A Protocol for Human Serum Fucoxanthinol Quantitation using LC-MS/MS System
Journal Title: Journal of Nutritional Medicine and Diet Care - Year 2016, Vol 2, Issue 2
Abstract
Fucoxanthin, a marine carotenoid widely distributed in brown algae, is known to possess various health promoting activities including anti-obesity effects in humans, but little is known about its kinetics partly because of its low bioavailability. We have developed a simple and reproducible protocol for quantifying system of human serum fucoxanthinol, a fucoxanthin metabolite, used LC-MS/MS multiple reaction monitoring (MRM), and have applied for clinical trial to study fucoxanthin absorption. We established a MRM channel for fucoxanthinol (m/z 617.5 for Q1 and 109.0 for Q3 channel) with a standard curve of R2 > 0.99 and an optimized pretreatment of human serum samples. Fucoxanthinol levels in sera obtained from subjects participating in clinical trials were then measured and a significant increase in the levels was observed at 4 hr after a single oral dose of 22 mg fucoxanthin using newly developed protocol. We propose that use of this protocol could enhance clinical investigation involving the bioavailability and functionality of fucoxanthinol.
A New Whey Cheese Analogue Made from Whey Protein Concentrate and Vegetable Fat with 15% Olive Oil
In this study a whey cheese analogue was made using as basic ingredients whey protein concentrate 65 and vegetable fat with 15% olive oil. The product was enriched with thyme leaves, film packed and then subjected to hea...
A Protocol for Human Serum Fucoxanthinol Quantitation using LC-MS/MS System
Fucoxanthin, a marine carotenoid widely distributed in brown algae, is known to possess various health promoting activities including anti-obesity effects in humans, but little is known about its kinetics partly because...
Estimation of Nutrient Intake in Thailand: Influence of Differences in Food Composition Tables on Estimated Intake
Objectives Food composition tables are essential to estimating nutrient intake. For the Thai diet, food composition tables have been established in Thailand and also by the Food and Agriculture Organization of the United...
The Assessment, Knowledge and Perceived Quality of Nutrition Care amongst Nurses
Purpose: This study examined ward nurses' knowledge regarding the importance of nutritional assessment, their knowledge and perceived quality of nutrition care provided in their wards. Design: The study was a quantitati...
Biogenic Amine Formation in Reformulated Cooked Sausage Without Added Nitrite
The purpose of this study was to assess the formation of biogenic amines (BA) during chilled storage (60 days) in cooked sausages made without added nitrite. Various combinations of ingredients celery, sodium lactate, ca...