A RAPID METHOD FOR RICE GENOMIC DNA EXTRACTION WITHOUT LIQUID NITROGEN AND PHENOL
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 52
Abstract
A rapid, safe and inexpensive method for good quality DNA extraction from rice leaves is the basic requirement for molecular breeding in rice. Here we described a faster, safe and inexpensive method for genomic DNA extraction from rice leaves after modification of CTAB method. In the described method liquid nitrogen and Phenol is fully avoided for DNA extraction. This method yields approximately 110 ng/µl-420 ng/µl of DNA from 300 mg of fresh leaves tissue, depending on rice varieties and grinding of leaf samples. It can be completed within 4-5 hrs. The extracted DNA is stable and it can be used for DNA fingerprinting, quantitative traits loci analysis, marker-assisted selection, and genetic diversity analysis.
Authors and Affiliations
M. Z. SHAMIM
Keywords
Related Articles
- EP ID EP171059
- DOI -
- Views 127
- Downloads 0
CORRELATION AND PATH ANALYSES FOR FRUIT YIELD AND ITS COMPONENT TRAITS IN OKRA [Abelmoschus esculentus L. Moench] GENOTYPES
In the present study twenty diverse okra genotypes were used to estimate the correlation and path analysis for fruit yield and its contributing characters. The genotypes were evaluated for the following fifteen character...
EFFECT OF FOLIAR APPLICATION OF PLANT GROWTH RETARDANTS ON GROWTH, YIELD AND YIELD ATTRIBUTING PARAMETERS OF SOYBEAN (Glycine max L.) MERRILL
Plant growth regulators (PGRS) are known to improve physiological efficiency including photosynthetic ability of plants and offer significant role in realizing higher crop yields. In present study, field experiments were...
GENETIC VARIABILITY STUDIES AND MULTIVARIATE ANALYSIS IN F2 SEGREGATING POPULATIONS INVOLVING MEDICINAL RICE (Oryza sativa L.) CULTIVAR KAVUNI
In order to evaluate the relationship between morphological characters in rice cultivars, two populations at F2 generation were examined. Statistical analysis on fifteen biometrical or quantitative characters were studie...
CANAL BASED IRRIGATION SCHEDULING AND CONJUNCTIVE WATER USE PLANNING FOR OPTIMAL CROPPING PATTERN-A REVIEW
The success of water delivery in canal irrigation projects is measured based on whether water is delivered according to the required water delivery targets in an adequate, dependable, efficient and equitable fashion. One...
INFLUENCE OF PHOSPHAMIDON ON MICROBIAL BIOMASS CARBON, FDA AND SOIL RESPIRATION IN BLACK SOIL (ENTIC CHROMUSTERTS)
Phosphamidon [2-chloro-2-diethylcarbamoyl-1-methylvinyldimethyl phosphate], is a broad spectrum, non-cumulative systemic organophosphorus pesticide widely used in agriculture. The potential harmful effect on soil microbi...