONCLUSION Candidiasis is the commonest mycosis in patients on systemic immunosuppressive therapy and immunosuppressive status like HIV infection. Candida albicans was the commonest species isolated (52%). Acute pseudomembranous glossitis was the commonest morphological type of lesion encountered in this study (35%). Even in HIV infected individuals, Candida albicans was the commonest species isolated in our study.
Authors and Affiliations
Rajkumar Kannan, Janaki Chellam, Kannan Ponnusamy
Keywords
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- EP ID EP414343
- DOI 10.14260/jemds/2018/393
- Views 95
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