A RAPID ASSAY METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF EPLERENONE IN TABLETS BY UV SPECTROPHOTOMETRY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: To develop a rapid, simple and a cheap UV spectrophotometric assay method for the estimation of Eplerenone in tablets and validate as per ICH guidelines.Methods: The optimized method uses 0.05N HCl as a solvent for the estimation of assay of Eplerenone in tablets at a detection wavelength of 245 nm.Results: System precision and intra-day precision are exemplified by relative standard deviation of 1.5% and 1.3% respectively. Method was found to be rugged as precision was found to be 1.2%. The developed method resulted in Eplerenone exhibiting linearity in the range 5-15μg/ml. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. Conclusion: A rapid, simple and a cheap UV spectrophotometric assay method was developed and validated for the estimation of Eplerenone in tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
B. Shailaja, K. Swarna, M. Afreen, A. Ashok Kumar
Keywords
Related Articles
- EP ID EP578454
- DOI -
- Views 74
- Downloads 0
TOLERANCE AGAINST HEAVY METAL TOXICITY IN CYANOBACTERIA: ROLE OF ANTIOXIDANT DEFENSE SYSTEM
The toxicity of heavy metals is reviewed with reference to Cyanobacteria. Cyanobacteria also known as blue green algae are primitive photosynthetic prokaryotic microorganism which has high economic importance due to vari...
QUANTITATIVE ANALYSIS OF Α-MANGOSTIN IN MANGOSTEEN (GARCINIA MANGOSTANA L.) PERICARP EXTRACT FROM FOUR DISTRICT OF WEST JAVA BY HPLC METHOD
Objective: The objective of this study was to determine the validity of analytical methods of α-mangostin using HPLC with UV detector and acidic methanol as mobile phase. Then, compare the level of α-mangostin and qual...
GCMS/MS ANALYSIS AND CARDIOPROTECTIVE POTENTIAL OF CUCUMIS CALLOSUS ON DOXORUBICIN INDUCED CARDIOTOXICITY IN RATS
Objective: The present investigations were undertaken to identify phytochemicals by GC MS/MS, to evaluate the cardioprotective and antioxidant activity of the ethanolic extract of leaves of Cucumis Callosus against DOX-i...
LIPID LOWERING ACTIVITY OF FERONIA LIMONIA LEAF IN TRITON WR-1339 (TYLOXAPOL) INDUCED HYPERLIPIDEMIC RATS
Objectives: The present study was undertaken to assess the lipid lowering activity of methanolic extract from Feronia limonia leaf in Triton WR-1339 induced hyperlipidemic rats. Methods: Hyperlipidemia was induced in rat...
FORMULATION AND EVALUATION OF SUSTAINED RELEASE BILAYER TABLETS OF PROPRANOLOL HYDROCHLORIDE
Objective: The present study was to formulate and evaluate of sustained release bilayer tablets of anti-hypertensive drugs propranolol hydrochloride.Methods: The tablets were prepared by direct compression method by usin...